Method for hydrolyzing adenosine through cation exchange resin catalysis-separation coupled technology

Abstract

The invention discloses a method for hydrolyzing adenosine through cation exchange resin catalysis-separation coupled technology and belongs to the technical field of biochemical engineering. According to the method for hydrolyzing the adenosine through the cation exchange resin catalysis-separation coupled technology, catalysis-absorption coupled interaction of cation exchange resin is applied tocutting off carbon-hydrogen bonds in the molecular structure of adenosine and then efficiently hydrolyzing the adenosine to obtain adenine and meanwhile purify mother liquor containing D-ribose, thereby facilitating crystallization of D-ribose. The method for hydrolyzing the adenosine through the cation exchange resin catalysis-separation coupled technology can effectively reduce the production cost of the adenine and the D-ribose and improve the production efficiency and is applicable to scale production.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and specifically relates to a method for preparing adenine and D-ribose by hydrolyzing adenine nucleoside by using cation exchange resin catalysis-separation coupling technology. Background technique [0002] Adenine, also known as 6-aminopurine, is an important pharmaceutical raw material and intermediate. It can be used to produce anti-hepatitis B drugs adefovir dipivoxil, tenofovir dipivoxil, plant cell growth hormone 6-benzylaminopurine, vitamin B4, etc. Because adenine has such a wide range of uses, the amount used in the world has been continuously expanding in recent years. Therefore, the development of a green, environmentally friendly and economical method for the industrialization of adenine has aroused the interest of chemical and biological workers. D-ribose is an important five-carbon monosaccharide. It is an important component of ribonucleic acid (RNA), the genetic ma...

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Problems solved by technology

[0005] Route 1, the preparation method disclosed in Chinese patent document CN 103923083A, uses adenosine as a substrate to obtain acetyl adenine and tetraacetyl ribose after acetylation with acetic anhydride, and acetyl adenine is further hydrolyzed to obtain adenine, and theoretically four Acetyl ribose can be deacylated to generate D-ribose. Although this process route can obtain adenine and tetraacetyl ribose with a higher yield, the process is complicated, the amount of equipment used in production is large, and the quality of D-ribose obtained is very poor, and Produces more industrial three wastes

[0006] Line 2, the preparation method disclosed in the Chinese patent document CN101125854A, uses high-temperature liquid water to hydrolyze adenosine to prepare adenine and D-ribose, but the defect of this method is that the hydrolysis of adenosine needs to be carried out at high temperature, and the generated by-product D- Ribose is easily denatured at high temperature and cannot be used, which is very uneconomical

The preparation method disclosed in the Chinese patent document CN 105802938A uses adenosine hydrolase to hydrolyze adenosine to prepare adenine and D-ribose, but this method involves the preparation process of adenosine hydrolase and the subsequent separation of D- The extraction of ribose needs to remove protein, purine and unreacted adenosine. The process is quite cumbersome, resulting in complex production routes, high cost and heavy pollution.

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