80 results about "Monkey Pox" patented technology

The invention belongs to the technical field of biological pharmacy, and particularly relates to a monoclonal antibody combined with monkey pox virus B6R protein and application of the monoclonal antibody. The antibody is any one or a combination of more of B6R-B5, B6R-F6 and B6R-F11, each of the B6R-B5, the B6R-F6 and the B6R-F11 comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region has three heavy chain CDRs, and the light chain variable region has three light chain CDRs. The invention provides three groups of monoclonal antibodies with excellent affinity with the monkey pox virus B6R and disclosed heavy chain and light chain variable region sequences, provides technical support for preparing a detection and diagnosis reagent of the monkey pox virus, and is expected to be developed into a special product for screening and diagnosing the monkey pox virus. High application values are realized on prevention, control and treatment of the monkey pox virus.

The present invention discloses an integrated biosensor platform based on microneedles and hydrogels and its application in detecting monkeypox virus, wherein the platform comprises a three-electrode microneedle, a hydrogel, a burning needle and a micro-electrochemical workstation. The microneedle electrode of the working electrode is combined with a nanoenzyme and an A29 protein antibody, and the monkeypox virus in the sample is extracted by the specific binding of the antibody with the monkeypox virus; the burning needle is used to connect the three-electrode microneedle and the micro-electrochemical workstation and transmit electrochemical signals. After the three-electrode microneedle is inserted into the hydrogel, the nanoenzyme catalyzes the TMB contained in the hydrogel and generates a blue oxidation product, thereby generating an electrochemical signal, which is collected by the micro-electrochemical workstation through the burning needle, and the electrochemical signal is collected through Bluetooth and a hydrogel colorimetric image is obtained, thereby realizing the detection of monkeypox virus. The integrated biosensor platform of the present invention has a low detection limit and has broad application prospects in the rapid on-site detection of monkeypox virus.

The invention provides a monkey pox virus multi-antigen monovalent vaccine and application thereof. Specifically, the invention provides a monkey pox virus monovalent multi-antigen fusion protein, and the fusion protein comprises a first fusion protein element containing A35R and M1R proteins and a second fusion protein element containing A29L and B6R proteins from an N terminal to a C terminal. According to the invention, A35R, M1R, A29L and B6R are subjected to reasonable antigen combination to design a protein molecule, and the protein molecule is prepared into a monovalent mRNA vaccine. Experimental results show that the mRNA vaccine provided by the invention can ensure high-efficiency expression of target protein in vivo and can induce a high-level neutralizing antibody.

The invention discloses an anti-monkey pox virus antibody as well as a preparation method and application thereof. The invention specifically discloses eight kinds of antibodies (A35R-9E4, A35R-9F8, A35R-10C4, A35R-10D5, A35R-10E7, A35R-10H6, A35R-18E2 and A35R-18H2) which have very good affinity to monkey pox virus A35R protein, and the eight kinds of antibodies have very good neutralizing activity and a certain in-vivo protection effect.

The invention provides an ultra-sensitive and high-specificity primer pair and crRNA for instantly detecting monkey pox virus, a kit and application thereof, and belongs to the technical field of molecular biological detection. The primer pair and the crRNA disclosed by the invention comprise a front primer, a rear primer and the crRNA. According to the primer pair and the crRNA disclosed by the invention, immediate detection of the MIRRA-CRISPR-Cas13a mediated monkey pox virus can be realized, the detection sensitivity is high, the specificity is strong, and rapid, accurate and sensitive identification of the monkey pox virus can be realized. Through implementation, experiments show that the detection limit of the primer pair and the crRNA for detecting the monkey pox virus can reach 14.4 copies / mL, and the primer pair and the crRNA have important significance on accurate clinical diagnosis of the monkey pox virus and epidemiological investigation.

The invention provides an immunochromatographic test strip for quantitatively detecting monkey pox virus based on fluorescent latex microspheres, which takes europium chelate Eu (DBM) 3Phen-loaded monodisperse carboxylated polystyrene copolymer microspheres as a signal substance, and the microspheres are subjected to acid-base treatment before loading to loosen the surface structure of the microspheres so as to improve the loading capacity; the surface compound of the test strip is prepared by coupling carboxyl on the surface of the microsphere with an antibody. The invention also discloses a preparation method of the test strip. The kit is convenient for rapidly and accurately detecting the monkey pox virus, and has the characteristics of simplicity, rapidness, no toxicity and low cost.

The invention relates to the technical field of medicines, in particular to application of salinomycin in preparation of oral medicines for preventing or treating monkey pox virus (MPXV) infection. Salinomycin is a polyether antibiotic produced by actinomycetes. It is found for the first time that salinomycin can efficiently inhibit monkey pox virus infection on the cell culture level, and can significantly improve the survival rate of mice infected with monkey pox virus in small animal living bodies. More importantly, salinomycin can be administered by oral administration, has good bioavailability and safety, and provides a brand new treatment means for monkey pox virus infection. The medicinal value of salinomycin in resistance to monkey pox virus infection is reported for the first time at home and abroad, and the application prospect of salinomycin in the field of viral disease treatment is expanded.

The invention belongs to the technical field of biological detection, and particularly relates to a kit for detecting nucleic acid of a monkey pox virus based on RAA-CRISPR / Cas12a and a detection method. The invention provides the combination of the specific RAA primer and the specific crRNA of the monkey pox virus, the RAA technology is combined with a CRISPR-Cas12a system, the visual rapid detection of nucleic acid of the monkey pox virus is realized, and compared with the traditional method, the method has the advantages of high specificity, high sensitivity, short detection time, accurate result and the like.

The invention discloses a method for detecting high sensitivity of monkey pox virus by a one-pot method RPA-CRISPR based on temperature control, and the method comprises the following steps: establishing a temperature-controlled RPA and CRISPR-Cas12b combined detection method, which is called as CRATS for short, by utilizing the reaction temperature difference between RPA amplification and CRISPR-Cas12b cutting; the reaction process in a tube system is regulated and controlled by stages through a temperature switching strategy: target amplification is completed at 37 DEG C firstly, then the temperature is raised to 60 DEG C, Cas12b cutting is started, so that sequential separation of amplification and cutting reactions is realized, and finally detection is realized through a fluorescence signal released by molecular beacon breakage. The problem that Cas enzyme cutting activity interferes with the amplification process in a traditional one-pot method reaction is solved, the detection sensitivity and specificity are improved, and a new technical means is provided for on-site rapid detection of the monkey pox virus.

The invention relates to a multi-antigen monkey pox RNA vaccine and a preparation method thereof, and relates to the technical field of nucleic acid vaccines. The multi-antigen mRNA comprises a sequence encoding an antigenic polypeptide of the monkey pox virus or an antigenic fragment, variant or derivative thereof, the antigenic polypeptide or the antigenic fragment thereof comprising at least viral proteins of an intracellular mature virus and an extracellular envelope virus of the monkey pox virus. The invention provides a multi-antigen mRNA vaccine aiming at monkey pox viruses and a sequence, mRNA can express antigen protein in in-vitro cells, high-titer anti-monkey pox virus A29L, A33L, B6R, M1R and E8L protein IgG can be detected after a mouse is immunized, a high-titer anti-vaccinia Tian Tan strain live virus neutralizing antibody is generated, good immunogenicity is achieved, and the vaccine can be used for preparing the multi-antigen mRNA vaccine aiming at the monkey pox viruses. The important significance is realized on the prevention of the monkey pox virus.

The invention provides a monkey pox virus nucleic acid detection kit and a preparation method thereof. The kit is used for qualitatively detecting monkey pox virus nucleic acid in vitro. Specific primers and probes are designed for the target F3L gene and the internal standard RNase P gene respectively, and the primer and probe combination is excellent in specificity, stability, compatibility, anti-interference performance and instrument adaptability, high in accuracy and sensitivity, easy to operate and high in detection speed. According to the kit disclosed by the invention, a freeze-drying reagent is prepared by utilizing the primer probe, so that the kit can completely get rid of the limitation of a cold chain, convenience is provided for remote areas lacking cold chain transportation and long-distance transportation, and the transportation and storage cost is greatly reduced. The monkey pox virus nucleic acid detection kit is simple in preparation method, good in reproducibility and easy to realize large-scale production and clinical application.

The invention relates to the technical field of biological medicine, in particular to a monkey pox target based on D8L protein and application of the monkey pox target, and the amino acid sequence of the D8L protein is shown as SEQ ID NO.1. After the relevance of the D8L protein on the inhibition effect of the interferon conduction pathway is detected, the application of the D8L protein as a monkey pox disease drug target is provided, and an antagonist for preparing a drug for preventing or treating the monkey pox disease is provided on the basis of the drug target.

The invention relates to the technical field of medicines, in particular to application of aspiramycin and / or imiquimod in preparation of a medicine for treating VACV. Aspiramycin and imiquimod which are screened from medicines on the market have antiviral activity, and experimental data show that the Aspiramycin and the imiquimod have anti-VACV medicine activity. Aspiramycin and imiquimod (especially combined application) are used for preparing the medicine for treating VACV, so that the time and cost of preclinical research can be greatly reduced. As the action mechanism of the pox viruses is not unique to the VACV, the combined strategy has the potential to be expanded to infection treatment of other pox viruses (such as monkey pox virus MPXV) and even other DNA (Deoxyribose Nucleic Acid) viruses.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting monkey pox virus based on a double visual loop-mediated isothermal amplification method, and belongs to the technical field of virus detection. The LAMP primer group provided by the invention comprises a primer group for detecting a monkey pox virus J2R gene and a primer group for detecting a monkey pox virus F3L-F4L gene. The LAMP primer group provided by the invention is high in sensitivity and strong in specificity, the F3L-F4L gene and the J2R gene of the monkey pox virus are detected through double targets, the detection accuracy is improved, and the omission ratio is greatly reduced. The neutral red pH indicator dye is added before the reaction, the cover does not need to be opened, aerosol pollution is reduced, the color change before and after the reaction is obvious, the detection result can be rapidly judged, and the operation is simple. By adding the PCR enhancer, the reaction efficiency and sensitivity are remarkably improved.

The invention belongs to the technical field of biological detection, and particularly relates to a one-tube self-amplification system, kit and method for detecting monkey pox virus. The one-tube self-amplification system comprises a DF probe for detecting DNA of a monkey pox virus B6R gene and crRNA (Complementary Ribonucleic Acid); the nucleotide sequence of the DF probe is as shown in SEQ ID NO: 3, and the nucleotide sequence of the crRNA is as shown in SEQ ID NO: 4. The trans-cleavage activity of Cas12a and affinity difference between phi29 DNA polymerase and single-chain and double-chain states of a probe are utilized, so that the problem of substrate competition of two enzymes in a co-reaction system is effectively solved, and efficient synergy of a double-signal amplification system in the space-time dimension is realized. The monkey pox virus DNA ultrafast signal amplification can be achieved within 8 min, the lowest detection limit is 87.68 aM (52.8 copies / microliter), and by combining a virus nucleic acid thermal cracking method, sample entering and result obtaining can be achieved within 10 min.

The invention relates to a Nile red derivative with photodynamic-photothermal performance as well as preparation and application thereof, two Nile red derivatives (TPACN and TPAOMCN) are elaborately designed and synthesized, and the structural formula is as follows: # imgabs0. The Nile red derivative disclosed by the invention can generate a large amount of active oxygen and efficient photothermal conversion under the irradiation of 660nm laser; a strong photodynamic-photothermal treatment effect is shown, multi-drug-resistant bacterium infection can be resisted to promote wound repair, and propagation of monkey pox viruses can be blocked. The TPAOMCN NFs provided by the invention can effectively remove drug-resistant bacteria in mouse wounds under laser irradiation, regulate inflammatory response and promote healing of infected wounds. In simulation of characteristic impetigo of monkey pox, after TPAOMCN NFs and laser treatment, virus titer in lesion is significantly reduced, infected lesion tissue is rapidly repaired, transmission of viruses to healthy mice is effectively blocked, and the TPAOMCN NFs has a huge application prospect.

The invention relates to the technical field of nucleic acid detection, in particular to an LAMP-CRISPR visual detection method for identifying a monkey pox virus based on an ATI gene and a kit and application of the LAMP-CRISPR visual detection method. Preparing an LAMP system and a CRISPR system, wherein the LAMP system and the CRISPR system are separated through mineral oil and air; carrying out LAMP amplification reaction to obtain an amplification product; adding an amplification product into a CRISPR (clustered regularly interspaced short palindromic repeats) system for enzyme digestion and visualization; wherein the reaction container comprises a PCR (Polymerase Chain Reaction) tube with the capacity of 200 mu L; the thickness of the mineral oil is 2.5-3mm; the air thickness ranges from 11 mm to 12 mm; the LAMP system is prepared at the bottom of the reaction container, the mineral oil is positioned on the upper surface of the LAMP system, and the CRISPR system is prepared in a circular groove of a tube cover of the PCR reaction tube. According to the detection method, a reaction system for visual detection is designed aiming at a monkey pox virus specific detection target, and an LAMP amplification system and a CRISPR detection system which are integrated into one tube are separated by utilizing mineral oil and liquid surface tension, so that not only is the detection convenience improved, but also the influence of high temperature during LAMP amplification on enzyme of the CRISPR detection system can be avoided.

The invention relates to a humanized monoclonal antibody aiming at monkey pox virus and application of the humanized monoclonal antibody, human monkey pox virus antibodies B6R-16A1, B6R-21G7, B6R-22D12, B6R-22F9 and B6R-22H1 with high neutralization activity are obtained by screening memory B cells specifically combined with anti-B6R of a human antibody humanized mouse, and the human monkey pox virus antibodies have high neutralization activity. The monkey pox virus vaccine has the characteristics of strong combining capacity with monkey pox virus, good in-vitro neutrality, large affinity and in-vivo protection effect.

The invention discloses a monoclonal antibody MPXV-mAb 975 and an application of the monoclonal antibody MPXV-mAb 981 in preparation of a medicine for preventing and treating a monkey pox virus. According to the invention, an established plaque phenotype experiment is utilized to evaluate the neutralizing activity of the two antibodies to the VACV, and the result shows that the two monoclonal antibodies can inhibit the propagation of the VACV between cells; effects of ADCC and ADCP mediated by the two antibodies are detected, and through a reporter gene experiment, the two antibodies show potential ADCC and ADCP activity; animal model data shows that both the two antibodies can protect CAST / EiJ mice and rhesus monkeys, monkey pox virus loads in different tissues and organs of the mice are remarkably reduced, and the number of acnes on the skin of the rhesus monkeys is reduced. The invention proves that the two monoclonal antibodies have in-vivo and in-vitro protection effects, can be used as effective prevention and treatment medicines for monkey pox virus infection, and provide candidate antibody preparations for clinical treatment of monkey pox virus infection.

The invention discloses an intelligent video analysis system and method for monkey pox infected skin based on deep learning, and relates to the technical field of image processing, and the system comprises an input end which is used for obtaining to-be-detected video sequence data; the processing unit is used for inputting the video sequence data to be detected into an improved three-dimensional convolutional neural network for processing to obtain a video sequence analysis result; the output end is used for displaying the dynamic evolution process, the infection position and the infection range of the monkey pox infection area and generating clinical analysis suggestions. According to the invention, the accuracy and efficiency of monkey pox infection detection are remarkably improved, and the kit has a relatively high clinical application value.

Based on a monkey pox virus immunodiagnosis technology based on up-conversion and long-afterglow nanoparticles, the preparation method comprises the following steps: preparing the long-afterglow and up-conversion nanoparticles by adopting a hydrothermal method and a solvothermal method respectively, and constructing the antibody-modified UCNP and PLNP nano probe by utilizing an EDC-NHS cross-linking system after carboxylation modification. The two nanoprobes and an antigen form an immune complex in a double antibody sandwich mode, the UCNPs is excited to emit light at the wavelength of 980 nm, the UCNPs and the PLNPs generate an LRET effect, an obvious signal peak is detected at the wavelength of 700 nm, and the fluorescence signal intensity is in direct proportion to the antigen content. The detection technology is portable and high in sensitivity, the detection limit is as low as the pg / ml level, and the monkey pox virus antigen standard substance can be detected in the range of 1 mg / ml to 1 ng / ml. According to the immunodetection technology provided by the invention, the detection of various pathogens can be realized by replacing antibody combinations aiming at different antigen epitopes, and the application potential in the aspect of infectious disease diagnosis is huge.

The invention discloses a suction type bionic nano particle for monkey pox prevention and a preparation method and application of the suction type bionic nano particle. According to the suction type bionic nano particles, lipid simulating lung surface active substances and macrophage membranes are mixed to form a carrier of the nano particles, monkey pox antigens and cGAMP are wrapped, the suction type bionic nano particles are synthesized, and the suction type bionic nano particles can effectively enhance humoral immunity and cellular immunity of anti-monkey pox viruses. The invention also provides an application of the inhalation type bionic nanoparticle in preparation of a nasal inhalation type vaccine preparation, and the nasal inhalation type vaccine preparation can induce mucosal immunity in lungs and mucosal tissues, effectively protect orthopoxvirus from invading through vagina and enteric cavity, and prevent sexual transmission of the orthopoxvirus; the nasal inhalation type vaccine can completely prevent mice from death caused by infection of attenuated strains of orthopoxvirus VACV by immunizing, rash is completely eliminated, and the nasal inhalation type vaccine has significance on effective prevention of monkey pox.

The invention discloses a monkey pox virus B6-A29 and A35-M1 fusion antigen and application thereof, and belongs to the technical field of biological medicine. The B6-A29 and A35-M1 fusion protein with good stability and uniform quality is designed, the neutralizing antibody activity is remarkably enhanced, the in-vivo protection effect is good, and the long-term protection effect is achieved. Compared with simple superposition of a single protein mixing effect, the method has better humoral immunity and cellular immunity, and the production, purification and quality control processes are simpler, more stable and lower in cost compared with a scheme of respectively producing four independent proteins and then mixing, and more accord with the requirements of vaccine industrial production.

The invention relates to a monkey pox virus vaccine based on a chimpanzee adenovirus vector, the chimpanzee adenovirus vector expresses a monkey pox virus immunizing antigen, and the monkey pox virus immunizing antigen comprises one or more of A35R, M1R and A29L. The monkey pox virus vaccine provided by the invention has the advantages of good immune effect, strong stability and the like.

The invention discloses a composition, a detection kit and a detection method for detecting monkey pox virus. The composition is composed of a primer pair composed of a single-stranded DNA as shown in a sequence 2 and a single-stranded DNA primer B as shown in a sequence 3, and a probe as shown in a sequence 4, 5'end nucleotide of the probe sequence is labeled with a fluorophore, and 3 'end nucleotide of the probe sequence is labeled with a quenching group. Based on the composition, a digital PCR (Polymerase Chain Reaction) system can be used for realizing specific detection on the mpox virus, the sensitivity is 10 times higher than that of the existing RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method, the operation is simple, and the composition can be applied to preparation of a kit for rapidly and efficiently detecting the mpox virus in a clinical sample, and is applied to screening and detecting the mpox virus.

The present invention relates to the use of raltitrexed in the alleviation and / or treatment of poxvirus infection. In view of the strong inhibition effect of raltitrexed on pox viruses including monkey pox viruses and vaccinia viruses, raltitrexed is very likely to become a specific medicine for diseases caused by infection of the pox viruses including the monkey pox viruses and the vaccinia viruses. The inventor considers that raltitrexed as a potential medicine for treating diseases caused by infection of poxviruses including monkey poxviruses and vaccinia viruses has great medicinal value, and is a promising candidate medicine for treating diseases caused by infection of poxviruses including monkey poxviruses and vaccinia viruses.

The invention relates to a primer and probe combination, a kit, a detection method and application. The primer sequence of the primer and probe combination is as follows: a forward primer: 5 '-ACATCTATTATAGCATAGC-3'; the invention relates to a monkey pox virus detection kit, which comprises a primer 1, a primer 2, a primer 3, a primer 4, a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5' and a probe 5 '.

The invention belongs to the technical field of image recognition, and discloses a monkey pox virus classification method and system based on image comparison and a storage medium, and the method comprises an individual reference construction module which is used for collecting normal skin and initial lesion images of the uninjured side of a patient, and generating a healthy texture template and a lesion initial reference library; performing gray normalization on the health texture template and the focus initial reference library through adaptive histogram stretching, and outputting a comparison reference image group; the symptom density quantification module is used for acquiring a current monitoring image of a patient, segmenting a focus area by combining and comparing the reference image group, extracting layered features of the focus area to generate a three-dimensional symptom density vector, extracting texture roughness of the surface of the focus area, and forming a focus quantification data set; the accuracy of disease monitoring is improved, and the clinical nursing process is optimized.

The invention belongs to the technical field of analysis and testing, and particularly discloses a monkey pox virus instant detection method based on combination of a resonance SERS probe and a test strip, and the method comprises the following steps: preparation of an Au NPs (at) IR808 (at) Ab1 resonance SERS probe, preparation of a biotin antibody Ab2 (at) Bio, preparation of an SA test strip, SERS signal sensing of monkey pox virus protein, and SERS qualitative and quantitative analysis. The sensitivity is improved by solving the root problem of probe sensitivity, the reaction of a double-antibody sandwich method in a test strip system is easily interfered by a matrix and the relationship between the binding efficiency and the sensitivity is researched, and by means of the external force of a biotin-streptavidin system which is higher in reaction rate and binding efficiency, the sensitivity of the biotin-streptavidin system can be detected, so that the sensitivity of the biotin-streptavidin system can be detected, and the sensitivity of the biotin-streptavidin system is improved. The efficiency of capturing the antibody probe by the paper strip is enhanced, so that the sensitivity is improved.