A kind of production method of lactic acid bacteria nitrite reductase

A lactic acid bacteria nitrite, production method technology, applied in microorganism-based methods, biochemical equipment and methods, enzymes and other directions, can solve the problems of high production cost, many components, complex preparation process and the like of lactic acid bacteria nitrite reductase , to achieve the effect of improving food safety and quality, reducing production cost and simple production process

Inactive Publication Date: 2011-12-21
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The formula has many components, the cost is relatively high, and the preparation is relatively complicated
As a resul

Method used

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  • A kind of production method of lactic acid bacteria nitrite reductase
  • A kind of production method of lactic acid bacteria nitrite reductase
  • A kind of production method of lactic acid bacteria nitrite reductase

Examples

Experimental program
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Effect test

Embodiment 1

[0067] (1) Activation of strains

[0068] Inoculate Lactobacillus plantarum into MRS medium at 5% inoculum, and culture at 30°C for 24 hours to obtain the first-generation strains, and then inoculate the first-generation strains with 3% (V / V) inoculum into MRS Culture medium, cultivated at 30°C for 24 hours to obtain the second-generation strain;

[0069] (2) Inoculate the second-generation strain obtained in step (1) into the seed medium with an inoculum amount of 5% (V / V), and cultivate it at a temperature of 33-37°C for 10-15 hours to obtain the production strain ;

[0070] The seed culture medium is composed of cabbage juice, tomato juice and water, and the cabbage juice, tomato juice and water are calculated according to the volume ratio, that is, cabbage juice: tomato juice: water is 0.35: 0.35: 0.30, which also contains 0.2 ~ 0.3g / 100mL KH 2 PO 4 , culture based on sterilization at 121°C for 15 min;

[0071] (3) Inoculate the production strain obtained in step (2)...

Embodiment 2

[0078] (1) Activation of strains

[0079] Inoculate Lactobacillus brevis in MRS medium at 5% inoculum, and culture at 30°C for 24 hours to obtain the first-generation strains, and then inoculate the first-generation strains with 5% (V / V) inoculum in MRS medium , cultivated at 30°C for 24 hours to obtain the second-generation strain;

[0080] (2) Inoculate the second-generation strains obtained in step (1) into the seed medium with an inoculum amount of 5% (V / V), and cultivate them at a temperature of 37°C for 15 hours to obtain production strains;

[0081] The seed medium is composed of cabbage juice, tomato juice and water, which also contains 0.3g / 100mL KH 2 PO 4 ;

[0082] The ratio of cabbage juice, tomato juice and water is 0.50:0.40:0.10;

[0083] Cultivate based on sterilization at 121°C for 15 minutes;

[0084] (3) Inoculate the production strain obtained in step (2) into the fermentation medium with an inoculum amount of 10% (V / V), culture at 35°C for 24 hours, a...

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Abstract

The invention discloses a production method of lactic acid bacteria nitrite reductase, that is, lactic acid bacteria are activated and cultivated with MRS liquid medium for 2 to 3 generations, and the activated bacteria are obtained by using a seed medium composed of cabbage juice, tomato juice, etc. and fermentation culture The lactic acid bacteria fermented liquid is obtained after medium culture, and the pH value of the lactic acid bacteria fermented liquid is adjusted to 6.3-6.5 with sodium hydroxide, and then 0.4-0.6mg/mL sodium nitrite is added, and the lactic acid bacteria obtained after 32-48 hours of cultivation at 37°C are reduced to nitrite The enzyme fermentation broth is centrifuged to collect the bacteria, and then the bacteria cells are further broken, and the supernatant is collected, and then the nitrite reductase is finally obtained through steps such as precipitation, dialysis, and freeze-drying. The production method of the lactic acid bacteria nitrite reductase of the present invention not only has low production cost, but also has the advantages of simple production process, more convenient operation and the like.

Description

technical field [0001] The invention relates to microbial fermentation production technology, in particular to a fermentation production method of lactic acid bacteria nitrite reductase. Background technique [0002] Nitrite is one of the main chemical pathogenic factors of food poisoning in my country in recent years. After nitrite is absorbed into the blood, it can make Fe in normal hemoglobin 2+ Oxidized to Fe 3+ , causing hemoglobin to lose its oxygen-carrying function, resulting in tissue hypoxia, and even hypoxia asphyxia. Nitrite is also a precursor of strong carcinogen nitrosamines, which is a potential carcinogenic hazard. It is closely related to the occurrence of gastric cancer, liver cancer and esophageal cancer. According to the epidemiological survey, the nitrite content in drinking water of residents in areas with high incidence of cancer is higher than that in areas with low incidence. [0003] At present, due to the extensive use of nitrogen fertilizers...

Claims

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Application Information

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IPC IPC(8): C12N9/06C12R1/25C12R1/24C12R1/01
Inventor 龚钢明何婷婷管世敏吕玉涛丁少南
Owner SHANGHAI INST OF TECH
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