Rice leaf color control gene heme oxygenase2 (HO2) and application thereof

A gene and leaf color technology, applied in the field of transgenic complementary function experiment to identify the gene, rice HO2 gene, can solve the problems that the function has not been fully clarified, and achieve the effect of improving photosynthesis efficiency and yield

Inactive Publication Date: 2013-05-22
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]At present, only a few genes of rice virescent mutants have been identified, and their functions have not been fully elucidated

Method used

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  • Rice leaf color control gene heme oxygenase2 (HO2) and application thereof
  • Rice leaf color control gene heme oxygenase2 (HO2) and application thereof
  • Rice leaf color control gene heme oxygenase2 (HO2) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Mapping of HO2 gene

[0022] 1. Isolation and genetic analysis of ho2 of rice leaf color mutants:

[0023] Through the screening of the rice (Nipponbare) mutant library induced by radiation mutagenesis, the inventors isolated and obtained a rice yellow leaf mutant ho2 such as figure 1 shown.

[0024] 1. Materials and methods

[0025] Rice (Oryza sativa ssp. japonica Nipponbare) mutant ho2 (heme oxygenase2), from the rice (Nipponbare) mutant library induced by radiation.

[0026] Training process and conditions:

[0027] The rice seeds were washed with distilled water, placed in a petri dish, soaked for 2 days in the dark at 28°C until they turned white, then transferred to a nylon gauze, and grown in the light for 7 days. The seedlings grown for 7 days were carefully removed from the gauze, and transplanted to sponge balls on PVC plates to continue culturing in rice nutrient solution. The specific conditions are as follows:

[0028] In the light and...

Embodiment 2

[0054] Example 2: Functional identification of HO2 gene

[0055] Plant Transformation:

[0056] A complementary vector containing 993 bp of the full-length coding region of HO2 (Seq ID No.1) with 35S as the promoter and NOS as the terminator was constructed, namely 35S::HO2(1220bp)::NOS ( Figure 4 ), transformed into Agrobacterium EHA105, transformed rice ho2 mutant. The transformation method was Agrobacterium-mediated method. The experimental results found that the complementary vector can completely restore the mutant phenotype of ho2 ( image 3 ). Among them, such as Figure 4 shown PC 35SHO2, the vector can express the polypeptide encoded by the above nucleotide sequence or its homologous analogue.

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Abstract

The invention discloses a rice leaf color control gene heme oxygenase2 (HO2) and an application thereof. A gene which is cloned from a rice leaf control mutant ho2 and is proved to have the effect of controlling the color of a rice leaf is named after HO2. A transgene functional complementation assay proves that the HO2 is the gene which controls the color of the rice leaf. Comparative analysis on amino acid sequences shows that HO2 protein belongs to a hemeoxygenase protein family. Phenotype observation and biophysical analysis prove that the cloned gene has the effects of regulating and controlling the development of chloroplasts and then controlling the color of the leaf. The invention also discloses an application of the rice leaf color control gene HO2 in fine seed breeding and cross breeding by taking a green yellow leaf character as a marker character by using genetic engineering. The rice leaf color control gene HO2 is obtained by using a map-based cloning technology, and the effects of the gene is tested through the transgene functional complementation test. Therefore, the rice leaf color control gene HO2 has the effect of ensuring rice to keep an appropriate leaf color, so that the photosynthetic efficiency of the rice is improved, and finally the rice yield can be improved.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, the present invention relates to a rice HO2 (heme oxygenase2) gene cloned by map-based cloning technology, and the identification of the gene through a transgene complementary function experiment; it also relates to the use of the gene to regulate the development of rice chloroplasts, thereby regulating the chlorophyll content of rice leaves , Improve photosynthetic efficiency and increase crop yield. Background technique [0002] The chloroplast genome of higher plants generally encodes about 100 proteins, while the chloroplast contains at least more than 2000 proteins. Thus, the vast majority of chloroplast proteins are encoded by nuclear genes, translated in the cytoplasm and transported into the chloroplast (Abdallah et al., 2000). The transcription of plastid genes is accomplished by two types of RNA polymerases, plastid-encoded RNA polymerase (PEP) and nuclear gene-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N15/53C12N15/63C12N15/82C12N1/21C12N5/10A01H5/00
Inventor 刘洪家李青竹卢思聪刘合芹翟国伟邵健丰陶跃之
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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