Application of corn transcription factor ZmMYB42 gene to drought-resistant breeding of plants

A transcription factor, corn technology, applied in the field of bioengineering breeding of crops, can solve the problems that ZmMYB42 has not been seen, the expression level and the change of expression space-time characteristics are difficult to predict, and the plant morphological characteristics, stress resistance and yield have not been seen.

Active Publication Date: 2021-02-05
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the reduction of plant cell wall lignin content may reduce the resistance of plants to insect pests and pathogens and promote the growth of plants under suitable conditions, plant stress resistance and growth and development are regulated by complex multiple signals and metabolic pathways. Changes in the expression level and spatiotemporal characteristics of a transcription factor often produce unpredictable effects
In addition, due to the large number of members of the maize MYB family and the diverse and complex protein structures and expression profiles, the use of homologous gene research data to deduce the function of a specific MYB gene often cannot withstand experimental verification, and reliable conclusions can only be obtained through genetic manipulation.
So far, there have been no reports on whether ZmMYB42 has a function in the stress resistance of maize or sorghum, nor the effects of overexpression of ZmMYB42 on plant morphological characteristics, stress resistance and yield in maize or sorghum crops. Whether the transgene is driven by a stress-inducible or constitutive promoter

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Transformation of the ZmMYB42 gene to create drought-tolerant inbred lines in maize

[0033] 1) Construction of ZmMYB42 gene transformation vector and Agrobacterium-mediated transformation

[0034] The ZmMYB42 gene coding frame was fused with the stress-inducible promoter Prd29B or the constitutive promoter Pubiquitin1, and the fusion gene was inserted into the plant expression vector pCambia1300-PCaMV35S::bar by conventional gene recombination technology to produce

[0035] Transformation plasmid pCambia1300-Prd29B::ZmMYB42-PCaMV35S::bar

[0036] or pCambia1300-Pubiquitin1::ZmMYB42-PCaMV35S::bar.

[0037] Then the Escherichia coli competent cells were prepared by the calcium chloride method, and the recombined transformation plasmids were transferred, positive clones were screened out, and the plasmids were extracted for T-DNA region sequencing identification. Then the clones with correct sequencing were cultivated to extract a large number of transformati...

Embodiment 2

[0050] Example 2: Transformation of the ZmMYB42 gene to create new drought-tolerant sorghum materials

[0051] 1) Establishment of receptor system

[0052] The sorghum varieties or sterile lines, maintainer lines, and restorer lines used in my country's agricultural production are used as materials. The seeds are soaked in 70% ethanol for 6 minutes, then soaked in 0.1% mercuric chloride for 8-10 minutes, and then washed 3-5 times with sterile water. Shake the seeds constantly during sterilization to ensure complete surface sterilization. After sterilization, the seeds are placed in a sterile Erlenmeyer flask to germinate under dark conditions (25-28° C.), and a small amount of sterile water is put into the bottle. After 2-3 days, the seeds germinated (lubai), and they were broadcast on the modified MS medium to continue to germinate under dark conditions. When the germ elongates to about 3 cm, the coleoptile and young leaves are peeled off to expose the growth cone at the t...

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PUM

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Abstract

The invention discloses an application of a corn transcription factor ZmMYB42 gene to drought-resistant breeding of plants. A gene ZmMYB42 is cloned from corn, the gene or a RNAi structure thereof isrecombined into a plant expression vector in a sense form, a fusion gene or a ZmMYB42RNAi structure is introduced into crops by using a transgenic technology, drought resistance determination is performed on transgenic plants, transgenic plants with obviously improved or reduced drought resistance and progenies thereof are screened out, and new plant germplasm with application value is created. The cDNA sequence of the corn transcription factor ZmMYB42 is as shown in SEQ ID No. 1, and the coded amino acid sequence of the corn transcription factor ZmMYB42 is as shown in SEQ ID No. 2. The plantsrefer to cultivated cereal crops. The application disclosed by the invention is of great significance for increasing the crop yield under drought stress.

Description

technical field [0001] The invention belongs to the field of bioengineering breeding of crops, and in particular relates to the application of a corn transcription factor ZmMYB42 gene in plant drought-resistant breeding. Background technique [0002] The MYB family of transcription factors refers to a class of transcription factors that contain a highly conserved DNA-binding domain, namely the MYB domain. The MYB domain is a peptide segment of about 51-52 amino acids, including a series of highly conserved amino acid residues and spacer sequences. The earliest discovered MYB transcription factors are v-MYB of avian myeloblastoma virus, A-MYB involved in sperm formation in vertebrates, B-MYB that plays an important role in delaying aging and activating proteins, and plays an important role in reducing cancer C-MYB as a regulator of cell viability and hematopoietic neurogenesis. Plant transcription factor MYB is one of the largest transcription factor families in plants. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84A01H5/00A01H6/46
CPCC12N15/8273C12N15/8218C07K14/415
Inventor 张举仁李朝霞李鹏
Owner SHANDONG UNIV
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