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Preparation and application of alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor

A technology of alpha-fetoprotein and carcinoembryonic antigen, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials react chemically, can solve the problems of difficult early detection and early diagnosis of liver cancer, and improve sensitivity and detection efficiency effect

Inactive Publication Date: 2013-08-14
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small size of the cancer in the early stage, the liver is hidden in the deep part of the upper abdomen, and there are ribs as a barrier, it is difficult to detect early by B-ultrasound, CT scan and other means, and the liver has a strong compensatory function, and there are often no clinical symptoms in the early stage. Early diagnosis of liver cancer poses difficulties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of PtNPsM-SiGS

[0040](1) Mix 6 mL, 2.66 mg / mL graphene oxide, 0.4 g cetyltrimethylammonium bromide (CTAB), 20 mg sodium hydroxide and 42 mL ultrapure water, sonicate for 30 min; 35°C Under magnetic stirring, add tetraethyl orthosilicate (TEOS) ethanol solution drop by drop, and react for 10 h; add 80 mL of 85% hydrazine hydrate, heat at 65°C for 3 h, and centrifuge and wash with ethanol three times; dissolve the product Stir in acetone for 24 h, centrifuge three times; dissolve the product in 50 mL of ethanol, add 150 μL of 3-aminopropyltriethoxysilane (APTES), stir for 10 h, and centrifuge; dissolve the product in 42.5 mL of water, Aminated mesoporous silicon-graphene nanocomposites (M-SiGS) were obtained.

[0041] (2) Mix 3 mL M-SiGS aqueous solution and 40 mL platinum nanoparticle solution, stir for 2 h, centrifuge twice, and dry in vacuum to obtain PtNPsM-SiGS.

[0042] The TEOS ethanol solution was prepared by dissolving 350 μL TEOS in 1....

Embodiment 2

[0043] Example 2 Preparation of PtNPsM-SiGS

[0044] (1) Mix 8 mL, 2.66 mg / mL graphene oxide, 0.5 g cetyltrimethylammonium bromide (CTAB), 20 mg sodium hydroxide and 42 mL ultrapure water, sonicate for 40 min; 40°C Under magnetic stirring, add tetraethyl orthosilicate (TEOS) ethanol solution drop by drop, and react for 11 h; add 80 mL of hydrazine hydrate with a mass fraction of 85%, heat at 70°C for 3 h, and centrifuge and wash with ethanol three times; dissolve the product Stir in acetone for 24 h, centrifuge three times; dissolve the product in 50 mL of ethanol, add 200 μL of 3-aminopropyltriethoxysilane (APTES), stir for 11 h, and centrifuge; dissolve the product in 42.5 mL of water , to obtain aminated mesoporous silicon-graphene nanocomposites (M-SiGS).

[0045] (2) Mix 4 mL M-SiGS aqueous solution and 50 mL platinum nanoparticle solution, stir for 3 h, centrifuge twice, and dry in vacuum to obtain PtNPsM-SiGS.

[0046] The TEOS ethanol solution was prepared by disso...

Embodiment 3

[0047] Example 3 Preparation of PtNPsM-SiGS

[0048] (1) Mix 10 mL, 2.66 mg / mL graphene oxide, 0.6 g cetyltrimethylammonium bromide (CTAB), 20 mg sodium hydroxide and 42 mL ultrapure water, sonicate for 50 min; 45°C Stir under magnetic force, add tetraethyl orthosilicate (TEOS) ethanol solution dropwise, and react for 12 h; add 80 mL of hydrazine hydrate with a mass fraction of 85%, heat at 75°C for 3 h, and centrifuge and wash with ethanol three times; dissolve the product Stir in acetone for 24 h, centrifuge three times; dissolve the product in 50 mL of ethanol, add 250 μL of 3-aminopropyltriethoxysilane (APTES), stir for 12 h, and centrifuge; dissolve the product in 42.5 mL of water , to obtain aminated mesoporous silicon-graphene nanocomposites (M-SiGS).

[0049] (2) Mix 5 mL M-SiGS aqueous solution and 60 mL platinum nanoparticle solution, stir for 4 h, centrifuge twice, and dry in vacuum to obtain PtNPsM-SiGS.

[0050] The TEOS ethanol solution was prepared by dissol...

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Abstract

The invention provides preparation and application of an alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor, and belongs to the technical fields of nano function material, clinical analysis, a bioseneor technology and electrochemistry. The characteristics that platinum nanoparticle @meso-porous silicon @ graphene nanocomposite (PtNPs@m-Si@GS) is strong in conductivity, good in stability, large in specific surface area, good in biocompatibility, strong in catalytic activity and the like are utilized in preparation; an alpha fetoprotein second antibody (anti-alpha fetoprotein (AFP)) and a carcino-embryonic antigen secondary antibody (anti-carcino-embryonicantigen (CEA)) are marked, so as to prepare the marked second antibodies Ru-PtNPs@M-Si@GS / anti-AFP and luminol-PtNPs@M-Si@GS / anti-CEA; and the sensitivity of the sensor is obviously improved. Compared with other single-channel electrode sensors, alpha fetoprotein and carcino-embryonic antigen can be simultaneously detected on a same electrode at one time; the detection efficiency is obviously improved; and the alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor has important scientific significance and application value on clinical early diagnosis of hepatic carcinoma.

Description

technical field [0001] The invention belongs to the fields of nano functional materials, clinical analysis, biosensing technology and electrochemical technology, and provides the preparation and application of an electrochemiluminescent sensor for alpha-fetoprotein and carcinoembryonic antigen. Background technique [0002] Early diagnosis and early treatment of tumors are the key to improving the survival rate of cancer patients. The detection of tumor markers can reflect the biological characteristics of tumors, and is of great significance for guiding treatment, assisting diagnosis, and judging prognosis. A single tumor marker may not have sufficient sensitivity, accuracy, or organ specificity. Combined detection can make up for the deficiency of single index detection, and improve the sensitivity, accuracy and specificity of tumor diagnosis. [0003] Liver cancer is a life-threatening common disease. Early detection and diagnosis are the basis and premise of early trea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 李玉阳魏琴杜斌吴丹李燕李贺马洪敏张勇
Owner UNIV OF JINAN
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