Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of pseudolaric acid in preparation of medicine for preventing and/or treating malignant tumors

A technique for malignant tumor and hibiscus bark, which is applied to the application field of hibiscus bark propionic acid in the preparation of drugs for preventing and/or treating malignant tumors, and can solve the problems of unclear usage of Hibiscus bark propionic acid and unclear mechanism of action.

Active Publication Date: 2022-06-28
葛鹏飞
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use and mechanism of action of the natural small molecular compound hibiscus hibiscus propionic acid extracted from hibiscus bark is still unclear, especially hibiscus hibiscus propionic acid has not been reported in the prevention and / or treatment of malignant tumors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of pseudolaric acid in preparation of medicine for preventing and/or treating malignant tumors
  • Application of pseudolaric acid in preparation of medicine for preventing and/or treating malignant tumors
  • Application of pseudolaric acid in preparation of medicine for preventing and/or treating malignant tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Inhibitory effect of fenugreek propionic acid on the proliferation of human glioma cells

[0036] The human brain glioma cells U251, U87 and U118 were inoculated in DMEM medium in 96-well plates, and incubated with carbon dioxide overnight. , 50umol / L, 100umol / L, 200umol / L) after incubation for 48 hours, the MTT method was used to detect the cell proliferation ability, and the results were as follows figure 1 As shown in Table 1, the survival rates of U251 cells were 96.67%, 91.97%, 68.75%, 43.59% and 34.05%, respectively; figure 2 As shown in Table 2, the survival rates of U87 cells were 97.19%, 91.73%, 69.27%, 46.51% and 31.94%, respectively; image 3 As shown in Table 3, the survival rates of U118 cells were 98.36%, 93.04%, 67.57%, 44.42% and 34.60%, respectively.

[0037] The detection results showed that the survival rate of glioma cells decreased correspondingly with the increase of the concentration of propionic acid in hibiscus. It was confirmed th...

Embodiment 2

[0044]Example 2 Toxicity of fenugreek propionic acid to normal cells

[0045] In order to detect the toxic effect of hibiscus propionic acid on normal cells, in this experiment, human umbilical vein epithelial cells HUVEC were inoculated into DMEM medium in a 96-well plate, and incubated with carbon dioxide overnight in a carbon dioxide incubator. (12.5umol / L, 25umol / L, 50umol / L, 100umol / L, 200umol / L) after incubation for 48 hours, the cell proliferation ability was detected by MTT method, and the results were as follows Figure 4 As shown in Table 4, the survival rates of HUVEC cells were 100%, 99.15%, 95.84%, 89.80% and 82.42%, respectively.

[0046] The detection results showed that compared with the survival rate of human glioma cells U251, U87 and U118, the 25umol / L and 50umol / L hibiscus propionic acid were less toxic to human umbilical vein epithelial cells HUVEC, At the same time, it can effectively kill human glioma cells.

[0047] Table 4 Inhibitory effect of hibisc...

Embodiment 3

[0049] Example 3 Study on the mechanism of killing of human brain glioma cells by fenugreek propionic acid

[0050] (1) Activation of autophagic flux in human glioma cells by propionic acid

[0051] In order to elucidate the mechanism of hibiscus propionic acid on human glioma cells, in this experiment, 50umol / L hibiscus propionic acid was incubated with human glioma cells U87 for 6, 12, 24 and 48 h, and the protein was extracted. Then, Western blotting method was used to detect the expression of autophagy-related proteins, and the results were as follows Figure 5 As shown, hibiscus propionic acid can up-regulate the expression of protein ATG5 and protein LC3B II in a time-dependent manner, while correspondingly inhibiting the expression of autophagy substrate protein P62. The red-green tandem fluorescent LC3B protein particles were transfected into human brain glioma cells U87 by lentivirus, and it was further verified whether fenugreek propionic acid induced autophagy. Th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of pseudolaric acid in preparation of medicines for preventing and / or treating malignant tumors, and relates to the field of new application of medicines. The invention discloses a novel application of pseudolaric acid in preparation of medicines for preventing and / or treating malignant tumors. The malignant tumors comprise brain glioma, liver cancer, breast cancer and ovarian cancer; the concentration of the pseudolaric acid is 12.5 to 200 [mu] mol / L. Experiments prove that pseudolaric acid can effectively kill various malignant tumor cells such as human brain glioma cells, human liver cancer cells, human breast cancer cells and human ovarian cancer cells in a concentration-dependent manner, and has a certain dose-effect relationship; the pseudolaric acid has small toxicity to human normal cells, and can activate autophagic flux in human glioma cells, and the human glioma cells are killed by activating the autophagic flux in the human glioma cells. The invention develops new application of pseudolaric acid, and provides a new therapeutic scheme for treating various malignant tumor cells.

Description

technical field [0001] The invention relates to the technical field of new uses of medicine, in particular to the application of hibiscus propionic acid in the preparation of medicines for preventing and / or treating malignant tumors. Background technique [0002] Cancer is collectively referred to as malignant tumor in medicine. It is a complex and major disease that seriously threatens human health. It is the second leading cause of human death in the world after cardiovascular and cerebrovascular diseases. [0003] Human brain gliomas are the most common primary malignant tumors in the brain, which can be found in all age groups, mostly in adults, and there is no significant difference in the incidence between men and women. The treatment of human glioma mainly consists of surgical resection of the tumor, combined with postoperative radiotherapy and chemotherapy. However, patients with glial (high-grade) tumors classified as WHO grades III and IV have an average survival ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/366A61K36/15A61P35/00A61K125/00A61K129/00
CPCA61K31/366A61K36/15A61P35/00
Inventor 葛鹏飞王选重罗毅男
Owner 葛鹏飞
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com