Application of potato-like phospholipase 7

A phospholipid and potato-like technology, applied in the field of biomedicine, can solve problems such as unknown physiological functions in the body

Inactive Publication Date: 2019-10-29
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Potato-like phospholipase domain containing protein 7 (Patatin-like phospholipase domain containing protein 7, PNPLA7, NM _146251.4) is a PNPLA family that is highly expressed in adipose tissue, liver and muscle tissue, has lysophospholipase hydrolysis activity in vitro, but has unknown physiological functions in vivo protein

Method used

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  • Application of potato-like phospholipase 7
  • Application of potato-like phospholipase 7
  • Application of potato-like phospholipase 7

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Such as figure 1 As shown in -A, 5 normal human liver samples and 12 fatty liver disease patient samples were collected, RNA was extracted, and the expression level of PNPLA7 gene was detected. By further analyzing the relationship between serum TAG and TC levels and PNPLA7 expression in 17 samples, it was found that PNPLA7 was positively correlated with TAG and TC levels in human blood.

Embodiment 2

[0027] After the knockdown of PNPLA7 in the liver, the TAG content in the liver of the mice was significantly increased, and the serum TAG was significantly reduced

[0028] Knockdown PNPLA7 adenovirus (knockdown sequence shown in SEQ ID NO.1) was injected into the mouse tail vein to knock down PNPLA7 in the liver ( figure 2 -A and B). The liver and blood of the mice were collected 7 days after the virus injection, and the liver lipids were extracted by the Folch method, and the TAG content of the mouse liver and the TAG content of the mouse serum were detected by using the TAG detection kit (WAKO). The content increased significantly, and the serum TAG content decreased significantly.

Embodiment 3

[0030] After knockdown of PNPLA7 in the liver, the rate of VLDL secretion is reduced

[0031] Hepatic PNPLA7 knockdown was performed by injecting knockdown PNPLA7 adenovirus through mouse hypertensive tail vein.

[0032] After 5 days of tail vein injection of adenovirus, the mice were fasted for 8 h, and 500 mg / kg body weight of TritonWR-1339 was injected into the tail vein to block the clearance and degradation of VLDL in the blood of mice. Take the blood at time points 0, 1, 2, and 3 h, let it stand at room temperature for 1 h, centrifuge at 4000 rpm, 4 °C for 30 min, transfer the supernatant, centrifuge again at 13000 rpm, 4 °C, 10 min, transfer the supernatant to a new Centrifuge the tube, and detect the TAG content in the serum to represent the amount of VLDL in the serum. Such as image 3 As shown in -A, after knocking down PNPLA7 in the liver, the secretion of VLDL in the liver of the mice was significantly reduced.

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Abstract

The invention relates to an application of potato-like phospholipase 7. As a molecular target for treating non-alcoholic fatty liver disease and the hyperlipidemia accompanying the non-alcoholic fattyliver disease, the potato-like phospholipase 7 can be applied to the preparation of a medicament for treating the non-alcoholic fatty liver disease and the hyperlipidemia accompanying the non-alcoholic fatty liver disease. The invention also discloses an application of adenovirus or lentivirus for reducing the gene expression of the potato-like phospholipase 7 in the preparation of a medicament for treating hyperlipidemia. The knock-down sequence of the adenovirus is as shown in SEQ ID NO.1, and the knock-down sequence of the lentivirus is as shown in SEQ ID NO.2. Finally, the invention alsocomprises an application of the potato-like phospholipase 7 in preparation of a kit for screening, treating and detecting non-alcoholic fatty liver and hyperlipemia.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of potato-like phospholipase 7 protein in nonalcoholic fatty liver disease and hyperlipidemia. Background technique [0002] With the change of dietary structure, obesity and nonalcoholic fatty liver disease (Nonalcoholic Fatty Liver Disease, NAFLD) caused by overnutrition have become a global epidemic disease. Increased incidence of obesity and NAFLD will directly lead to increased incidence of chronic liver disease. [0003] NAFLD is a pathological syndrome of excessive liver lipid accumulation caused by alcohol and other definite liver damage factors such as alcohol and drugs. It is a series of pathological processes that occur after liver lipid accumulation. The incidence of NAFLD in my country is increasing year by year. Fatty liver disease will further cause liver fibrosis, liver cirrhosis and liver cancer, which seriously threatens people's health....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/46A61K48/00A61P1/16A61P3/06G01N33/68G01N33/573
CPCA61K38/465A61K48/0025A61K48/005A61P1/16A61P3/06G01N33/573G01N33/68G01N2333/918G01N2800/085
Inventor 李仲王秀云王倩郭敏张许
Owner NANJING MEDICAL UNIV
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