The New Application of Prodigiosin in Anti-Potato-y Virus

A prodigiosin, potato technology, applied in application, disinfectant, chemicals for biological control, etc., can solve problems such as unclear anti-plant virus mechanism

Active Publication Date: 2021-06-25
TOBACCO RES INST CHIN AGRI SCI ACAD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing literature has not reported that prodigiosin can resist PVY virus, and the mechanism of anti-plant virus is unclear

Method used

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  • The New Application of Prodigiosin in Anti-Potato-y Virus
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  • The New Application of Prodigiosin in Anti-Potato-y Virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Analysis of prodigiosin-induced tobacco transcriptomics and proteomics

[0036] The plant material used in this experiment is common tobacco NC89 (Nicotiana.tabacum cv.NC89), which was propagated by our laboratory and cultivated in the greenhouse. Experimental plant cultivation conditions: light for 16 hours, temperature 25±1°C, light intensity 2000 lux, relative humidity 60%; dark time for 8 hours, temperature 25±1°C, relative humidity 60%. The nutrient substrate used is produced by the World Nutrient Soil Processing Factory in Shouguang City, Shandong Province, and no additional fertilization is required during plant growth. The sources of TMV and PVY were preserved by the Plant Protection Research Center of the Tobacco Research Institute of the Chinese Academy of Agricultural Sciences, and they were propagated and preserved on the common tobacco NC89 cultivated in the greenhouse. Prodigiosin S3 is isolated and purified from the fermentation broth of Serra...

Embodiment 2

[0039] Example 2: Prodigiosin-induced tobacco proteomics and ubiquitinomics analysis: In order to further understand the mechanism of action of prodigiosin-induced plants to acquire systemic resistance, Nicotiana benthamiana treated with prodigiosin was used as For the target material, the optimal reaction time point of prodigiosin to prevent and treat PVY was selected, and through non-standard quantitative technology, high performance liquid chromatography fractionation technology, enrichment technology of ubiquitinated peptides and quantitative proteomics technology based on mass spectrometry, etc. A series of cutting-edge technologies are organically combined to study the quantitative proteome and ubiquitination non-standard quantitative omics in tobacco leaves.

[0040] protein extraction. Take the leaf sample out from -80°C, put it into a liquid nitrogen pre-cooled mortar, add liquid nitrogen and grind it fully to powder; add 4 times the volume of powder lysis buffer (8M ...

Embodiment 3

[0047] Example 3: Effects of prodigiosin treatment on ubiquitination modification and host factor Hsp70 protein: In order to further confirm the changes in the expression level of ubiquitination and Hsp70 protein after prodigiosin treatment of plants, we used Western blot technology from At the protein level, the changes of Hsp70 protein level and overall ubiquitination level were detected after prodigiosin S3 treatment in Nicotiana benthamiana.

[0048] The result is as Figure 8 As shown, the overall ubiquitination level of Nicotiana benthamiana increased after prodigiosin S3 treatment, and the Ub ubiquitinated protein in the prodigiosin S3 treatment group; at the same time, the Hsp70 protein level in the host decreased. This is consistent with the results of the omics analysis, indicating that the omics data are credible, and the prodigiosin S3-treated plants inhibit the expression of Hsp70 protein in the host. Treatment of tobacco with prodigiosin can significantly increa...

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Abstract

The invention belongs to the technical field of application of microbial secondary metabolites, and in particular relates to a new application of prodigiosin in anti-potato virus Y. The application of the prodigiosin in the preparation of drugs for the prevention and control of potato virus Y. Drugs for the prevention and control of potato virus Y are liquid or solid preparations containing prodigiosin. It is clear that PVY promotes its own replication and infection by recruiting the host factor Hsp70 in the process of infecting the host, and realizes the infection and transmission of the virus. Hsp70 plays an important role in the infection and replication of plant viruses. Based on the above research results, it is further clarified that prodigiosin treatment can significantly increase the ubiquitination level of the plant host, promote the ubiquitination of the host HSP70 protein, degrade or inhibit the expression of the Hsp70 protein, activate the natural immune activity of the host plant, and induce The host develops systemic resistance and achieves antiviral characteristics.

Description

technical field [0001] The invention belongs to the technical field of application of microbial secondary metabolites, and in particular relates to a new application of prodigiosin in resisting potato virus Y (PVY). Background technique [0002] As the largest plant group of plant viruses, Potato virus Y (PVY) is a virus that seriously damages Solanaceae crops. [0003] Potato virus Y (PVY) has a wide range of hosts and is widely distributed all over the world, with an increasing trend year by year. The yield and quality of plants infected by PVY are reduced, resulting in huge economic losses. [0004] PVY is a representative species of the genus Potyvirus in the family Potyviridae. As the largest plant virus genus, Potyvirus has about 200 definite and tentative species, and more than 30% of known plant viruses belong to Potyvirus, including PVY, Turnip mosaic virus (Turnip mosaic virus, Tu MV). ), soybean mosaic virus (Soybean mosaic virus, SMV), plum pox virus (Plum pox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/36A01P1/00
CPCA01N43/36A01P21/00
Inventor 杨金广王凤龙徐翔李莹宋丽云申莉莉雷强冯长春李斌王勇刘东阳江连强
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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