Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel application of prodigiosin in resisting potato Y viruses

A prodigiosin, potato technology, applied in application, disinfectant, chemicals for biological control, etc., can solve problems such as unclear anti-plant virus mechanism

Active Publication Date: 2020-11-27
TOBACCO RES INST CHIN AGRI SCI ACAD +2
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing literature has not reported that prodigiosin can resist PVY virus, and the mechanism of anti-plant virus is unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel application of prodigiosin in resisting potato Y viruses
  • Novel application of prodigiosin in resisting potato Y viruses
  • Novel application of prodigiosin in resisting potato Y viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Analysis of prodigiosin-induced tobacco transcriptomics and proteomics

[0036] The plant material used in this experiment is common tobacco NC89 (Nicotiana.tabacum cv.NC89), which was propagated by our laboratory and cultivated in the greenhouse. Experimental plant cultivation conditions: light for 16 hours, temperature 25±1°C, light intensity 2000 lux, relative humidity 60%; dark time for 8 hours, temperature 25±1°C, relative humidity 60%. The nutrient substrate used is produced by the World Nutrient Soil Processing Factory in Shouguang City, Shandong Province, and no additional fertilization is required during plant growth. The sources of TMV and PVY were preserved by the Plant Protection Research Center of the Tobacco Research Institute of the Chinese Academy of Agricultural Sciences, and they were propagated and preserved on the common tobacco NC89 cultivated in the greenhouse. Prodigiosin S3 is isolated and purified from the fermentation broth of Serra...

Embodiment 2

[0039] Example 2: Prodigiosin-induced tobacco proteomics and ubiquitinomics analysis: In order to further understand the mechanism of action of prodigiosin-induced plants to acquire systemic resistance, Nicotiana benthamiana treated with prodigiosin was used as For the target material, the optimal reaction time point of prodigiosin to prevent and treat PVY was selected, and through non-standard quantitative technology, high performance liquid chromatography fractionation technology, enrichment technology of ubiquitinated peptides and quantitative proteomics technology based on mass spectrometry, etc. A series of cutting-edge technologies are organically combined to study the quantitative proteome and ubiquitination non-standard quantitative omics in tobacco leaves.

[0040] protein extraction. Take the leaf sample out from -80°C, put it into a liquid nitrogen pre-cooled mortar, add liquid nitrogen and grind it fully to powder; add 4 times the volume of powder lysis buffer (8M ...

Embodiment 3

[0047] Example 3: Effects of prodigiosin treatment on ubiquitination modification and host factor Hsp70 protein: In order to further confirm the changes in the expression level of ubiquitination and Hsp70 protein after prodigiosin treatment of plants, we used Western blot technology from At the protein level, the changes of Hsp70 protein level and overall ubiquitination level were detected after prodigiosin S3 treatment in Nicotiana benthamiana.

[0048] The result is as Figure 8 As shown, the overall ubiquitination level of Nicotiana benthamiana increased after prodigiosin S3 treatment, and the Ub ubiquitinated protein in the prodigiosin S3 treatment group; at the same time, the Hsp70 protein level in the host decreased. This is consistent with the results of the omics analysis, indicating that the omics data are credible, and the prodigiosin S3-treated plants inhibit the expression of Hsp70 protein in the host. Treatment of tobacco with prodigiosin can significantly increa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of application of microbial secondary metabolites, and particularly relates to novel application of prodigiosin in resisting potato Y viruses. The prodigiosin is applied to preparation of medicines for preventing and controlling potato Y viruses. The medicines for preventing and controlling the potato Y virus are liquid or solid preparations containingprodigiosin. It is proved that a host factor Hsp70 is recruited to promote replication and infection of PVY so as to achieve virus infection and propagation in the host infection process of PVY, so the Hsp70 plays an important role in infection and replication of plant viruses. Based on the above research result, it is further determined that prodigiosin treatment can significantly improve the ubiquitination level of a plant host, promote ubiquitination of the HSP70 protein of the host, degrade or inhibit expression of the Hsp70 protein, activate the natural immune activity of the host plant,induce the plant host to generate systematic resistance and realize antiviral characteristics.

Description

technical field [0001] The invention belongs to the technical field of application of microbial secondary metabolites, and in particular relates to a new application of prodigiosin in resisting potato virus Y (PVY). Background technique [0002] As the largest plant group of plant viruses, Potato virus Y (PVY) is a virus that seriously damages Solanaceae crops. [0003] Potato virus Y (PVY) has a wide range of hosts and is widely distributed all over the world, with an increasing trend year by year. The yield and quality of plants infected by PVY are reduced, resulting in huge economic losses. [0004] PVY is a representative species of the genus Potyvirus in the family Potyviridae. As the largest plant virus genus, Potyvirus has about 200 definite and tentative species, and more than 30% of known plant viruses belong to Potyvirus, including PVY, Turnip mosaic virus (Turnip mosaic virus, Tu MV). ), soybean mosaic virus (Soybean mosaic virus, SMV), plum pox virus (Plum pox...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N43/36A01P1/00
CPCA01N43/36A01P21/00
Inventor 杨金广王凤龙徐翔李莹宋丽云申莉莉雷强冯长春李斌王勇刘东阳江连强
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com