Adenosine deaminase detection kit

Abstract

The invention relates to an adenosine deaminase detection kit. A vessel is contained in the detection kit and filled with liquid for detection, the liquid for detection is prepared from 50-100 mmol / L of glycine buffer solution, 50-80 mmol / L of sodium benzoate, 12 / 18 u / L of purine nucleoside phosphorylase, 30-40 u / L of xanthine oxidase, 200-300 u / L of peroxidase, 30-100 g / L of glyceraldehyde, 0.5 g / L of sodium azide, 10-200 mmol / L of disodium hydrogen phosphate, 100-300 mmol / L of KCl, 40-100 mmol / L of mannitol, 0.1-0.3 ml / L of preservative, 100-150 mmol / L of adenosine, 2-4 mmol / L of 4-aminoantipyrine, 10-15 mmol / L of color developing agent, 20-40 mmol / L of adenine nucleoside and 3-5 g / L of bovine serum albumin. The detection kit has the advantages of being high in determination precision, high in antijamming capacity, suitable for automated rapid determination and capable of creating favorable conditions for routine development of ADA detection application in clinic.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a detection kit, in particular to an adenosine deaminase detection kit. Background technique [0002] Adenosine deaminase (ADA) is a nucleic acid metabolizing enzyme that has an important relationship with the body's cellular immune activity. Determination of ADA and its isoenzyme levels in blood and body fluids for the diagnosis, differential diagnosis, treatment and immune function research of certain diseases has been paid more and more attention in clinic. ADA activity is a sensitive indicator to reflect liver damage, and can be used as one of the routine liver function inspection items. The liver enzyme spectrum combined with ALT or GGT can more comprehensively reflect the enzyme changes of liver diseases. [0003] In the past ten years, with the in-depth research on ADA, the detection methods have also been continuously developed. Four generations have been developed so ...

AI Technical Summary

Problems solved by technology

This method is also useless due to the interference of serum ammonia and non-specific oxidation caused by excessive NADPH in the test system.

For the third-generation ADA test method, the absorbance of serum at 293nm is too high, which makes it inconvenient for clinical application

The fourth-generation ADA test This method overcomes the difficulties of previous ADA tests, however, due to the high cost of reagents, it hinders practical clinical use