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Fusion protein having antibiotic function and uses thereof

A technology of fusion protein and function, applied in the field of fusion protein and its application, can solve the problems of restricting wide application, environmental pollution, frequent occurrence of aquaculture diseases, etc.

Inactive Publication Date: 2008-05-21
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although antimicrobial peptides have remarkable antibacterial efficacy and broad application prospects, the content of natural antimicrobial peptides is very low and cannot be obtained in large quantities from organisms; and chemically synthesized peptides are expensive, which limits their wide application
At present, due to serious environmental pollution, aquaculture diseases frequently occur, often causing relatively large economic losses

Method used

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  • Fusion protein having antibiotic function and uses thereof
  • Fusion protein having antibiotic function and uses thereof
  • Fusion protein having antibiotic function and uses thereof

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Embodiment Construction

[0034] Below with embodiment and in conjunction with accompanying drawing, content of the present invention will be further described:

[0035] 1. Preparation of fish liver cDNA: 24 hours after giant flounder was injected with Vibrio anguillarum, the liver was taken out, quickly placed in liquid nitrogen, and the hard tissue pieces were frozen at -80°C for preservation. Refer to TRIZOLReagent operation instructions, take about 100mg of frozen preserved tissue, add it to 1ml TRIZOL reagent, homogenize at room temperature for 5 minutes, add 200μl chloroform to shake vigorously for 15 seconds, and incubate at room temperature for 3 minutes. Centrifuge at 12,600 rpm for 15 min at 4°C. Add 250μl of high-concentration salt solution composed of 0.8M sodium acetate and 1.2M sodium chloride to obtain the upper layer of the interface, mix well, then add 250μl isopropanol and mix well, settle at room temperature for 10min, and then rotate at 12,600rpm at 4℃ Centrifuge for 10 min. A...

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Abstract

The invention discloses a fusion protein with antibacterial function and its application. The fusion protein gene sequence consists of a cloned hepcidinl precursor antimicrobial peptide gene and an artificially synthesized magainin antimicrobial peptide gene, which are composed of a novel fusion protein cDNA and integrated into the yeast genome by genetic engineering means, and induced The fusion protein cDNA is expressed in yeast, and then a large amount of fusion protein is obtained through yeast fermentation. The fusion protein of the present invention has obvious antibacterial activity after testing, and can be used for the prevention and treatment of bacterial diseases of aquatic organisms and farmed animals, or the purified fusion protein can be directly used as medicine to replace antibiotics for diseases caused by bacteria or even viruses Treatment.

Description

technical field [0001] The present invention relates to a fusion protein and its application, in particular to an animal antimicrobial peptide gene carrier established by genetic engineering technology and an antimicrobial peptide fusion protein obtained through a yeast expression system, and its application in the preparation of antibacterial drugs or disease prevention and control functions. Application in aquatic organisms or farmed livestock and poultry feed products. Background technique [0002] Antimicrobial peptides are the immune substances produced by the organism itself to resist the infection of external microorganisms, viruses and other pathogens, and are used for non-specific defense of the body. It widely exists in animals and plants, and has the characteristics of high bactericidal activity, rapid action, and no drug resistance. It is an effective means of multicellular biological immune defense and plays an important role in the survival and evolution of org...

Claims

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Application Information

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IPC IPC(8): C07K19/00C07K14/46A61K38/16C12N15/62A61P31/04
Inventor 丛柏林刘晨临林学政刘胜浩黄晓航
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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