Preparation method of oncofetal antigen electrochemical immunosensor based on AuNPs-PDDA-GR composite material and application thereof
A technology of immunosensor and carcinoembryonic antigen, applied in the field of electrochemical immunosensing
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Embodiment 1
[0032] (2) Take 50 μL of 1.0 μg / mL carcinoembryonic antigen antibody (anti-CEA) standard solution and apply it dropwise on the surface of the electrode, incubate in a refrigerator at 4°C for 12 hours, wash with PBS (pH7.4) buffer solution to remove physical adsorption and obtain anti -CEA / AuNPs-PDDA-GR / GCE;
[0033] (3) Take 50 μL of bovine serum albumin (BSA) solution with a mass fraction of 1% drop-coated on the surface of the electrode, incubate at 37°C for 1 h to block the non-specific binding sites, and wash with PBS (pH 7.4) buffer solution BSA / anti-CEA / AuNPs-PDDA-GR / GCE was obtained on the electrode surface;
[0034] (4) Drop 50 μL of a series of carcinoembryonic antigen standard solutions with a concentration of 0.1pg / mL~10ng / mL for specific recognition with antibodies, incubate at 37°C for 60min, and buffer with PBS (pH7.4) The electrode surface was rinsed with the solution to prepare a carcinoembryonic antigen electrochemical immunosensor (CEA / BSA / anti-CEA / AuNPs-PDD...
Embodiment 2
[0037] (2) Take 50 μL of 2.0 μg / mL anti-CEA standard solution and drop-coat it on the surface of the electrode, incubate in a refrigerator at 4°C for 12 hours, wash with PBS (pH7.4) buffer solution to remove physical adsorption to obtain anti-CEA / AuNPs-PDDA -GR / GCE;
[0038] (3) 50 μL of BSA solution with a mass fraction of 2% was drip-coated on the electrode surface, incubated at 37°C for 1 h to block non-specific binding sites, and washed the electrode surface with PBS (pH 7.4) buffer solution to obtain BSA / anti -CEA / AuNPs-PDDA-GR / GCE;
[0039] (4) Drop 50 μL of a series of carcinoembryonic antigen standard solutions with a concentration of 0.1pg / mL~10ng / mL for specific recognition with antibodies, incubate at 37°C for 60min, and buffer with PBS (pH7.4) The electrode surface was rinsed with the solution to prepare a carcinoembryonic antigen electrochemical immunosensor (CEA / BSA / anti-CEA / AuNPs-PDDA-GR / GCE) based on AuNPs-PDDA-GR composite material.
[0040] Example 3 A meth...
Embodiment 3
[0042] (2) Take 50 μL of 2.5 μg / mL anti-CEA standard solution and drop-coat it on the surface of the electrode, incubate in a refrigerator at 4°C for 12 hours, wash with PBS (pH7.4) buffer solution to remove physical adsorption to obtain anti-CEA / AuNPs-PDDA -GR / GCE;
[0043] (3) Take 50 μL of BSA solution with a mass fraction of 3% and drop-coat it on the electrode surface, incubate at 37°C for 1 h to block the non-specific binding sites, wash the electrode surface with PBS (pH 7.4) buffer solution to obtain BSA / anti -CEA / AuNPs-PDDA-GR / GCE;
[0044] (4) Drop 50 μL of a series of carcinoembryonic antigen standard solutions with a concentration of 0.1pg / mL~10ng / mL for specific recognition with antibodies, incubate at 37°C for 60min, and buffer with PBS (pH7.4) The electrode surface was rinsed with the solution to prepare a carcinoembryonic antigen electrochemical immunosensor (CEA / BSA / anti-CEA / AuNPs-PDDA-GR / GCE) based on AuNPs-PDDA-GR composite material.
[0045] Example 4 Pre...
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