33results about How to "Sensitive and accurate test results" patented technology

The invention discloses a method for quantitatively analyzing nicotine-induced cell DNA damage based on the high content technology. The method includes the steps of firstly, culturing cells; infecting the cells; thirdly, performing immunofluorescent labeling of gamma H2AX; fourthly, performing high-content-technology quantitative analysis. The method has the advantages that a high-content imaging system is used to perform automatic imaging, DNA double-strand break marker gamma H2AX protein generated by nicotine induction is quantitatively analyzed, direct and fast cell detection is achieved, and sample processing convenience is achieved; the high-resolution imaging allows the distribution of the gamma H2AX in each cell nucleus to be able to be observed directly and image data to be convenient to store and reanalyze, the nicotine-induced gamma H2AX in each cell nucleus can be quantitatively analyzed, and the detection result is sensitive and accurate.

The invention discloses an electrochemistry ampere current detection method for quantitively detecting a polymerase chain reaction (PCR) amplification product based on a methylene blue deoxyribonucleic acid (DNA) indicator. The method is applied to the PCR product quantitive detection of a mitochondria actual sample of a normal person. The method includes that the design and synthesis of primers required by the PCR amplification are performed according to amplified fragments of genes to be detected; a mitochondria gene sequence of the normal person is used as an example; a preprocessed glassy carbon electrode is placed in a PCR system containing amplification specimens, and a certain quantity of DNA hybridization indicator methylene blue is added in the system; and corresponding PCR reaction conditions are set according to target segments, the amplification is performed, and simultaneously current signal changes of the methylene blue are collected according to an electrochemistry current-time curve method so that the electrochemistry detection of the PCR amplification product is achieved. The method is high in selectivity and sensitivity, the quantitive detection of the PCR product is achieved, and compared with traditional methods, and the method is fast, simple, convenient and economical.

The invention discloses a two-step method for preparing nano-porous gold and application of nano-porous gold in detection of non-small cell lung cancer drug resistance related micro RNA (let-7a). Themethod is characterized in that the surface of a gold electrode is etched by adopting a square wave voltammetry from low frequency to high frequency to prepare a nano-porous gold (NPG) membrane modified gold electrode, thionine (Th) is used as an electrochemical hybridization indicator, and whether let-7a is contained in a to-be-detected liquid or not is judged according to the difference of electric signals, so that quantitative analysis of let-7a is realized. The two-step square wave voltammetry adopted by the method has the advantages of simple, rapid and green preparation process and the like; the prepared nano-porous gold interface has good electrical conductivity, electro-catalytic ability and biocompatibility; the large surface area can increase the fixing amount of a capture probe;an electrochemical biosensor constructed based on the electrode has the characteristics of good selectivity and high sensitivity; and compared with a traditional method, the method is faster, simpler, more convenient and more economical, and the electrode loss is small.

The invention discloses carbon dots for detecting progesterone and a progesterone detection method. The carbon dots are prepared through the following steps of: (1) putting curcumin into a container, and heating to obtain an intermediate product; (2) cooling the intermediate product to room temperature, dissolving the intermediate product in ethanol, and centrifuging; and (3) dialyzing the obtained centrifugate by using a dialysis bag, and freeze-drying the dialyzed product to obtain the carbon dots. The prepared carbon dots are good in stability, high in luminous intensity, simple to prepare and low in cost, and progesterone can specifically enhance fluorescence of the carbon dots. The progesterone detection method established by the carbon dots has the characteristics of low detection limit, accurate and reliable detection and high detection speed, can provide sensitive and accurate detection results, and has a wide application prospect in the field of progesterone specific detection.

The invention discloses a quantitative detection method for specifically detecting the mature microRNA expression level and a kit and belongs to the technical field of molecular biology. The quantitative detection method include the following steps that 1, the microRNA of a sample is extracted, and cDNA is obtained under the effects of reverse transcriptase, Poly A polymerase or Poly U polymerase through reverse transcription; 2, an upstream primer only for a mature microRNA sequence is designed according to the mature microRNA sequence and a pre-microRNA sequence, and then real-time quantitative PCR is performed to specifically detect the mature microRNA expression level. The detection process of the quantitative detection method is simple and convenient, a detection result is sensitive and accurate, the quantitative detection method can be effectively used for detecting the expression level of the mature microRNA (mature microRNA, the length is equal to 20-23 basic groups) within a cell or tissue, and the specificity for mature microRNA detection is higher than those of currently and widely used other methods based on the real-time quantitative PCR.

The invention discloses a detecting method for the authenticity of food and agricultural products based on protein quantitative detection. The detecting method comprises the following steps: firstly,detecting total protein content P of a to-be-detected product; secondly, detecting the content Pi of one or more of endogenous proteins in the to-be-detected product; thirdly, calculating the proportion Eta of total content of the detected endogenous proteins in the to-be-detected product accounting for the total protein content according to the detection results in the step one and the step two;fourthly, detecting a standard product by using the same method as step one to three to obtain total protein content P' of the standard product and the proportion Eta' of total content of the detectedendogenous proteins accounting for the total protein content; fifthly, respectively comparing P with P', and Eta with Eta' and judging the authenticity of the to-be-detected product according to comparison results. The detecting method disclosed by the invention discards the inherent lagged 'adulteration detection' idea and starts from the angle of 'real detection'; the detection idea is forward-looking, and the denounce of lagging detection is avoided.

The invention relates to a method for detecting the activity of benzyl alcohol acetyltransferase. According to the method provided by the invention, a reaction mechanism that DTNB (5,5'-dithiobis-(2-nitrobenzoic acid) can have a mutual effect with sulfydryl to form colored thiophenol anions; the activity of the benzyl alcohol acetyltransferase in a protein sample is determined through detecting the generation amount of the sulfydryl in a reaction product. The method provided by the invention has the advantages that experiment operation steps are simple, the dosage of the sample is relatively less and a detection result is sensitive and accurate. When the method provided by the invention is applied, the efficient benzyl alcohol acetyltransferase can be relatively easily screened from different protein samples.

The invention discloses a method for quantitatively detecting 1,3-butadiene-induced cell DNA damage in combination with a gas-liquid interface exposure system and a high-content technology. The method comprises the following steps: 1) cell culture; 2) 1,3-butadiene exposure through a gas-liquid interface exposure mode; 3) immunofluorescent labeling of gamma H2AX; 4) high-content detection. The method disclosed by the invention has the advantages that the efficient exposure of gaseous 1,3-butadiene to wall-attached cells in vivo is realized by adopting the in-vitro gas-liquid interface exposure mode, so that the exposure efficiency of the 1,3-butadiene is improved; a high-content imaging system is utilized for automatic imaging, 1,3-butadiene-induced DNA double-chain fracture maker gamma H2AX proteins are quantitatively analyzed, and the direct and quick detection of the cells is realized, so that the sample treatment is more convenient; through high-resolution imaging, not only can the distribution of the gamma H2AX in cell nucleuses be directly observed, but also image data is convenient to store and re-analyze, and the quantitative analysis on the 1,3-butadiene-induced gamma H2AX in each of the cell nucleuses can be realized, so that detection results are more sensitive and accurate.

The invention provides a nanoparticle solution. The nanoparticle solution comprises polymer micelle nanoparticles with blood stability, in particular nanoparticles modified with functional molecules such as nucleotide sequences. The invention also provides a method of preparing the nanoparticle solution, and a method of detecting a micro RNA (miRNA) marker using the nanoparticle solution.

The invention discloses a carbon point for detecting progesterone and a progesterone detection method. The carbon point is prepared through the following steps: 1) putting curcumin in a container and heating to obtain an intermediate product; 2) cooling the intermediate product After reaching room temperature, dissolve in ethanol and centrifuge; 3) The obtained centrifugate is dialyzed with a dialysis bag, and the dialyzed product is then freeze-dried to obtain carbon dots. The carbon dots prepared by the invention have good stability, high luminous intensity, simple preparation and low cost, and progesterone can specifically enhance the fluorescence of the carbon dots; the progesterone detection method established by the carbon dots in the present invention has low detection limit, high Accurate and reliable, fast detection speed, can provide sensitive and accurate detection results, and has broad application prospects in the field of progesterone specific detection.

The invention provides a nanoparticle solution which contains polymer micellar nanoparticles having blood stability, especially nanoparticles modified with functional molecules such as nucleotide sequences. The invention also provides a method for preparing the nanoparticle solution and a method for detecting microRNA (miRNA) markers using the nanoparticle solution.

The invention discloses a silicon quantum dot for detecting chlorogenic acid and a method for detecting chlorogenic acid. The silicon quantum dot is prepared through the following steps: 1) APTES is added to ultrapure water to obtain A solution; 2) Dissolve o-phenylenediamine in ethanol to obtain B solution; 3) Mix A solution, B solution and PEI into the reaction vessel and heat for reaction; 4) After the reaction is completed, cool to room temperature and obtain the product by dialysis The bags were dialyzed and then freeze-dried to yield silicon quantum dots. The silicon quantum dots prepared by the invention have the characteristics of fluorescence quenching for chlorogenic acid, and also have the advantages of good stability and high luminous intensity; the detection method of chlorogenic acid established by the silicon quantum dots of the invention has high efficiency, sensitivity and specificity It has strong characteristics, can provide sensitive and accurate detection results, and has broad application prospects in the field of specific detection of chlorogenic acid.

The invention discloses an electrochemistry ampere current detection method for quantitively detecting a polymerase chain reaction (PCR) amplification product based on a methylene blue deoxyribonucleic acid (DNA) indicator. The method is applied to the PCR product quantitive detection of a mitochondria actual sample of a normal person. The method includes that the design and synthesis of primers required by the PCR amplification are performed according to amplified fragments of genes to be detected; a mitochondria gene sequence of the normal person is used as an example; a preprocessed glassy carbon electrode is placed in a PCR system containing amplification specimens, and a certain quantity of DNA hybridization indicator methylene blue is added in the system; and corresponding PCR reaction conditions are set according to target segments, the amplification is performed, and simultaneously current signal changes of the methylene blue are collected according to an electrochemistry current-time curve method so that the electrochemistry detection of the PCR amplification product is achieved. The method is high in selectivity and sensitivity, the quantitive detection of the PCR product is achieved, and compared with traditional methods, and the method is fast, simple, convenient and economical.

The invention discloses a method for quantitative detection of carbon monoxide induced cell DNA injury by adopting a gas-liquid interface exposure system combined with high content technique, which comprises the following steps: (1) cell culture; (2) carbon monoxide poisoning by gas-liquid interface exposure; (3) gamma-H2AX immunofluorescence labeling; and (4) high content detection. The method disclosed by the invention has the following advantages: high efficiency poisoning of gaseous carbon monoxide to in vitro attached cells is realized by in vitro gas-liquid interface exposure, so as to improve carbon monoxide poisoning efficiency; DNA double strand break biomarker gamma-H2AX protein produced under carbon monoxide induction is quantitatively analyzed by using automatic imaging of a high content imaging system, so that direct and rapid detection of cells is realized, and sample treatment is more convenient; and high resolution imaging not only realizes visual observation of the distribution of gamma-H2AX in nuclei, but also enables image data to be convenient for storage and re-analysis, and also can realize quantitative analysis of the gamma-H2AX induced by carbon monoxide in each cell nucleus, and the detection result is more sensitive and accurate.

The invention discloses a detection analysis method of benzophenone substances in environmental water. Through the method, the benzophenone substances in the water can be simultaneously detected through the pretreatment method of solid phase extraction in combination with a high-performance liquid chromatography-mass spectrometer technology. The method comprises the steps that first, after a watersample is filtered, HCl is used to regulate the PH of the water sample, and an internal standard substance is added; second, a solid phase extraction column is used to extract, enrich and purify thebenzophenone substances in the sample; and third, the content of a target substance in the water is detected through a high-performance liquid chromatography-mass spectrometer. According to the method, the treatment method of the water sample is low in cost, the steps are simple and easy to implement, operation is easy and convenient, the detection result is accurate and has high stability, and the sample suitable for high-performance liquid chromatography-mass spectrometer detection can be quickly obtained. The method has the advantages that detection speed is high, the automatic degree is high, response is sensitive, the recovery rate can reach 71.0-120.5%, large-scale industrial application is facilitated, and the method is a rapid, efficient and accurate qualitative and quantitative detection method.

The invention discloses a photoelectric detection device and method for identifying scotch tape on banknotes based on laser reflection. Drive circuit, several plane mirrors, laser sensor array, signal processing circuit; the laser emitted by the laser tube array is irradiated on the banknote to be detected through the opening, and the reflected light of the banknote to be detected is irradiated on the laser sensor array after being reflected by several plane mirrors; The signal processing circuit is respectively connected with the laser tube drive circuit and the laser sensor array, and is used to send a control signal to the laser tube drive circuit, convert the laser sensor array signal into a corresponding logic signal, and judge the banknote currently to be detected according to the logic signal. Whether transparent tape is pasted. The invention can detect banknotes stuck with scotch tape, and has the advantages of small size, high efficiency, fast speed and strong resolution ability.