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Carbon dot for detecting progesterone and progesterone detection method

A detection method and progesterone technology, applied in the field of nanomaterials, can solve the problems of long sample pretreatment time, low sensitivity, long running time, etc., and achieve the effects of fast detection speed, broad application prospects and low detection limit.

Active Publication Date: 2022-06-17
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Methods for detecting progesterone include chromatographic analysis, colorimetry, electrochemical methods, etc. However, these methods have certain disadvantages, such as long sample pretreatment time, expensive reagents, long running time, and low sensitivity

Method used

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  • Carbon dot for detecting progesterone and progesterone detection method
  • Carbon dot for detecting progesterone and progesterone detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The present embodiment provides a carbon dot for detecting progesterone, which is prepared by the following steps:

[0042] 1) 0.5g curcumin was put into a beaker, heated at 180° C. for 6 hours in a reaction kettle, and a brown intermediate product was obtained;

[0043] 2) After the intermediate product was naturally cooled to room temperature, it was dissolved in ethanol and centrifuged at 5000 rpm for 30 minutes;

[0044] 3) The obtained centrifuge is dialyzed with a dialysis bag to remove unreacted small molecules, and the dialyzed product is then freeze-dried to obtain carbon dots.

[0045] refer to figure 1 , figure 1 A is the absorption spectrum of the carbon dots prepared in Example 1, figure 1 B is the emission spectrum of carbon dots under different wavelengths of excitation light, figure 1 In B, at the arrow position, the wavelengths increase sequentially from top to bottom, that is, the wavelengths of the curve here are 360, 380, 400, 420, 440, 460, and ...

Embodiment 2

[0047] This implementation provides a method for detecting progesterone, which uses the carbon dots prepared in Example 1 for detection, and the specific detection steps include:

[0048] 1) Construct a standard curve for progesterone detection:

[0049] Mix n parts of the progesterone solution with a certain concentration gradient each with a volume of v1 and a carbon dot solution with a volume of v2 and a concentration of c, and let stand;

[0050] The obtained n parts of the mixed solution are respectively at excitation wavelength of 410nm, and the fluorescence intensity value of the mixed solution is tested;

[0051] Taking the progesterone concentration as the abscissa and the fluorescence intensity as the ordinate, the standard curve for progesterone detection was obtained by fitting;

[0052] 2) Detect the progesterone concentration in the solution to be tested:

[0053] Mix the solution to be tested with volume v1 and the carbon dot solution with volume v2 and concen...

Embodiment 3

[0067] In this example, the interference experiment of carbon dots for the detection of progesterone concentration was performed.

[0068] In this embodiment, the interfering substances used include Cu 2+ , Fe 3+ ,K + ,Na + ,Pb 2+ ,Hg 2+ , Al 3+ ,Mn 2+ ,Ca 2+ ,AA,Cys,H 2 O 2 , the concentration is 120μmol / L. The experimental method is as follows: mix the carbon dots with 120 μmol / L interfering substance solution, and at the same time mix the same concentration of carbon dots with progesterone with a concentration of 120 μmol / L as a comparison, let stand for 3 minutes; then at the excitation wavelength of 350 nm, test Fluorescence intensity values ​​of two mixed solutions; the above experiments were performed separately for all interferents. refer to figure 2 D, is the experimental result, in which P4 represents progesterone. It can be seen that only progesterone has an enhanced effect on the fluorescence of the Cdot solution, while other interfering substances hav...

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Abstract

The invention discloses a carbon point for detecting progesterone and a progesterone detection method. The carbon point is prepared through the following steps: 1) putting curcumin in a container and heating to obtain an intermediate product; 2) cooling the intermediate product After reaching room temperature, dissolve in ethanol and centrifuge; 3) The obtained centrifugate is dialyzed with a dialysis bag, and the dialyzed product is then freeze-dried to obtain carbon dots. The carbon dots prepared by the invention have good stability, high luminous intensity, simple preparation and low cost, and progesterone can specifically enhance the fluorescence of the carbon dots; the progesterone detection method established by the carbon dots in the present invention has low detection limit, high Accurate and reliable, fast detection speed, can provide sensitive and accurate detection results, and has broad application prospects in the field of progesterone specific detection.

Description

technical field [0001] The invention relates to the field of nanomaterials, in particular to a carbon dot for detecting progesterone and a method for detecting progesterone. Background technique [0002] Progesterone (P4), a steroid hormone secreted by the ovary, plays a vital role in maintaining and regulating physiological processes such as the female menstrual cycle, embryonic development, pregnancy and breast development, and is also used in human medicines and clinical treatments. is also widely used. However, misuse of progesterone may cause adverse side effects such as weakness, depression, breast discomfort, and certain potential carcinogenic effects. The sensitivity analysis of progesterone monitoring in clinical medicine, animal husbandry and environmental protection has high requirements. Therefore, the efficient and sensitive detection of progesterone is of great significance. [0003] Methods for detecting progesterone include chromatography, colorimetry, ele...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/6428
Inventor 昝明辉梅茜董文飞安帅吴再辉刘莹葛明锋李力
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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