Preparation of carrageenan sulfated oligosaccharide with antivirus activity

Abstract

The invention relates to a method for preparing carrageenin-sulfate oligosaccharide with antiviral activity, which is characterized in that culture medium for beta-glucanase production is prepared, marine cellulophaga is inoculated; kappa-carrageenin crude digestive enzyme liquid is fermented and extracted; additionally, the kappa-carrageenin is dissolved in distilled water and undergoes enzymatic hydrolysis reaction after the kappa-carrageenin crude digestive enzyme liquid is added; the enzymatic hydrolysis reaction liquid is converted into concentrated liquid after the rotary evaporation; enzymatic hydrolysis oligosaccharide fragment is collected by precipitating organic solvent; and then sulfate modification is carried out on the enzymatic hydrolysis oligosaccharide fragment and carrageenin-sulfate oligosaccharide is obtained. The invention has the advantage that the step combining enzymatic hydrolysis and the sulfate modification is applied, so that the preparation process is simple, the product yield is high, the product quality is stable and active groups during the preparation are not damaged, in addition, the product has the antiviral activity, in particular to the obvious antiviral activity against herpes simplex virus.

Description

technical field [0001] The invention relates to a preparation method of carrageenan sulfate oligosaccharide with antiviral activity. Background technique [0002] At present, there have been reports on the activity of carrageenan sulfate oligosaccharides, including anti-virus, anti-tumor, anti-ulcer, anti-coagulation and other biological activities of carrageenan sulfate oligosaccharides, especially for HIV-1, HIV-2, herpes Enveloped viruses such as viruses have broad-spectrum inhibitory activity. The antiviral activity of carrageenan sulfated oligosaccharides is closely related to the molecular weight, the degree of sulfation and the spatial configuration. High molecular weight carrageenan is difficult to be absorbed by the body due to its high viscosity and difficulty in diffusion. However, the carrageenan obtained after proper degradation Sulfated oligosaccharides are easy to absorb, and their toxicity is greatly reduced. Most of the preparation methods of carrageenan s...

AI Technical Summary

Problems solved by technology

Most of the preparation methods of carrageenan sulfate oligosaccharides are based on chemical methods, but chemical methods have the disadvantages of difficult control of reaction conditions, poor reaction specificity, complex product composition and difficult separation of target products, especially in the process of chemical degradation. The functional group of the target product will inevitably be destroyed

At present, the reports of enzymatic hydrolysis to obtain biologically active carrageenan sulfated oligosaccharides include: a preparation method of galactan sulfate with anti-tumor activity (Chinese Patent Application No. 03138975.9), a carrageenan with antioxidant activity Preparation method of gum oligosaccharide sulfate ester (Chinese Patent Application No. 200610070792.5), but there is no report about the preparation of carrageenan sulfate oligosaccharide with antiviral activity by enzymatic hydrolysis