Two-step method for preparing nano-porous gold and application of nano-porous gold in detection of non-small cell lung cancer drug resistance related micro RNA (let-7a)

Abstract

The invention discloses a two-step method for preparing nano-porous gold and application of nano-porous gold in detection of non-small cell lung cancer drug resistance related micro RNA (let-7a). Themethod is characterized in that the surface of a gold electrode is etched by adopting a square wave voltammetry from low frequency to high frequency to prepare a nano-porous gold (NPG) membrane modified gold electrode, thionine (Th) is used as an electrochemical hybridization indicator, and whether let-7a is contained in a to-be-detected liquid or not is judged according to the difference of electric signals, so that quantitative analysis of let-7a is realized. The two-step square wave voltammetry adopted by the method has the advantages of simple, rapid and green preparation process and the like; the prepared nano-porous gold interface has good electrical conductivity, electro-catalytic ability and biocompatibility; the large surface area can increase the fixing amount of a capture probe;an electrochemical biosensor constructed based on the electrode has the characteristics of good selectivity and high sensitivity; and compared with a traditional method, the method is faster, simpler, more convenient and more economical, and the electrode loss is small.

Description

technical field [0001] The invention relates to a method for preparing a nanoporous gold film-modified gold electrode based on a two-step square wave voltammetry method and used for detecting non-small cell lung cancer drug resistance-related microRNA (let-7a). Background technique [0002] In terms of clinical gene diagnosis and monitoring, electrochemical DNA biosensors have the advantages of simple operation, fast detection speed, high sensitivity, good specificity, and are expected to perform high-throughput biological analysis, and are widely used in specific DNA sequences or disease-related studies. Detection of mutant genes. Compared with traditional biosensing labeling techniques, label-free detection does not require chemical modification of the sample to be tested (such as DNA or protein molecules) or pre-treatment of marker insertion that may affect the sample environment, which can make up for the difficulty of labeling, cumbersome operations and Expensive and s...

AI Technical Summary

Problems solved by technology

[0007] At present, clinical genetic detection methods mainly include flow cytometry, chromosome analysis, FISH, RT-PCR, etc., but these methods have limitations such as time-consuming, low accuracy, and high price.

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