The invention discloses antimicrobial disinfecting hand cream and a preparation process thereof, belonging to the technical field of daily cosmetics. Components in the hand cream comprise, by weight percentage, 0.1% to 10% of the extractive of root of large-flowered skullcap, 0.1% to 10% of the extractive of root of fructus forsythiae, 0.1% to 10% of the extractive of root of tarragon, 0.1% to 10% of the extractive of coptis, 10% to 20% of Vaseline, 5% to 15% of octadecanol, 5% to 10% of polyethylene glycol-400 (PEG-400), 5% to 10% of cetanol, 5% to 10% of glycerol monostearate, 10% to 25% of glycerol, 5% to 10% of liquid paraffin, 0.1% to 0.2% of sodium sorbate and 30% to 50% of purified water. The preparation process is as follows: the extractive of root of large-flowered skullcap, the extractive of root of fructus forsythiae, the extractive of root of tarragon, the extractive of coptis and polyethylene glycol-400 (PEG-400) are fully mixed and merged together with the rest components, and homogeneous emulsification and stirring are carried out so as to obtain the antimicrobial disinfecting hand cream. The antimicrobial disinfecting hand cream has the beneficial effects of protecting hands and killing bacteria and thoroughly blocks the propagation of bacteria.