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Nano prodigiosin preparation and application thereof in prevention and treatment of viral diseases of economic crops

A technology for transforming prodigiosin and prodigiosin, which is applied in the application field of microbial secondary metabolites, can solve the problems of short efficacy, inability to effectively control plant viruses, etc., and achieve the effects of improving stability and promoting stability.

Pending Publication Date: 2022-04-05
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a natural secondary metabolite of microorganisms, prodigiosin is easily degraded in the natural environment and has a short efficacy. Therefore, methods such as foliar spraying and root irrigation with solutions cannot effectively control plant viruses.

Method used

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  • Nano prodigiosin preparation and application thereof in prevention and treatment of viral diseases of economic crops
  • Nano prodigiosin preparation and application thereof in prevention and treatment of viral diseases of economic crops
  • Nano prodigiosin preparation and application thereof in prevention and treatment of viral diseases of economic crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A preparation method for a nanoprodigiosin preparation for preventing and treating economic crop virus diseases, comprising the following steps:

[0047] S0-1: Add an equal amount of ssDNA to TM buffer, with a total volume of 100 μL, and gently vortex to mix;

[0048] S0-2: Heat at 95°C for 10 minutes, rapidly cool to 4°C, and keep in a thermal cycler for 20 minutes to maintain the tetrahedral structure;

[0049] S0-3: Incubate the prodigiosin solution and the tFNAs solution at room temperature for 24 hours;

[0050] S0-4: Centrifuge at 10,000×rpm for 10 minutes in a 15kDa ultrafiltration tube to remove unloaded drug and ssDNA;

[0051] Finally, the prepared nanoprodigiosin preparations were stored away from light, and some of them were sent to detect particle size, Zeta potential, PDI and other data. Test results such as Figure 2~3 shown. The detection result shows that the average particle diameter of the preparation is between 57nm and 72nm. Zeta potential -14....

Embodiment 2

[0053] As described in Example 1, the application of a nanoprodigiosin preparation for preventing and treating various economic crop virus diseases in virus treatment, the specific application method is as follows:

[0054] S1-1: Planting and transplanting of laboratory tobacco, tomato and pepper:

[0055] Take 15 disposable plastic cups, and burn a glass rod with an alcohol lamp to poke holes in the bottom of the plastic cups to facilitate the soil to absorb water. Nutrient soil and vermiculite are mixed 1:1 to ensure sufficient nutrition and strong water absorption capacity. Tobacco is first planted in flower pots, and then transplanted into plastic cups after the plant height is suitable. During the transplanting process, the integrity of the tobacco roots and sufficient water supply were ensured, and the constant light was 18 hours a day in the constant temperature room. Wait 2 to 3 weeks.

[0056] S1-2: Inoculation of virus:

[0057] 200 mg of poison source leaves wer...

Embodiment 3

[0086] Example 3 of the present invention provides the effect of the nanoprodigiosin preparation on the host factor Hsp70 protein and its ubiquitination modification.

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Abstract

The invention provides a nanocrystallization prodigiosin preparation based on DNA self-assembly, a preparation method of the nanocrystallization prodigiosin preparation and application of the nanocrystallization prodigiosin preparation in prevention and treatment of crop viruses, belongs to the technical field of application of microbial secondary metabolites, and can solve the problem that the nanocrystallization prodigiosin preparation cannot prevent and treat main economic crop virus diseases (PVY, TMV and CMV). The preparation is prepared from prodigiosin and a DNA tetrahedral nano material, the prodigiosin can significantly improve the ubiquitination level of plant host heat shock protein (HSP70), promote ubiquitination degradation of the host HSP70 protein, degrade or inhibit expression of the HSP70 protein, activate natural immunocompetence of host plants, and achieve the inhibition effect on common RNA viruses. The nanocrystallization prodigiosin preparation provided by the invention is used for preventing and treating virus diseases of main economic crops, the stability of prodigiosin is stronger, the effect is more durable, a good virus disease prevention and treatment effect can be achieved, and the nanocrystallization prodigiosin preparation has a good application prospect in the field of economic crop multi-virus prevention and treatment.

Description

technical field [0001] The invention belongs to the technical field of application of secondary metabolites of microorganisms, and specifically relates to a new preparation method of prodigiosin in anti-economic crop virus disease drugs and a new application in the prevention and treatment of economic crop virus diseases. Background technique [0002] Potato virus Y (PVY) has a wide range of hosts and can infect a variety of plants, especially Solanaceae, followed by Chenopodiaceae and Leguminous plants. Leaf vein necrosis causes significant economic losses, but the virus still lacks effective control measures. [0003] Tobacco mosaic virus disease caused by tobacco mosaic virus (Tobacco mosaic virus, TMV) is one of the most serious diseases of tobacco. TMV has strong stress resistance, simple transmission route, and wide host range. Studies have reported that TMV can infect more than 350 economic crops such as potatoes, tomatoes, and tobacco. The diseased leaves are still...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/36A01N43/90A01P1/00A01P21/00
Inventor 杨金广葛明焦裕冰李莹张万红王凤龙申莉莉宋丽云
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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