Application of Shuanghuanglian preparation in virus infection resistance

A coronavirus, a technology for use, applied in the field of medicine, can solve the problems of limited effect of coronavirus, lack of effective treatment of acute viral infectious diseases, etc.

Pending Publication Date: 2021-08-03
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, current drugs have limited effectiveness against coronaviruses that cause acute infectious diseases, such as SARS virus, MERS virus, and 2019-

Method used

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  • Application of Shuanghuanglian preparation in virus infection resistance
  • Application of Shuanghuanglian preparation in virus infection resistance
  • Application of Shuanghuanglian preparation in virus infection resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1. Chlorogenic acid is to the inhibitory effect of SARS virus activity

[0078] In this example, through anti-SARS virus (virus strain: BJ-01) activity screening (virus-cell level model), it is verified whether chlorogenic acid has an inhibitory effect on SARS virus.

[0079] Test principle: Vero-E6 cells are used as virus host cells (susceptible cells) to test the effect of samples against virus-infected cells. The detection indicators are cell degeneration (CPE) and the protection rate of infected cells.

[0080] testing method:

[0081] Seed Vero-E6 cells in a 96-well culture plate at 37°C, 5% CO 2 Cultivate, add samples and SARS virus of different dilution concentrations respectively, establish virus control, cell control and sample control. Observe the results under the microscope every day, record the CPE, and measure the OD value with neutral red staining, and calculate and evaluate the anti-SARS virus activity of the sample with reference to the con...

Embodiment 2

[0085] Example 2. The inhibitory effect of Shuanghuanglian preparation (oral solution) on avian influenza virus.

[0086] In this example, the anti-influenza virus activity screening (neuraminidase activity inhibition test) was used to test the inhibitory effect of Shuanghuanglian preparation (oral solution) on avian influenza virus.

[0087] Virus strains: Avian influenza virus strain H5N1 (2004), Tamiflu-resistant influenza virus strain H1N1

[0088] Shuanghuanglian preparation (oral solution): commercially available.

[0089] Test principle:

[0090] Neuraminidase (NA) is an important glycoprotein on the envelope of influenza virus, which plays an important role in the replication and transmission of influenza virus. Inhibitors of influenza virus neuraminidase (NA) are the targets of Tamiflu, a commonly used clinical anti-influenza virus drug. Using influenza virus neuraminidase as an active drug screening and evaluation target can efficiently and rapidly screen and eval...

Embodiment 3

[0100] Inhibitory effect of Shuanghuanglian preparation (oral solution) on MERS-CoV virus activity

[0101] In this example, through the anti-MERS-CoV pseudovirus invasion activity screening (virus-cell level model), it was verified whether the Shuanghuanglian preparation (oral solution) had an inhibitory effect on the MERS-CoV virus.

[0102] Test principle: Huh7 cells are used as virus host cells (susceptible cells), and the test sample blocks the activity of MERS-CoV membrane protein modified pseudovirus-infected cells, which can reflect the antiviral activity of the sample to interfere with the key target of MERS-CoV infection . The detection index is the activity level of the reporter gene on the pseudovirus genome.

[0103] testing method:

[0104] Huh7 cells were inoculated in 96-well culture plates one day in advance, and the activity plate and cytotoxicity plate were respectively set up, and cultured at 37°C and 5% CO2. The activity assay plate and the cytotoxicity...

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Abstract

The invention relates to application of a Shuanghuanglian preparation in virus infection resistance. Specifically, the invention provides an application of a chlorogenic acid compound or a pharmaceutically acceptable salt or an extract of the chlorogenic acid compound. Experiments show that the Shuanghuanglian preparation (oral liquid) and the active ingredient chlorogenic acid thereof have obvious antiviral effects on severe acute respiratory syndrome coronavirus (SARS-CoV), influenza virus (influenza virus), Middle East Respiratory Syndrome (MERS) and 2019-nCoV (new coronavirus), and prove that the Chinese patent medicine Shuanghuanglian preparation (oral liquid) can be used for treating virus infectious diseases.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of Shuanghuanglian preparation in antiviral infection. Background technique [0002] Among the acute infectious diseases, most of them are viral infectious diseases, and the incidence rate of viral infectious diseases is high, and the mortality rate is also high. [0003] In 2003, my country and many countries in the world were hit by a large-scale atypical pneumonia (Atypical pneumonias), that is, severe acute respiratory syndrome (Severe Acute Respiratory Syndrome, SARS) caused by atypical pneumonia. The pathogen of SARS is a single-stranded RNA virus named SARS coronavirus. [0004] In 2012, another coronavirus attacked countries such as Saudi Arabia in the Middle East. The virus was named Middle East Respiratory Syndrome Coronavirus (Middle East Respiratory Syndrome Coronavirus, MERS-CoV) or MERS virus. MERS (Middle East Respiratory Syndrome). [0005] At ...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61K36/634A61P31/14A01N37/38A01N65/22A01N65/08A01P1/00
CPCA61K31/216A61K36/355A61K36/634A61K36/539A61P31/14A01N65/00A01N37/38A01N65/22A01N65/08
Inventor 左建平唐炜童贤崑杨莉何佩岚周宇赵维民肖庚富张磊砢蒋华良
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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