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Kit for measuring monoamine oxidase and preparation method thereof

A technology of monoamine oxidase and kit, which is applied in the fields of medicine and biochemistry, can solve the problems of complex operation and low measurement accuracy, and achieve the effect of simple operation, convenient operation and high detection accuracy

Inactive Publication Date: 2016-08-17
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a kit for measuring monoamine oxidase and its preparation method in order to overcome the defects of complex operation and low measurement accuracy in the prior art

Method used

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  • Kit for measuring monoamine oxidase and preparation method thereof
  • Kit for measuring monoamine oxidase and preparation method thereof
  • Kit for measuring monoamine oxidase and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0061] Reagent R1:

[0062] Tris buffer 100 mmol / L

[0063] Benzylamine 25 mmol / L

[0064] α-ketoglutarate 10 mmol / L

[0065] EDTA 1.0 mmol / L

[0066] Its solvent is purified water.

[0067] Reagent R2:

[0068] Tris buffer 100 mmol / L

[0069] Acetyl-CoA 1.5 mmol / L

[0070] Reduced coenzyme 1.0 mmol / L

[0071] Glutamate dehydrogenase 2.0 KU / L

[0072] Its solvent is purified water.

Embodiment 2

[0074] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0075] Reagent R1:

[0076] Tris buffer 150 mmol / L

[0077] Benzylamine 38 mmol / L

[0078] α-ketoglutarate 2mmol / L

[0079] EDTA 1.7 mmol / L

[0080] Its solvent is purified water.

[0081] Reagent R2:

[0082]Tris buffer 50 mmol / L

[0083] Acetyl-CoA 0.5 mmol / L

[0084] Reduced coenzyme 0.2 mmol / L

[0085] Glutamate dehydrogenase 3.5 KU / L

[0086] Its solvent is purified water.

Embodiment 3

[0088] Kit preparation and method of use

[0089] 1. Prepare the reagent according to the content of the following components:

[0090] Reagent R1:

[0091] Tris buffer 100 mmol / L

[0092] Benzylamine 25 mmol / L

[0093] α-ketoglutarate 10 mmol / L

[0094] EDTA 1.0 mmol / L

[0095] Its solvent is purified water.

[0096] Reagent R2:

[0097] Tris buffer 100 mmol / L

[0098] Acetyl-CoA 1.5 mmol / L

[0099] Reduced coenzyme 1.0 mmol / L

[0100] Glutamate dehydrogenase 2.0 KU / L

[0101] Its solvent is purified water.

[0102] 2. Parameter setting of automatic biochemical analyzer

[0103] (a) Detection temperature: 37°C;

[0104] (b) Detection wavelength: main wavelength 340nm, secondary wavelength 405nm;

[0105] (c) Reaction time: 8 minutes, among them, the incubation time is 5 minutes, measure and read the absorbance A1 immediately after adding the reagent R2, read the absorbance A2 after 3 minutes, and calculate the change of absorbance ΔA = A2-A1;

[0106] (d) Reacti...

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Abstract

The invention discloses a kit for measuring monoamine oxidase and a preparation method thereof. The kit comprises double liquid components including a reagent R1 and a reagent R2 independent of each other, wherein the reagent R1 comprises 50-150 mmol / L Tris buffer solution, 12-38 mmol / L benzylamine, 2-18 mmol / L alpha-ketoglutaric acid and 0.3-1.7 mmol / L EDTA, and the solvent is purified water; and the reagent R2 comprises 50-150 mmol / L Tris buffer solution, 0.5-2.5 mmol / L acetyl coenzyme A, 0.2-1.8 mmol / L reduced coenzyme and 0.5-3.5 KU / L glutamate dehydrogenase, and the solvent is purified water. The preparation method comprises the following steps: preparing the reagents according to the component contents; mixing a to-be-measured sample with the reagent R1 and reagent R2 for sufficient reaction; measuring the absorbance difference after reaction by a full-automatic biochemical analyzer; and calculating the concentration of monoamine oxidase in the sample according to the absorbance change. The kit and preparation method thereof disclosed by the invention have the advantages of convenient operation, high accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring monoamine oxidase and a preparation method thereof. Background technique [0002] Monoamine oxidase (MAO) is an enzyme that catalyzes the oxidative deamination reaction of monoamine substances. Monoamine oxidase exists on the mitochondrial outer membrane of cells and is widely distributed in the human body, especially in liver, brain and kidney tissue cells. Because it requires flavin adenine dinucleotide as a cofactor, it belongs to the flavoprotease or flavoamine-containing oxidase. [0003] Clinically, the level of MAO activity reflects the degree of liver fibrosis and is an important indicator for diagnosing liver cirrhosis, and the increase of serum monoamine oxidase activity is parallel to the process of liver surface nodule formation, and the increase of MAO activity can also be seen in the combination of hyperthyroidism and diabetes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/32C12Q1/26
CPCC12Q1/32C12Q1/26G01N2333/90638
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD