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Carcino-embryonic antigen electrochemical sensor constructed from magnetic material and exonuclease III

A technology of exonuclease and carcinoembryonic antigen, applied in the field of analysis and detection, can solve the problems of cumbersome process, high cost, complex electrode modification, etc.

Pending Publication Date: 2020-08-07
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a carcinoembryonic antigen electrochemical sensor constructed with magnetic materials and exonuclease III and a method for detecting carcinoembryonic antigen. The electrochemical sensor of the present invention detects carcinoembryonic antigen without electrode modification. It can quickly and accurately detect carcinoembryonic antigen, has high selectivity for carcinoembryonic antigen, and solves the technical problems of complex electrode modification, cumbersome process, high cost and difficult electrode regeneration in existing electrochemical detection methods

Method used

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  • Carcino-embryonic antigen electrochemical sensor constructed from magnetic material and exonuclease III
  • Carcino-embryonic antigen electrochemical sensor constructed from magnetic material and exonuclease III
  • Carcino-embryonic antigen electrochemical sensor constructed from magnetic material and exonuclease III

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Embodiment 1

[0043] A method for detecting carcinoembryonic antigen with a carcinoembryonic antigen electrochemical sensor constructed by magnetic materials and exonuclease III, the steps are:

[0044] a) Construction of magnetic biocomposite material Fe 3 o 4 @Au NPs-S1-S2-S3: First modify S1 to Fe 3 o 4 On @Au nanoparticles, the final concentration of S1 in the reaction system is 0.1 μM, Fe 3 o 4 The final concentration of @Au nanoparticles in the reaction system is 0.2g L -1 , and then the Fe modified with S1 3 o 4 @Au nanoparticles were co-incubated with the carcinoembryonic antigen aptamer S2 and the complementary sequence S3 of the G-rich sequence at 37°C for 120 min to construct the magnetic biocomposite Fe 3 o 4 @Au NPs-S1-S2-S3; the concentration ratio of S1 to S2 and S3 is 1:1:1; the Fe 3 o 4 @Au nanoparticles are made of Fe 3 o 4 Nanoparticles with HAuCl 4 According to the mass ratio of 1:6 synthesis;

[0045] Modification of S1 to magnetic Fe as described above 3...

Embodiment 2

[0052] A method for detecting carcinoembryonic antigen with a carcinoembryonic antigen electrochemical sensor constructed by magnetic materials and exonuclease III, the steps are:

[0053] a) Construction of magnetic biocomposite material Fe 3 o 4 @Au NPs-S1-S2-S3: first modify S1 onto magnetic Fe3O4@Au nanoparticles, the final concentration of S1 in the reaction system is 1.0 μM, Fe 3 o 4 The final concentration of @Au nanoparticles in the reaction system is 0.8g L -1 , and then the Fe modified with S1 3 o 4 @Au nanoparticles were co-incubated with the carcinoembryonic antigen aptamer S2 and the complementary sequence S3 of the G-rich sequence at 37°C for 150 min to construct the magnetic biocomposite material Fe 3 o 4 @Au NPs-S1-S2-S3; the concentration ratio of S1 to S2 and S3 is 1:1:1; the Fe 3 o 4 @Au nanoparticles are made of Fe 3 o 4 Nanoparticles with HAuCl 4 According to the mass ratio of 1:3 synthesis;

[0054] Modification of S1 to magnetic Fe as describ...

Embodiment 3

[0061] A method for detecting carcinoembryonic antigen with a carcinoembryonic antigen electrochemical sensor constructed by magnetic materials and exonuclease III, the steps are:

[0062] a) Construction of magnetic biocomposite material Fe 3 o 4 @Au NPs-S1-S2-S3: first modify S1 to magnetic Fe 3 o 4 On @Au nanoparticles, the final concentration of S1 in the reaction system is 0.5 μM, Fe 3 o 4 The final concentration of @Au nanoparticles in the reaction system is 0.5g L -1 , and then co-incubated the Fe3O4@Au nanoparticles modified with S1 with the carcinoembryonic antigen aptamer S2 and the complementary sequence S3 of the G-rich sequence at 30 °C for 30 min to construct the magnetic biocomposite material Fe 3 o 4 @Au NPs-S1-S2-S3; the concentration ratio of S1 to S2 and S3 is 1:1:1; the Fe 3 o 4 @Au nanoparticles are made of Fe 3 o 4 Nanoparticles with HAuCl 4 According to the mass ratio of 1:7 synthesis;

[0063] Modification of S1 to magnetic Fe as described a...

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Abstract

The invention discloses a carcino-embryonic antigen electrochemical sensor constructed from a magnetic material and exonuclease III. The electrochemical sensor is constructed from a magnetic biological composite material Fe3O4@Au NPs-S1-S2-S3 under the assistance of the exonuclease III, wherein S1 is a nucleic acid sequence, S2 is a carcino-embryonic antigen aptamer, the nucleic acid sequence S1 comprises a G-rich sequence and a complementary pairing sequence of the carcino-embryonic antigen aptamer S2, and S3 is a complementary sequence of the G-rich sequence. The electrochemical sensor for detecting the carcino-embryonic antigen does not need electrode modification, can rapidly and accurately detect the carcino-embryonic antigen, has high selectivity on the carcino-embryonic antigen, andsolves the technical problems that an existing electrochemical detection method needs complex electrode modification, the process is tedious, the cost is high and electrode regeneration is difficult.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and more specifically relates to a carcinoembryonic antigen electrochemical sensor constructed with magnetic materials and exonuclease III and a method for detecting carcinoembryonic antigen. Background technique [0002] Carcinoembryonic antigen is an acidic glycoprotein with the characteristics of human embryonic antigen, which has been identified as a broad-spectrum tumor marker to indicate the presence and progression of various tumors, such as colorectal cancer, pancreatic cancer, gastric cancer, breast cancer Wait. The increase in CEA concentration occurs even before the appearance of clinical and radiological symptoms of the disease, therefore, the sensitive detection of CEA is of great significance for the early clinical diagnosis and prognosis evaluation of cancer. Since carcinoembryonic antigen itself does not produce a detection signal, its quantitative detection requir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6825C12Q1/44G01N27/327G01N27/30G01N27/416
CPCC12Q1/6825C12Q1/44G01N27/3278G01N27/308G01N27/416C12Q2525/205C12Q2521/319C12Q2563/137C12Q2565/607C12Q2563/155
Inventor 周学敏朱婉莹李晓芸翁晨园王晶杨威卢巧云严孝强马瑞文洪俊丽
Owner NANJING MEDICAL UNIV
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