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Oncofetal antigen determination kit

A carcinoembryonic antigen and kit technology, applied in the fields of medicine and biochemistry, can solve the problems of complex operation of carcinoembryonic antigen and low measurement accuracy, and achieve the effects of good detection accuracy, high detection sensitivity and convenient detection.

Inactive Publication Date: 2016-11-09
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problems of complex operation and low measurement accuracy in the detection process of carcinoembryonic antigen in the prior art, and provide a kit for measuring rheumatoid factor

Method used

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  • Oncofetal antigen determination kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0075] Reagent R1:

[0076] MOPS buffer 100 mmol / L

[0077] Sodium chloride 225 mmol / L

[0078] Polyethylene glycol 15g / L

[0079] Bovine Serum Albumin 30 g / L

[0080] Sodium azide 0.6 g / L

[0081] Its solvent is purified water.

[0082] Reagent R2:

[0083] MOPS buffer 100 mmol / L

[0084] Sodium chloride 200 mmol / L

[0085] Bovine Serum Albumin 11 g / L

[0086] Trehalose 20 g / L

[0087] Sodium azide 0.6 g / L

[0088] Latex-coated anti-human carcinoembryonic antigen monoclonal antibody 1.0 %

[0089] Its solvent is purified water.

Embodiment 2

[0091] Kit preparation and method of use

[0092] 1. Production of latex-coated anti-human carcinoembryonic antigen monoclonal antibody:

[0093] (a) Dilute latex particles with a particle size of 80nm to 3 g / L with 50 mmol / L MES buffer, add 1.0 mg of EDAC to each mL of solution, react at room temperature for 2 hours, and centrifuge at 15000 rpm Centrifuge at high speed for 30 minutes, remove the supernatant, suspend the pellet in 50 mmol / L MES buffer, and disperse by ultrasonic;

[0094] (b) Centrifuge the product of step (a) at 20,000 rpm for 30 minutes in a centrifuge, discard the supernatant, and suspend the precipitate in 50 mmol / L MES buffer, so that the final concentration of latex particles is 6.0 g / L, Ultrasonic dispersion, while stirring, add an equal volume of MES diluent containing 8 mg / mL anti-human carcinoembryonic antigen antibody, mix and stir, react at room temperature for 2 hours, and the final concentration of latex particles is 3.0 g / L;

[0095] (c) Centr...

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Abstract

The invention relates to the technical fields of medical science and biochemistry, and concretely relates to an oncofetal antigen determination kit. The purpose of the invention is to solve the problems of complex operation and low determination accuracy of the oncofetal antigen detection process in the prior art. A technical scheme provided by the invention to solve the problems is characterized in that the oncofetal antigen determination kit comprises a reagent R1 and a reagent R2 which are liquid components, wherein the R1 reagent comprises 15-185 mmol / L of an MOPS buffer solution, 150-400 mmol / L of sodium chloride, 5-25 g / L of polyethylene glycol, 18-42 g / L of bovine serum albumins, 0.3-0.9 g / L of sodium azide, and a solvent being purified water, and the reagent R2 comprises 50-150 mmol / L of the MOPS buffer solution, 100-300 mmol / L of sodium chloride, 4-18 g / L of the bovine serum albumins, 5-35 g / L of trehalose, 0.3-0.9 g / L of sodium azide, 0.1-2.0% of a latex coated anti-human oncofetal antigen monoclonal antibody, and the solvent being purified water. The kit has the advantages of high accuracy and convenience in operation.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring carcinoembryonic antigen. Background technique [0002] Carcinoembryonic antigen is a glycoprotein produced by colorectal cancer tissue, which can cause immune response in patients as an antigen. This kind of antigen is called carcinoembryonic antigen, which can be widely found in digestive system cancers of endoderm origin, also in the digestive tract tissues of normal embryos, and can also exist in trace amounts in normal human serum. Carcinoembryonic antigen is a broad-spectrum tumor marker, which can reflect the existence of a variety of tumors to people. It is a good tumor marker for the judgment of curative effect, disease development, monitoring and prognosis estimation of colorectal cancer, breast cancer and lung cancer thing. [0003] CEA was originally found in colon cancer and fetal intestinal tissue, hence the name carcinoembry...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/574
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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