Porcine alpha interferon gene and synthetic method thereof

Abstract

The invention discloses a porcine alpha interferon gene and a synthetic method thereof. The gene provided by the invention has the advantages that the high expression of the interferon in pichia pastoris is realized; porcine alpha interferon protein with antiviral activity is acquired; the anti-VSV activity of the porcine alpha interferon gene prepared from the synthetic method is (3.82*107) U / mg. The synthetic method designs the coding nucleotide sequence of the porcine alpha interferon on the basis that the amino acid sequence of the porcine alpha interferon is not changed; each codon in a reading frame is preferred by pichia pastoris; the coding nucleic acid is designed in the synthetic steps; a proper expression vector is inserted in the synthetic nucleic acid to build recombinant expression vector; finally the built recombinant expression vector converts proper pichia pastoris for inducible expression; the result shows the high expression of the porcine alpha interferon in the pichia pastoris, and the porcine alpha interferon protein with antiviral activity is acquired; the synthetic method is suitable for the synthesis of the porcine alpha interferon.

Description

technical field [0001] The invention belongs to the field of genetic engineering and relates to a gene and a synthesis method thereof, in particular to a porcine alpha interferon gene and a synthesis method thereof. Background technique [0002] Interferon is mainly produced by animal cells under the action of inducers such as viruses. Many animals, including humans, mammals, birds, fish, etc., can produce interferon. According to the antigenic properties and molecular structure of interferon, it is divided into different types. Interferon (IFN) is divided into type I and type II. Type I interferon gene lacks introns, including 5 types of α, β, δ, ω, and τ. Type II has only one type γ, which contains introns . They have related structures and share the same receptor (primary receptor), which is widely distributed, including monocytes / macrophages, polymorphonuclear leukocytes, B cells, T cells, platelets, epithelial cells, endothelial cells cells and tumor cells. Express...

AI Technical Summary

Problems solved by technology

The natural porcine IFN-α induced by inducers is expensive due to many reasons such as few sources, high cost, and complicated purification process, which greatly limits the clinical and scientific applications. System, so that it can be efficiently expressed, and the production of interferon with the advantages of low cost, broad-spectrum antiviral activity, non-toxicity, and no residue is undoubtedly an effective way to promote the application of interferon in veterinary clinics

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