Sensor based on hybridization chain reaction and ribozyme and carcino-embryonic antigen detection method
A hybrid chain reaction, carcinoembryonic antigen technology, applied in the field of chemical and biological sensing, can solve the problems of harsh experimental conditions, high cost, low sensitivity, etc., to improve selectivity and sensitivity, reduce detection costs, and shorten detection time. Effect
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Embodiment 1
[0055] The nucleic acid aptamer probe, the first hairpin probe, the second hairpin probe and carcinoembryonic antigen were centrifuged at 10,000rpm and 30s at 4°C before opening the cover for the first time, and then dissolved in secondary water to make the concentration 100 μM mother solution, and then diluted with Tris-HCl buffer to form a nucleic acid aptamer probe solution, a first hairpin probe solution and a second hairpin probe solution, and stored at 4°C until use; the nucleic acid aptamer probe The needle solution 5 μM, the first hairpin probe solution 5 μM and the second hairpin probe solution 5 μM were respectively heated to 90° C., reacted for 10 minutes, and cooled to room temperature for use.
[0056] Among them, in figure 2 In (a), add Tris-HCl buffer solution with a pH of 7.4 to the centrifuge tube, and react at 37°C for 6 hours; 2 HPO 4 -NaOH buffer, react at room temperature for 20 minutes.
[0057] exist figure 2 In (b), Tris-HCl buffer solution with a...
Embodiment 2
[0063] The nucleic acid aptamer probe, the first hairpin probe, the second hairpin probe and the carcinoembryonic antigen are centrifuged at 10,000 rpm and 4°C for 30 seconds before opening the cover for the first time, and then dissolved in secondary water to make 100 μM Dilute the mother solution with Tris-HCl buffer and store at 4°C until use; heat the aptamer probe solution 5 μM, the first hairpin probe solution 5 μM and the second hairpin probe solution 5 μM to 90°C respectively , reacted for 10 minutes, and cooled to room temperature for later use.
[0064] Among them, in image 3 In (a), add 50nM nucleic acid aptamer probe solution, 50nM first hairpin probe solution and 50nM second hairpin probe solution to the centrifuge tube in Tris-HCl buffer solution with pH 7.4, and react at 37°C 6 hours to obtain a mixed solution; then add 0.5 μM hemin, react at room temperature for 2 hours to obtain a reaction solution; then take out 100 μL of the reaction solution and add 6 mM ...
Embodiment 3
[0075] The nucleic acid aptamer probe, the first hairpin probe, the second hairpin probe and carcinoembryonic antigen were centrifuged at 10,000rpm and 4°C for 30s before being used for the first time, and were dissolved in secondary water to prepare 100μM The mother solution was diluted with Tris-HCl buffer solution and stored at 4°C until use; the aptamer probe solution 5 μM, the first hairpin probe solution 5 μM and the second hairpin probe solution 5 μM were heated to 90°C, React for 10 minutes, and cool to room temperature for later use.
[0076] exist Figure 5 In (a), add 50nM nucleic acid aptamer probe solution, 50nM first hairpin probe solution, 50nM second hairpin probe solution and 0.25nM immunoglobulin G (IgG) to the centrifuge tube at pH 7.4 In Tris-HCl buffer solution, react at 37°C for 6 hours to obtain a mixed solution; then add 0.5 μM hemin, react at room temperature for 2 hours to obtain a reaction solution; then take out 100 μL of the reaction solution and ...
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