52 results about "Monkey Pox" patented technology

The invention discloses an anti-monkey pox virus antibody as well as a preparation method and application thereof. The invention specifically discloses eight kinds of antibodies (A35R-9E4, A35R-9F8, A35R-10C4, A35R-10D5, A35R-10E7, A35R-10H6, A35R-18E2 and A35R-18H2) which have very good affinity to monkey pox virus A35R protein, and the eight kinds of antibodies have very good neutralizing activity and a certain in-vivo protection effect.

The invention relates to the technical field of medicines, in particular to application of salinomycin in preparation of oral medicines for preventing or treating monkey pox virus (MPXV) infection. Salinomycin is a polyether antibiotic produced by actinomycetes. It is found for the first time that salinomycin can efficiently inhibit monkey pox virus infection on the cell culture level, and can significantly improve the survival rate of mice infected with monkey pox virus in small animal living bodies. More importantly, salinomycin can be administered by oral administration, has good bioavailability and safety, and provides a brand new treatment means for monkey pox virus infection. The medicinal value of salinomycin in resistance to monkey pox virus infection is reported for the first time at home and abroad, and the application prospect of salinomycin in the field of viral disease treatment is expanded.

The invention discloses a method for detecting high sensitivity of monkey pox virus by a one-pot method RPA-CRISPR based on temperature control, and the method comprises the following steps: establishing a temperature-controlled RPA and CRISPR-Cas12b combined detection method, which is called as CRATS for short, by utilizing the reaction temperature difference between RPA amplification and CRISPR-Cas12b cutting; the reaction process in a tube system is regulated and controlled by stages through a temperature switching strategy: target amplification is completed at 37 DEG C firstly, then the temperature is raised to 60 DEG C, Cas12b cutting is started, so that sequential separation of amplification and cutting reactions is realized, and finally detection is realized through a fluorescence signal released by molecular beacon breakage. The problem that Cas enzyme cutting activity interferes with the amplification process in a traditional one-pot method reaction is solved, the detection sensitivity and specificity are improved, and a new technical means is provided for on-site rapid detection of the monkey pox virus.

The invention relates to the technical field of medicines, in particular to application of aspiramycin and / or imiquimod in preparation of a medicine for treating VACV. Aspiramycin and imiquimod which are screened from medicines on the market have antiviral activity, and experimental data show that the Aspiramycin and the imiquimod have anti-VACV medicine activity. Aspiramycin and imiquimod (especially combined application) are used for preparing the medicine for treating VACV, so that the time and cost of preclinical research can be greatly reduced. As the action mechanism of the pox viruses is not unique to the VACV, the combined strategy has the potential to be expanded to infection treatment of other pox viruses (such as monkey pox virus MPXV) and even other DNA (Deoxyribose Nucleic Acid) viruses.

The invention belongs to the technical field of biological detection, and particularly relates to a one-tube self-amplification system, kit and method for detecting monkey pox virus. The one-tube self-amplification system comprises a DF probe for detecting DNA of a monkey pox virus B6R gene and crRNA (Complementary Ribonucleic Acid); the nucleotide sequence of the DF probe is as shown in SEQ ID NO: 3, and the nucleotide sequence of the crRNA is as shown in SEQ ID NO: 4. The trans-cleavage activity of Cas12a and affinity difference between phi29 DNA polymerase and single-chain and double-chain states of a probe are utilized, so that the problem of substrate competition of two enzymes in a co-reaction system is effectively solved, and efficient synergy of a double-signal amplification system in the space-time dimension is realized. The monkey pox virus DNA ultrafast signal amplification can be achieved within 8 min, the lowest detection limit is 87.68 aM (52.8 copies / microliter), and by combining a virus nucleic acid thermal cracking method, sample entering and result obtaining can be achieved within 10 min.

The invention relates to a humanized monoclonal antibody aiming at monkey pox virus and application of the humanized monoclonal antibody, human monkey pox virus antibodies B6R-16A1, B6R-21G7, B6R-22D12, B6R-22F9 and B6R-22H1 with high neutralization activity are obtained by screening memory B cells specifically combined with anti-B6R of a human antibody humanized mouse, and the human monkey pox virus antibodies have high neutralization activity. The monkey pox virus vaccine has the characteristics of strong combining capacity with monkey pox virus, good in-vitro neutrality, large affinity and in-vivo protection effect.

The invention discloses a monoclonal antibody MPXV-mAb 975 and an application of the monoclonal antibody MPXV-mAb 981 in preparation of a medicine for preventing and treating a monkey pox virus. According to the invention, an established plaque phenotype experiment is utilized to evaluate the neutralizing activity of the two antibodies to the VACV, and the result shows that the two monoclonal antibodies can inhibit the propagation of the VACV between cells; effects of ADCC and ADCP mediated by the two antibodies are detected, and through a reporter gene experiment, the two antibodies show potential ADCC and ADCP activity; animal model data shows that both the two antibodies can protect CAST / EiJ mice and rhesus monkeys, monkey pox virus loads in different tissues and organs of the mice are remarkably reduced, and the number of acnes on the skin of the rhesus monkeys is reduced. The invention proves that the two monoclonal antibodies have in-vivo and in-vitro protection effects, can be used as effective prevention and treatment medicines for monkey pox virus infection, and provide candidate antibody preparations for clinical treatment of monkey pox virus infection.

The invention discloses an intelligent video analysis system and method for monkey pox infected skin based on deep learning, and relates to the technical field of image processing, and the system comprises an input end which is used for obtaining to-be-detected video sequence data; the processing unit is used for inputting the video sequence data to be detected into an improved three-dimensional convolutional neural network for processing to obtain a video sequence analysis result; the output end is used for displaying the dynamic evolution process, the infection position and the infection range of the monkey pox infection area and generating clinical analysis suggestions. According to the invention, the accuracy and efficiency of monkey pox infection detection are remarkably improved, and the kit has a relatively high clinical application value.

Based on a monkey pox virus immunodiagnosis technology based on up-conversion and long-afterglow nanoparticles, the preparation method comprises the following steps: preparing the long-afterglow and up-conversion nanoparticles by adopting a hydrothermal method and a solvothermal method respectively, and constructing the antibody-modified UCNP and PLNP nano probe by utilizing an EDC-NHS cross-linking system after carboxylation modification. The two nanoprobes and an antigen form an immune complex in a double antibody sandwich mode, the UCNPs is excited to emit light at the wavelength of 980 nm, the UCNPs and the PLNPs generate an LRET effect, an obvious signal peak is detected at the wavelength of 700 nm, and the fluorescence signal intensity is in direct proportion to the antigen content. The detection technology is portable and high in sensitivity, the detection limit is as low as the pg / ml level, and the monkey pox virus antigen standard substance can be detected in the range of 1 mg / ml to 1 ng / ml. According to the immunodetection technology provided by the invention, the detection of various pathogens can be realized by replacing antibody combinations aiming at different antigen epitopes, and the application potential in the aspect of infectious disease diagnosis is huge.

The invention discloses a monkey pox virus B6-A29 and A35-M1 fusion antigen and application thereof, and belongs to the technical field of biological medicine. The B6-A29 and A35-M1 fusion protein with good stability and uniform quality is designed, the neutralizing antibody activity is remarkably enhanced, the in-vivo protection effect is good, and the long-term protection effect is achieved. Compared with simple superposition of a single protein mixing effect, the method has better humoral immunity and cellular immunity, and the production, purification and quality control processes are simpler, more stable and lower in cost compared with a scheme of respectively producing four independent proteins and then mixing, and more accord with the requirements of vaccine industrial production.

The invention relates to a monkey pox virus vaccine based on a chimpanzee adenovirus vector, the chimpanzee adenovirus vector expresses a monkey pox virus immunizing antigen, and the monkey pox virus immunizing antigen comprises one or more of A35R, M1R and A29L. The monkey pox virus vaccine provided by the invention has the advantages of good immune effect, strong stability and the like.

The invention discloses a composition, a detection kit and a detection method for detecting monkey pox virus. The composition is composed of a primer pair composed of a single-stranded DNA as shown in a sequence 2 and a single-stranded DNA primer B as shown in a sequence 3, and a probe as shown in a sequence 4, 5'end nucleotide of the probe sequence is labeled with a fluorophore, and 3 'end nucleotide of the probe sequence is labeled with a quenching group. Based on the composition, a digital PCR (Polymerase Chain Reaction) system can be used for realizing specific detection on the mpox virus, the sensitivity is 10 times higher than that of the existing RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method, the operation is simple, and the composition can be applied to preparation of a kit for rapidly and efficiently detecting the mpox virus in a clinical sample, and is applied to screening and detecting the mpox virus.

The invention relates to a primer and probe combination, a kit, a detection method and application. The primer sequence of the primer and probe combination is as follows: a forward primer: 5 '-ACATCTATTATAGCATAGC-3'; the invention relates to a monkey pox virus detection kit, which comprises a primer 1, a primer 2, a primer 3, a primer 4, a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5', a primer 5 ', a primer 5' and a probe 5 '.

The invention belongs to the technical field of image recognition, and discloses a monkey pox virus classification method and system based on image comparison and a storage medium, and the method comprises an individual reference construction module which is used for collecting normal skin and initial lesion images of the uninjured side of a patient, and generating a healthy texture template and a lesion initial reference library; performing gray normalization on the health texture template and the focus initial reference library through adaptive histogram stretching, and outputting a comparison reference image group; the symptom density quantification module is used for acquiring a current monitoring image of a patient, segmenting a focus area by combining and comparing the reference image group, extracting layered features of the focus area to generate a three-dimensional symptom density vector, extracting texture roughness of the surface of the focus area, and forming a focus quantification data set; the accuracy of disease monitoring is improved, and the clinical nursing process is optimized.

The invention relates to application of SLS in preparation of a product capable of improving LAMP detection performance, and belongs to the technical field of biology. The invention provides an application of sodium lauroyl sarcosinate (SLS) in preparation of a product capable of improving LAMP (loop-mediated isothermal amplification) detection performance. On one hand, the SLS has a target specificity optimization effect on detection of targets such as the monkey pox virus in an LAMP reaction system, and by adjusting the ion environment of the LAMP reaction system, the SLS can improve the specific amplification efficiency of the monkey pox virus target by 20% or above while completely inhibiting non-specific amplification; on the other hand, the SLS has the effect of improving the sensitivity and the detection rate on detection of targets such as the monkey pox virus in an LAMP reaction system, and under the low template concentration, the SLS can improve the detection rate of the monkey pox virus targets from 60% to 100% by inhibiting formation of primer dimers. Therefore, the SLS has a promising application prospect in LAMP-based nucleic acid detection.

The invention discloses a monkey pox virus typing nucleic acid detection primer probe combination, a kit and application, and belongs to the technical field of biology. The primer probe combination comprises six groups of specific primer pairs and six corresponding probes, and the six groups of specific primer pairs and the six corresponding probes are respectively used for accurately identifying the monkey pox viruses such as Clade Ia, Clade Ib, Clade II, Clade IIb A.1, Clade IIb B.1 and Clade IIb C.1; the primer probe combination provided by the invention has the advantages of high specificity, high sensitivity and accurate typing, and effectively overcomes the influence of target sequence deletion or mutation caused by virus variation on the detection accuracy through combination of locked nucleic acid modified probes and a multiplex fluorescent PCR technology; the method can realize rapid and parallel identification of main branches and epidemic pedigree of the monkey pox virus, and provides reliable technical support for epidemic prevention and control and clinical diagnosis.

The invention belongs to the field of medicines, and particularly discloses application of a perfoliote knotweed herb water extract in preventing and treating herpes virus and poxvirus infection. The extraction method of the perfoliote knotweed herb water extract comprises the following steps: S1, picking a perfoliote knotweed herb fresh product, cutting into sections, and crushing with a crusher; s2, performing ultrasonic extraction with deionized water at the normal temperature of 20-45 DEG C; s3, filtering with filter cloth to obtain a perfoliote knotweed herb water extract; and S4, standing the perfoliote knotweed herb water extract for 12-24 hours, and centrifuging to obtain water extract supernate. The perfoliote knotweed herb water extract obtained by the invention has inhibitory activity on poxviridae orthopoxvirus vaccinia virus infection, and can be used for treating pox, monkey pox and the like; the compound has inhibitory activity on herpes simplex virus type 1 and varicella-zoster virus infection of herpes viridae, and can be used for treating herpes simplex virus infection of oral cavity, periorbital and pudendum skin or mucosa and treating varicella and zoster. The method has application value in practice.

The invention discloses a method for establishing immunochromatography for detecting monkey pox virus based on a MoS2 (at) Au nanosheet. The method comprises the following steps: 1, sequentially adsorbing a Raman report molecule and a monkey pox virus specific antibody on the surface of a MoS2 (at) Au nanosheet to prepare a nanoprobe, and dispersing the nanoprobe in PBST to obtain a nanoprobe dispersion liquid; 2, preparing an immunochromatography test strip; 3, mixing a to-be-detected sample with the nanoprobe to obtain a to-be-detected solution, and applying the to-be-detected solution to the immunochromatography test strip for a chromatographic reaction; and 4, after the chromatographic reaction is completed, signal acquisition is performed on the detection line on the immunochromatographic test strip, and an adopted signal reading mode comprises at least one of visual colorimetry, surface enhanced Raman scattering and photo-thermal detection. According to the present invention, the multi-mode detection can be achieved, the sensitivity is high, and the detection flexibility and the result reliability are substantially enhanced through the design, such that the rapid screening requirement of the base site can be met, and the accurate quantification requirement of the laboratory can be met.

The invention provides application of a milk-derived active component in preparation of an orthopoxvirus inhibitor. The milk-derived active component comprises one or more of lactoferrin, whey protein, milk fat globule membrane and osteopontin. The milk-derived active component has the activity of remarkably inhibiting orthopox virus infection, and is a potential preparation for treating monkey pox and vaccinia virus infectious diseases. The lactoferrin can inhibit virus infection in the whole life cycle of the virus, has important medicinal value, and is a promising candidate preparation for treating infectious diseases of monkey pox virus and other orthopox virus.

The invention relates to a specific monoclonal antibody and application thereof in detection and neutralization of monkey pox virus. The amino acid sequence of a light chain CDR1 of the monoclonal antibody is as shown in SEQ ID No.1, the amino acid sequence of CDR2 of the monoclonal antibody is as shown in SEQ ID No.2, and the amino acid sequence of CDR3 of the monoclonal antibody is as shown in SEQ ID No.3; the amino acid sequence of a heavy chain CDR1 of the monoclonal antibody is as shown in SEQ ID No.4, the amino acid sequence of CDR2 of the monoclonal antibody is as shown in SEQ ID No.5, and the amino acid sequence of CDR3 of the monoclonal antibody is as shown in SEQ ID No.6. The recombinant monkey pox virus surface protein A35R is used as an antigen, the monoclonal antibody obtained through screening has good affinity with the recombinant monkey pox virus surface protein A35R at the molecular level and the cellular level, the infection efficiency of a VACV replication defective strain in VERO cells can be remarkably inhibited, and a good monkey pox virus in-vitro neutralization effect is achieved.

The invention relates to a monkey pox virus monoclonal antibody and a preparation method and application thereof.The monkey pox virus monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises a heavy chain VHCDR1, a heavy chain VHCDR2 and a heavy chain VHCDR3, and the amino acid sequences of the heavy chains VHCDR1, VHCDR2 and VHCDR3 are shown as SEQ ID NO.1-3; the light chain variable region comprises a light chain VLCDR1, a light chain VLCDR2 and a light chain VLCDR3, and the amino acid sequences of the light chains VLCDR1, VLCDR2 and VLCDR3 are as shown in SEQ ID NO. The monkey pox virus specific recombinant protein antigen provided by the invention only contains the specific antigen epitope of monkey pox virus A29L17-49 protein, can effectively distinguish monkey pox virus from other orthopox viruses, ensures that a monkey pox virus monoclonal antibody prepared by taking the monkey pox virus specific recombinant protein antigen as an immunogen only can specifically recognize the monkey pox virus, and improves the detection accuracy of the monkey pox virus.

The application provides an RNA, an RNA combination and application thereof, and a multivalent monkeypox vaccine, and relates to the technical field of biotechnology.The mRNA provided by the application is obtained after codon optimization, and can express antigen proteins in vitro cells with high efficiency; the mRNA combination provided by the application has high expression, and after immunizing mice, high-titer neutralizing antibodies against the live virus of the vaccinia virus Tianlu strain can be detected, and the mRNA combination has good immunogenicity, and is of great significance for the prevention of the monkeypox virus.The multivalent monkeypox mRNA vaccine provided by the application can realize stable and safe expression in vivo and effectively activate an immune response, and can cause a neutralizing antibody response, and in the serum of the immunized mice, high-titer neutralizing antibodies against the live virus of the vaccinia virus Tianlu strain can be detected.

The invention provides an mRNA-MV2 vaccine of monkey pox virus and a preparation method of the mRNA-MV2 vaccine, and relates to the technical field of vaccine preparation. The amino acid sequence of the mRNA-MV2 vaccine is as shown in SEQ ID NO.1, the nucleic acid sequence of the mRNA-MV2 vaccine is as shown in SEQ ID NO.2, and the vaccine is obtained by forming a single-chain dimer conformation through reasonable excision and reassembly and connecting two antigens of conservative A35R and conservative M1R in series by using a cutting sequence P2A. The invention overcomes the defects of the prior art, and designs and prepares a vaccine which has a good prevention effect on MPXV infection, and is safe, stable, short in research and development period and low in cost.

The invention discloses a monkey pox virus self-assembled nanoparticle and a preparation method and application thereof, and relates to the technical field of monkey pox virus vaccine research and development, the monkey pox virus nanoparticle (NP) is a DAM-NP nanoparticle vaccine formed by covalent coupling of a DAM antigen and SD-Ferritin, the DAM antigen comprises a fusion sequence of monkey pox virus M1 protein and A35 protein, the N end is connected with a GvTagOpti tag, the D-Ferritin is connected with a GvTagOpti tag, and the D-Ferritin is connected with a GvTagOpti tag. The middle parts are connected through a glycine-serine-glycine spacer region; the SD-Ferritin is formed by fusing an SdCatcher fragment and the N end of the helicobacter pylori ferritin, and the SdCatcher fragment and the N end of the helicobacter pylori ferritin are connected through a GSG spacer region; the coupling ratio of the DAM antigen to the SD-Ferritin is 3: 1, and covalent binding is formed after incubation in an assembly buffer solution at 4 DEG C for 24 hours. The monkey pox DAM-NP vaccine is constructed by fusing M1 and A35 antigens of the monkey pox virus in a bivalent form and displaying the M1 and A35 antigens on the surfaces of self-assembled 24-polymer ferritin nanoparticles by using a GvTagOpti / SdCatcher covalent coupling system, antigen-specific body fluid and cellular immune response can be stimulated, and protective immunity can be induced by a single dose of the monkey pox DAM-NP vaccine.

The present invention relates to the field of virus colorimetric quantification, and discloses a visual analysis-based monkey pox virus constant temperature occlusion detection quantitative method, which comprises: specifically amplifying a monkey pox virus target gene in a constant temperature state through a constant temperature nucleic acid amplification technology, and carrying out a chemical reaction treatment on the amplification product and a color developing reagent. An amplification product reaction solution after the chemical reaction is obtained for image analysis, a color-concentration change curve fitting model is constructed, a color characteristic value is output, and finally the output color characteristic value is converted into the quantitative concentration of the monkey pox virus. According to the method, traditional subjective colorimetric interpretation is converted into an objective and accurate quantitative result, and the accuracy and reliability of detection are remarkably improved; the advantages of rapidness and equipment simplification of isothermal amplification and the characteristics of intelligence and automation of visual analysis are fully utilized, the operation threshold and dependence on professional equipment are reduced, and the method is suitable for rapid quantitative detection and epidemic situation monitoring of the monkey pox virus.

The invention belongs to the technical field of monoclonal antibody preparation, and particularly relates to a monoclonal antibody targeting monkey pox virus A27L protein and application of the monoclonal antibody. Specifically, the hybridoma cell strains are constructed by taking the monkey pox virus A27L protein as an immunogen, and monoclonal antibodies secreted by the cell strains have strong binding specificity with the monkey pox virus A27L protein, do not have cross reaction with other proteins, can be used for distinguishing monkey pox virus from other viruses, and can be used for detecting the monkey pox virus. The kit is used for early rapid and accurate detection of the monkey pox virus.

The invention relates to an artificially synthesized recombinant monoclonal antibody taking a human antibody amino acid sequence as a framework. The monoclonal antibody H11 is obtained by screening from a Tomlinson I + J phage library by utilizing a phage display technology, and CDR1, CDR2 and CDR3 regions formed by the new amino acid sequences can be specifically combined with A29L protein. The kit shows the characteristics of good stability, high specificity, high affinity and the like, and provides a tool support for subsequent diagnosis and detection of the monkey pox virus taking A29L protein as a target spot.

The invention discloses a MoSe2 (at) Au / Pt composite probe integrating catalysis and photothermal effects and preparation and application thereof.The method comprises the steps that a tannic acid aqueous solution of MoSe2 is subjected to ultrasonication, centrifugation and washing and then dissolved in water, HAuCl4. 4H2O and H2PtCl6. 6H2O solutions are added for stirring, centrifugation is conducted, sediment is collected and washed, obtained MoSe2 (at) Au / Pt nanosheets are dissolved in a buffer solution, and the MoSe2 (at) Au / Pt composite probe is obtained. And adding a monkey pox virus labeled antibody, and sealing by using bovine serum albumin. The problems that an existing monkey pox antigen detection test strip is extremely low in clinical sensitivity and extremely high in false negative rate due to the fact that the existing monkey pox antigen detection test strip depends on a colloidal gold marker with limited signal intensity and a single naked eye colorimetric interpretation mode are solved, and due to the rapid charge separation efficiency and increased active sites of MoSe2 (at) Au / Pt, the clinical sensitivity of the monkey pox antigen detection test strip is greatly improved. The colorimetric-catalytic colorimetric-photo-thermal three-mode high-sensitivity detection on the MPXV is realized.