74 results about "Carcinoembryonal antigen" patented technology

The invention provides preparation and application of an alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor, and belongs to the technical fields of nano function material, clinical analysis, a bioseneor technology and electrochemistry. The characteristics that platinum nanoparticle @meso-porous silicon @ graphene nanocomposite (PtNPs@m-Si@GS) is strong in conductivity, good in stability, large in specific surface area, good in biocompatibility, strong in catalytic activity and the like are utilized in preparation; an alpha fetoprotein second antibody (anti-alpha fetoprotein (AFP)) and a carcino-embryonic antigen secondary antibody (anti-carcino-embryonicantigen (CEA)) are marked, so as to prepare the marked second antibodies Ru-PtNPs@M-Si@GS/anti-AFP and luminol-PtNPs@M-Si@GS/anti-CEA; and the sensitivity of the sensor is obviously improved. Compared with other single-channel electrode sensors, alpha fetoprotein and carcino-embryonic antigen can be simultaneously detected on a same electrode at one time; the detection efficiency is obviously improved; and the alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor has important scientific significance and application value on clinical early diagnosis of hepatic carcinoma.

Synthetic polynucleotides encoding human carcinoembryonic antigen (CEA) are provided, the synthetic polynucleotides being codon-optimized for expression in a human cellular environment. The gene encoding CEA is commonly associated with the development of human carcinomas. The present invention provides compositions and methods to elicit or enhance immunity to the protein product expressed by the CEA tumor-associated antigen, wherein aberrant CEA expression is associated with a carcinoma or its development. This invention specifically provides adenoviral vector and plasmid constructs carrying codon-optimed human CEA and discloses their use in vaccines and pharmaceutical compositions for preventing and treating cancer.

The invention belongs to the technical field of biological detection, and particularly relates to a novel protein-DNA complex for detecting a carcino-embryonic antigen as well as a synthetic method and application thereof. The novel protein-DNA complex comprises a horseradish peroxidase-marked carcino-embryonic antigen secondary antibody, glucose oxidase and a DNA structure. The novel protein-DNAcomplex structure encapsulated with a great amount of enzyme-labeled secondary antibodies and glucose oxidase is established on the basis of a rolling circle replication technology and is used as enzyme cascade signal amplification probe. A 96-pore plate is used for fixing carcino-embryonic antigen monoclonal antibody molecules, after the to-be-detected carcino-embryonic antigen is added, a sandwiched structure is formed. The encapsulated glucose oxidase is used for catalyzing the glucose to be oxidized to generate H2O2, then the H2O2 is catalyzed by virtue of the horseradish peroxidase to oxidize ABTS, so that the high-sensitivity and high-specificity colorimetry detection of the carcino-embryonic antigen can be realized, and the novel protein-DNA complex has important significance for the early diagnosis and clinical research of cancers.

The invention relates to the technical field of electrochemical immunosensors, in particular to a construction method of a nanoprobe C60 based electrochemical immunosensor for carcino-embryonic antigens.The method includes: modifying gold nanoclusters on the surface of a glassy carbon electrode by means of cyclic voltammetry to obtain an antibody capture substrate; sequentially immobilizing first antibodies and antigens to the surface of the electrode; immobilizing AuNPs@L-cys-C60 marked second antibodies by specific binding of the antigens and the antibodies.The gold nanoclusters have excellent biocompatibility and electrical conductivity and large surface areas and are capable of promoting electron transferring between proteins and the electrode.The AuNPs@L-cys-C60 serves as an oxidation-reduction nanoprobe, the immunosensor is used for detection of the carcino-embryonic antigens on the basis of excellent specificity between the antigens and the antibodies, and detection of the carcino-embryonic antigens is realized according to different electrochemical signals of the carcino-embryonic antigens with different concentrations.

The invention relates to the technical field of electrochemical immunosense, in particular to preparation and application of a carcino-embryonic antigen electrochemical immunosensor based on an Au@Ag@Au core-shell nano-composite (Au@Ag@Au NPs) marker. Specifically, the surface of a glassy carbon electrode is modified by nitrogen-doped graphene (NG) to prepare an antibody capture substrate, Au@Ag@Au NPs tracks and marks a carcino-embryonic antigen second antibody, the second antibody marked by Au@Ag@Au NPs is captured on the surface of the sensor through sandwich immune response, and detection of a carcino-embryonic antigen is achieved by detecting electrochemical signals of hydrogen peroxide and hydroquinone in a PBS solution.

Disclosed are fragments of oncofetal antigen, otherwise known as immature laminin receptor protein that specifically stimulate one T cell subclass. The fragments may be formulated into compositions for potentiating T cell-mediated response in mammalian cancer patients. They also have therapeutic uses in vitro.

The invention provides an anti-CEA (carcinoembrynio antigen) antibody which comprises a heavy chain variable region and a light chain variable region, wherein the complementary determining region of the heavy chain variable region comprises CDR (complementary determining region)-H1 of which the amino acid sequences are shown in SEQ ID NO.1 in the description, CDR-H2 of which the amino acid sequences are shown in SEQ ID NO.2 in the description and CDR-H3 of which the amino acid sequences are shown in SEQ ID NO.3 in the description; and the complementary determining region of the light chain variable region comprises CDR-L1 of which the amino acid sequences are shown in SEQ ID NO.4 in the description, CDR-L2 of which the amino acid sequences are shown in SEQ ID NO.5 in the description and CDR-L3 of which the amino acid sequences are shown in SEQ ID NO.6 in the description. The inventor of the invention screens CEA scFv by using a phage display technology, and thus obtains a high-affinityanti-CEA single-chain antibody. In addition, an anti-CEA chimeric antigen receptor gene is introduced into T cells by using a genetic engineering method to prepare CEA CAR-T, so that CEA CAR-T cellsare capable of specifically recognizing and killing digestive tract tumor cells expressing CEA, and thus the anti-tumor effects of the CEA CAR-T cells can be achieved.

The invention discloses a nano antibody for resisting a CD16 antigen, and a bispecific antibody containing two VHH structural domains. The nano antibody of the anti-CD16 antigen has three unique complementary determining regions (CDR1, CDR2 and CDR3). The bispecific antibody is formed by fusing two heavy chains with different sequences and is used for respectively recognizing a CD16 antigen and a carcino-embryonic antigen CEA on the surface of an NK cell. The bispecific antibody can significantly enhance the activity of antibody-mediated NK cells in killing tumor target cells.

The invention relates to test paper for fast detecting carcinoma of urinary bladder. The test paper is combined test paper capable of detecting three kinds of carcinoma of urinary bladder markers, namely, nuclear matrix protein 22 (NMP22), bladder tumor antigens (BTA stat) and carcino-embryonic antigen relevant cell adhesion molecules (CEACAM1) at the same time. The test paper comprises a three-channel bottom plate, a sample adding pad, a gold standard pad, a nitrocellulose membrane and an absorbent paper layer. The three-channel bottom plate is sequentially coated with the sampling pad, the gold standard pad, the nitrocellulose membrane and the absorbent paper layer, and NMP22, BTA, CEACAM1 resisting protein monoclonal gold-labeled antibodies are contained in the gold-labeled pad. The three-channel bottom plate achieves specimen flow division through three fine tubes, and detection of the three kinds of markers is independent from one another. The test paper is fast in detecting, high in sensitivity, high in specificity, good in stability, easy and convenient to operate, visual and reliable in result judgment and easy to master. The test paper can be used for screening of high-risk groups, can also be used for follow-up visiting of carcinoma of urinary bladder postoperative patients, and is very wide in application range.

The invention belongs to the technical field of nano functional materials, immunoassay and biosensing, and provides a preparation method and application of an immunosensor based on Au-GQD@PtPd. A sandwich type electrochemical immunosensor for carcino-embryonic antigens is prepared by taking Au-GQD@PtPd as a detection antibody marker; due to excellent biocompatibility and high catalysis property of Au-GQD@PtPd, the prepared sensor has the advantages of high specificity, high sensitivity, low detection limit and the like.

The invention discloses a combined application of a cystatin SN and a carcino-embryonic antigen (CEA), and particularly discloses the application of the cystatin SN and the CEA in preparation of a marker for detection of gastric cancer or gastrointestinal stromal tumor. The invention also discloses a capture agent of the gastric cancer or gastrointestinal stromal tumor marker and a kit containing the capture agent. The prepared kit has the advantages of good specificity, high sensitivity and the like, can be used for early diagnosis of gastric cancer or gastrointestinal stromal tumor, therapeutic effect evaluation in a therapeutic process and monitoring of metastasis and recurrence after therapy, and has test results sooner than clinical symptoms.

The invention belongs to the technical field of biomedical detection, and provides a flexible label-free nano bulge metasurface structure. The flexible label-free nano bulge metasurface structure comprises a metasurface structure chip, a biological reagent and an integrated optical fiber probe; the metasurface structure chip is sequentially composited by a substrate, a chromium film and a gold film from bottom to top, wherein the substrate is covered with a periodic nanocolumn array imprinted by a nickel die; the biological reagent comprises a 11-mercaptoundecanoic acid (MUA) solution, a phosphate buffer solution (PBS), ethyl-dimethyl-amino-propyl carbodiimide (EDC), N-hydroxysuccinimide (NHS), bovine serum albumin (BSA), a carcino-embryonic antigen (CEA) and a carcino-embryonic protein antibody (Anti-CEA); and the integrated optical fiber probe is used for measuring a reflection spectrum of the metasurface structure. The invention further provides manufacturing and sensing methods ofthe flexible label-free nano bulge metasurface structure. The metasurface structure provided by the invention is low in cost, and the biomedical function of the metasurface structure is applied to high-performance biosensors.

The invention discloses a carcino-embryonic antigen electrochemical sensor constructed from a magnetic material and exonuclease III. The electrochemical sensor is constructed from a magnetic biological composite material Fe3O4@Au NPs-S1-S2-S3 under the assistance of the exonuclease III, wherein S1 is a nucleic acid sequence, S2 is a carcino-embryonic antigen aptamer, the nucleic acid sequence S1 comprises a G-rich sequence and a complementary pairing sequence of the carcino-embryonic antigen aptamer S2, and S3 is a complementary sequence of the G-rich sequence. The electrochemical sensor for detecting the carcino-embryonic antigen does not need electrode modification, can rapidly and accurately detect the carcino-embryonic antigen, has high selectivity on the carcino-embryonic antigen, andsolves the technical problems that an existing electrochemical detection method needs complex electrode modification, the process is tedious, the cost is high and electrode regeneration is difficult.

The invention relates to an immunochromatographic assay test paper strip for quantitatively detecting a carcino-embryonic antigen (CEA) and a preparation method thereof. The immunochromatographic assay test paper strip mainly comprises a sample pad, a first combining pad, a second combining pad, a cellulose nitrate membrane, a bottom plate and an absorbent paper, wherein the sample pad is treated, the first combining pad is coated with detection magnetic beads, and the second combining pad is coated with signal magnetic beads; an IgG antibody, i.e., a quality control line and a CEA coated antibody, i.e., a detection line are fixed on the cellulose nitrate membrane. The preparation method comprises the step of sequentially stacking and combining the absorbent paper, the cellulose nitrate membrane, the combining pads and the sample pad. According to the immunochromatographic assay test paper strip for quantitatively detecting the CEA and the preparation method thereof, characteristics of the magnetic beads and characteristics of an amplifying system are integrated, the aims of specific sensitivity and quantitative detection are achieved, and the cost is low; the immunochromatographic assay test paper strip simultaneously supports detection on small samples and large samples, is simple in operation and convenient and rapid in detection and is suitable for being subjected to large-scale popularized production.

The invention relates to a preparation method of a gold nanorod-coupled horse radish peroxidase and carcino-embryonic antigen-labeled antibody. A multienzyme-labeled gold nanoparticle alpha-fetoprotein immunoprobe is prepared through the electrostatic adsorption effect of a gold nanorod, a carcino-embryonic antigen-labeled antibody and horse radish peroxidase to conduct qualitative and quantitative detection on a carcino-embryonic antigen (CEA) which is a protein marker widely existing in a digestive system cancer of an endoblast source, a novel protein marker detection method is established, and sensitivity and specificity of detection are improved. The preparation method has the advantages that the whole preparation process is simple, and the method is suitable for industrialized production; the carcino-embryonic antigen can be qualitatively and quantitatively detected, and the specificity is good; the whole detection process is low in cost, easy and convenient to operate and suitable for community tumor screening of a high risk group, and a novel tumor detection method is established.

The invention discloses a kit for helping to detect a cancer antigen of sub-health people, and belongs to the technical field of biology. The kit comprises a cancer antigen calibration product, a cancer antigen quality control product, a first Tris buffer liquid, a second buffer liquid, a third Tris buffer liquid, a chemical substrate and a washing liquid, wherein the first Tris buffer liquid includes a biotin labeled cancer antigen monoclonal antibody and bovine serum albumin, the second buffer liquid includes an alkaline phosphatase labeled cancer antigen monoclonal antibody and the bovine serum albumin, and the third Tris buffer liquid includes streptavidin labeled magnetic particle and the bovine serum albumin. The kit is high in sensitivity, low in cost and rapid and reliable in reaction process and is simple to operate, no radiation pollution is generated, the sensitivity is improved, the linear range is expanded, quantitative measurement is achieved, and an appropriate theoretical judgment is provided for illness of conventional disease of the sub-health people and screening of broad-spectrum tumor.

The invention discloses a flexible electrochemical biosensor and a construction method thereof, which belong to the technical field of biochemical analysis. The method comprises the following steps: in an organic phase system, synthesizing a PEE-PPy (pentaerythritol ethoxylate-polypyrrole) membrane through electrochemical polymerization; taking the PEE-PPy membrane as a working electrode, and preparing a PPy composite film in a water phase system; adding the PPy composite film into a chloroauric acid solution for treatment, then putting the PPy composite film into a CEA (carcino-embryonic antigen) aptamer solution for treatment, and sealing to obtain the flexible electrochemical biosensor. The flexible electrochemical biosensor prepared by the invention can quantitatively detect CEA. The device has flexibility and self-supporting performance, and signals of the sensor can be kept stable under the condition of mechanical deformation.

The invention discloses a preparation method and application of a magnetic control fluorescent biosensor. The preparation method comprises the following steps of (1) preparing a magnetic control immune probe MB-Ab; (2) preparing a liposome embedded gold cluster compound Biotin-Liposome/AuNCs (gold nano-particles); (3) preparing an aptamer modified lipidosome embedded gold cluster compound, namely, a Biotin-Liposome/AuNCs-Aptamer signal probe, (4) a Biotin-Liposome/AuNCs-Aptamer signal probe being combined with a magnetic control immune probe to construct a multiple signal amplification magnetic control fluorescence biosensor for detecting the carcino-embryonic antigen (CEA); during detection, a fluorescence signal working curve of the sensor for detecting the carcino-embryonic antigens with different concentrations being drawn firstly, and then the concentration of the carcino-embryonic antigens in a clinical serum sample being detected. The method is advantaged in that the magnetic control fluorescent biosensor prepared by the preparation method disclosed by the invention is strong in anti-interference capability, has high sensitivity and high selectivity, and can be used for detecting low-abundance tumor markers in a serum sample.