5 results about "Uracil nucleoside" patented technology

The invention relates to a nucleotide PET molecular probe targeting uridine phosphorylase as well as a preparation method and application of the nucleotide PET molecular probe. The PET molecular probe comprises a compound [< 18 > F] Uridine or [< 18 > F] Tripolyridine which is marked by uracil nucleosides < 18 > F. Compared with the prior art, the PET molecular probe prepared by the invention has a positioning diagnosis effect on tumor uridine metabolism change, and simultaneously realizes tumor metabolism image typing and pathological evaluation after tumor treatment.

The invention discloses a method for preparing high-purity houridine (formula 1). According to the method, a formula 44 is used as a raw material, and high-purity pseudouridine (formula 1) is prepared through condensation, reduction, cyclic etherification and deprotection in sequence. The method has the excellent effects of high yield, high purity and the like. ,

The present application provides a method for one-pot biosynthesis of arabinonucleoside, and a composition. The method for one-pot biosynthesis of arabinonucleoside includes: mixing a substrate, uracil nucleoside phosphorylase or pyrimidine nucleoside phosphorylase, and purine nucleoside phosphorylase together, then performing biosynthesis, and directly obtaining arabinonucleoside through preparation by means of a one-pot method. The uracil nucleoside phosphorylase includes UP shown in SEQ ID NO: 1; the pyrimidine nucleoside phosphorylase includes PyNP shown in SEQ ID NO: 2; the purine nucleoside phosphorylase includes PNP shown in SEQ ID NO: 3; the substrate includes 1-beta-D-Arabinofuranosyluracil and a substrate base.

The present application provides a method for enzymatic synthesis of a nucleoside bearing a protecting group, and a composition. The method includes: using pyrimidine nucleoside phosphorylase or uracil nucleoside phosphorylase and purine nucleoside phosphorylase to catalyze a substrate to synthesize a nucleoside bearing a protecting group, where the substrate includes a substrate nucleoside, a substrate base and a substrate phosphate, and the substrate base bears a protecting group; the pyrimidine nucleoside phosphorylase includes a protein that is of more than 80% identity to a protein PyNP shown in SEQ ID NO: 1; the uracil nucleoside phosphorylase includes a protein that is of more than 80% identity to a protein UP shown in SEQ ID NO: 2; and the purine nucleoside phosphorylase includes a protein that is of more than 80% identity to a protein PNP shown in SEQ ID NO: 3, 7 or 8. The present application can solve the problem that there is no biosynthesis method to produce a nucleoside bearing a protecting group in the prior art, and thus is suitable for the field of the enzymatic synthesis.

The invention discloses a method for preparing high-purity houridine (formula 1) by adopting tert-butyl diphenyl silyl protection. According to the method, a formula 42 is used as a raw material, and the high-purity pseudouridine (formula 1) is prepared through hydroxyl protection, condensation, reduction, cyclic etherification and deprotection in sequence. The method has the advantages of high yield, high purity and the like.