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Application of exogenous ATG10S protein in preparation of antiviral drugs

An ATG10S, protein technology, applied in the field of medicine and biology, can solve problems such as no ATG10S research report, and achieve the effect of activating autophagy

Pending Publication Date: 2021-05-14
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no research report on ATG10S

Method used

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  • Application of exogenous ATG10S protein in preparation of antiviral drugs
  • Application of exogenous ATG10S protein in preparation of antiviral drugs
  • Application of exogenous ATG10S protein in preparation of antiviral drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1. The rhATG10S protein expressed in Escherichia coli degrades viral proteins by activating the autophagy activity of cells

[0107] The inventor's previous research has proved that the endogenous ATG10S protein of cells can degrade HCV viral proteins by promoting the formation of autophagy lysosomes 2-4 .

[0108] In the present invention, we examined whether the rhATG10S protein heterologously expressed in E. coli also functions as the endogenous protein.

[0109] In the experiment, the inventors tested cells containing 11 viral proteins of 5 viruses (HBV-X, HCV-CORE / NS5B, HIV-RT, ZIKA-NS5, SARS-CoV2-S / N / M / NSP5 / NSP9 / NSP12) (each HepG2 cell transfers an exogenous protein, and 11 kinds of HepG2 cells transformed with different exogenous viral proteins were tested.) The rhATG10S protein expressed by Escherichia coli was added in the culture medium, and the viral protein and Levels of autophagic flux-associated proteins.

[0110] The results showed that after ...

Embodiment 2

[0112] Example 2, ATG10S expressed in Escherichia coli can enter the nucleus to activate the expression of type III interferon family genes

[0113] The inventor's previous research has confirmed that:

[0114] (1) In cells containing HCV, endogenous ATG10S protein can enter the nucleus as a new transcription factor, activate the promoter of type III interferon-2 (IFNL2) gene, and promote the high expression of IFNL2 2 ;

[0115] (2) IFNL2 protein can directly recognize and promote the fusion of autophagosomes and lysosomes, and enhance the activity of autophagy to degrade viral proteins;

[0116] (3) Down-regulation of IFNL2 can prevent the formation of autolysosomes and viral degradation 2,4 .

[0117] In order to further understand the anti-virus mechanism of rhATG10S derived from heterologous cells, in the present invention, we detected whether rhATG10S protein can also enter cells and related subcellular localization regions from extracellular.

[0118] 1. Add the rhA...

Embodiment 3

[0121] Embodiment 3, ATG10S antiviral mechanism

[0122] In order to further understand the antiviral mechanism of ATG10S, we tested the interaction between ATG10S and IFNL2 protein and autophagy flux protein by Co-IP experiment based on HCV subreplicon cell model. The results show,

[0123] 1. Compared with the model group and model group + ATG10, ATG10S can significantly enhance autophagic flux and increase IFNL2 protein levels; when immunoprecipitated with LAMP2 (a lysosomal membrane protein) antibody, it was found that ATG10S promoted lysosome Binding to IFNL2, P62, and LC3B (autophagophore marker), ATG10S was found to promote its binding to lysosomes and IFNL2 proteins when immunoprecipitated with antibodies to flag (here, flag-ATG10 and flag-ATG10S proteins) . But ATG10 has no such function ( Figure 8 ) 1 .

[0124] 2. Using the HCV whole virion infection experiment, the anti-whole virus effect of IFNL2 was detected. Taking non-infected cells as the control, the i...

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Abstract

The invention relates to application of an exogenous ATG10S protein in preparation of an antiviral drug. The exogenous ATG10S is an rhATG10S protein, the coding nucleotide sequence of the exogenous ATG10S is shown as SEQ ID NO. 1 or SEQ ID NO. 2, and the amino acid sequence of the rhATG10S protein is shown as SEQ ID NO. 3. The invention also relates to application of the rhATG10S protein in preparation of drugs for inhibiting viruses. The invention further discloses a method for screening antiviral drugs by taking the ATG10S protein as a target spot.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically relates to the application of an exogenous rhATG10S protein in the preparation of antiviral drugs. Background technique [0002] Viral infections still occupy an important position in human infectious diseases, such as HIV, ZIKV, HBV, HCV, Ebola, influenza virus, encephalitis virus, etc. are still important pathogens affecting human health; especially the new coronary pneumonia virus (SARS-COV -2) The infection caused is very serious, and the treatment means for the virus is still very limited. [0003] In recent years, the research results of the correlation between autophagy mechanism and diseases have attracted great attention in the field of pharmaceutical research and development. There are many members of the autophagy gene family, which are involved in different stages of the autophagy process. Studies have shown that in addition to normal cell physiology su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/70G01N33/68A61K31/435A61K45/00A61P31/14A61P31/18A61P31/20C12R1/19
CPCC07K14/4705C12N15/70G01N33/68A61K45/00A61K31/435A61P31/18A61P31/14A61P31/20C12N2800/22G01N2500/04G01N2333/4706Y02A50/30
Inventor 张靖溥张苗青马媛媛陈博张锐
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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