8 results about "Uracil nucleoside" patented technology

The invention relates to a nucleotide PET molecular probe targeting uridine phosphorylase as well as a preparation method and application of the nucleotide PET molecular probe. The PET molecular probe comprises a compound [< 18 > F] Uridine or [< 18 > F] Tripolyridine which is marked by uracil nucleosides < 18 > F. Compared with the prior art, the PET molecular probe prepared by the invention has a positioning diagnosis effect on tumor uridine metabolism change, and simultaneously realizes tumor metabolism image typing and pathological evaluation after tumor treatment.

The present invention relates to the technical field of nucleic acid tool enzymes, and particularly to a T7 RNA polymerase mutant and its preparation and application. The T7 RNA polymerase mutant of the present invention has RNA polymerase activity based on the T7 promoter. Compared with the wild-type T7 RNA polymerase, it has the advantages of strong thermal stability, high transcriptional enzyme activity, and high transcriptional product expression rate. It can also use modified nucleotides such as (m<supgt;1< / supgt;φTP) instead of uridine triphosphate (UTP) as a substrate through in vitro co-transcription to efficiently synthesize mRNA with high expression efficiency. Compared with the existing T7 RNA polymerase, its performance is significantly improved, better meeting the requirements of current large-scale mRNA production, and providing an effective candidate enzyme tool for the research and application of RNA. Moreover, the method for preserving the T7 RNA polymerase mutant provided by the present invention can enable the lyophilized powder of the T7 RNA polymerase mutant to retain more than 90% of its enzyme activity after being stored at 4°C for 1 year.

The present invention provides a method for simultaneously determining nine components in a safflower pharmaceutical composition, belonging to the field of drug detection technology. The method comprises preparing a reference solution I by taking uridine, guanosine, adenosine, p-hydroxybenzoic acid glycoside, 5-hydroxymethylfurfural, syringin, and p-hydroxysafflower yellow A; preparing a reference solution II by taking p-hydroxycinnamic acid and kaempferol 3-O-rutinoside; preparing a test solution by taking the safflower pharmaceutical composition; and performing high-performance liquid chromatography on the reference solution I, the reference solution II, and the test solution, respectively. The contents of the nine components in the safflower pharmaceutical composition are then calculated using an external standard method. The present invention utilizes a method for determining the contents of nine chemical components in a safflower pharmaceutical composition by high-performance liquid chromatography at one time, thereby achieving control of multiple index components in the safflower pharmaceutical composition.

The invention discloses a method for preparing high-purity houridine (formula 1). According to the method, a formula 44 is used as a raw material, and high-purity pseudouridine (formula 1) is prepared through condensation, reduction, cyclic etherification and deprotection in sequence. The method has the excellent effects of high yield, high purity and the like. ,

The invention relates to the technical field of nucleic acid tool enzymes, in particular to a T7RNA polymerase mutant as well as preparation and application thereof. The T7 RNA polymerase mutant disclosed by the invention has RNA polymerase activity based on a T7 promoter, and has the advantages of strong thermal stability, high transcriptase activity, high transcription product expression rate and the like compared with wild type T7 RNA polymerase; in addition, modified nucleotide such as (m1phi TP) can be used for replacing uridine triphosphate (UTP) to serve as a substrate to efficiently synthesize mRNA with high expression efficiency through in-vitro co-transcription, compared with existing T7RNA polymerase, the performance is obviously improved, the requirement for large-scale production of mRNA at present is better met, and an effective candidate enzyme tool is provided for research and application of RNA. Moreover, according to the preservation method of the T7RNA polymerase mutant, provided by the invention, after the T7RNA polymerase mutant freeze-dried powder is preserved for 1 year under the condition of 4 DEG C, the enzyme activity residue is higher than 90%.

The present application provides a method for one-pot biosynthesis of arabinonucleoside, and a composition. The method for one-pot biosynthesis of arabinonucleoside includes: mixing a substrate, uracil nucleoside phosphorylase or pyrimidine nucleoside phosphorylase, and purine nucleoside phosphorylase together, then performing biosynthesis, and directly obtaining arabinonucleoside through preparation by means of a one-pot method. The uracil nucleoside phosphorylase includes UP shown in SEQ ID NO: 1; the pyrimidine nucleoside phosphorylase includes PyNP shown in SEQ ID NO: 2; the purine nucleoside phosphorylase includes PNP shown in SEQ ID NO: 3; the substrate includes 1-beta-D-Arabinofuranosyluracil and a substrate base.

The present application provides a method for enzymatic synthesis of a nucleoside bearing a protecting group, and a composition. The method includes: using pyrimidine nucleoside phosphorylase or uracil nucleoside phosphorylase and purine nucleoside phosphorylase to catalyze a substrate to synthesize a nucleoside bearing a protecting group, where the substrate includes a substrate nucleoside, a substrate base and a substrate phosphate, and the substrate base bears a protecting group; the pyrimidine nucleoside phosphorylase includes a protein that is of more than 80% identity to a protein PyNP shown in SEQ ID NO: 1; the uracil nucleoside phosphorylase includes a protein that is of more than 80% identity to a protein UP shown in SEQ ID NO: 2; and the purine nucleoside phosphorylase includes a protein that is of more than 80% identity to a protein PNP shown in SEQ ID NO: 3, 7 or 8. The present application can solve the problem that there is no biosynthesis method to produce a nucleoside bearing a protecting group in the prior art, and thus is suitable for the field of the enzymatic synthesis.

The invention discloses a method for preparing high-purity houridine (formula 1) by adopting tert-butyl diphenyl silyl protection. According to the method, a formula 42 is used as a raw material, and the high-purity pseudouridine (formula 1) is prepared through hydroxyl protection, condensation, reduction, cyclic etherification and deprotection in sequence. The method has the advantages of high yield, high purity and the like.