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Method for producing nitrite reductase by ferment of lactic acid bacteria

A technology of lactic acid bacteria fermentation and nitrous acid, applied in the direction of oxidoreductase, bacteria, etc.

Inactive Publication Date: 2005-10-26
张庆芳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The new research shows that there is no scientific, convenient, effective and fast method to eliminate nitrite residues in agricultural products, feed, food and the environment at home and abroad

Method used

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  • Method for producing nitrite reductase by ferment of lactic acid bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Culture medium preparation

[0016] ①Strain activation medium: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, diamine citrate 2.0g, Tween 80 1.0g, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·H 2 O0.05g, CaCO 3 20.0g, 15.0g agar, 1.0L distilled water, pH 6.8, sterilized by high pressure steam at 121℃ for 30min.

[0017] ② Liquid seed medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast extract, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, 1.0g Tween 80, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·H 2 O0.05g, CaCO 3 20.0g, 1.0L tap water, pH value 7.0, sterilized by high pressure steam at 121℃ for 30min.

[0018] ③Enzyme production medium: 3.0-7.0g casein peptone, 2.0-8.0g beef extract, 3.0-9.0g yeast extract, 3.0-10.0g corn starch sugar, 0.5-2.0g sodium acetate, 0.6-2.0g diamine citrate 2.0g, Tween80 0.3~1.4g, K 2 HPO 4 0.4~1.5g, CaCO 3 20.0g, 1.0L tap water, pH value 7.0, s...

Embodiment 2

[0028] (1) Culture medium preparation

[0029] ①Strain activation medium: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, diamine citrate 2.0g, Tween 80 1.0g, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·H 2 O0.05g, CaCO 3 20.0g, 15.0g agar, 1.0L distilled water, pH 6.8, sterilized by high pressure steam at 121℃ for 30min.

[0030] ② Liquid seed medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast extract, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, 1.0g Tween 80, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·H 2 O0.05g, CaCO 3 20.0g, 1.0L tap water, pH value 7.0, sterilized by high pressure steam at 121℃ for 30min.

[0031] ③Enzyme production medium: 3.0-7.0g casein peptone, 2.0-8.0g beef extract, 3.0-9.0g yeast extract, 3.0-10.0g corn starch sugar, 0.5-2.0g sodium acetate, 0.6-2.0g diamine citrate 2.0g, Tween80 0.3~1.4g, K 2HPO 4 0.4~1.5g, CaCO 3 20.0g, 1.0L tap water, pH value 7.0, st...

Embodiment 3

[0041] (1) Culture medium preparation

[0042] ①Strain activation medium: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, diamine citrate 2.0g, Tween 80 1.0g, K 2 HPO 4 1.0g, MgS0 4 ·7H 2 O0.2g, MnSO 4 ·H 2 O0.05g, CaCO 3 20.0g, 15.0g agar, 1.0L distilled water, pH 6.8, sterilized by high pressure steam at 121℃ for 30min.

[0043] ② Liquid seed medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast extract, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, 1.0g Tween 80, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·H 2 O0.05g, CaCO 3 20.0g, 1.0L tap water, pH value 7.0, sterilized by high pressure steam at 121℃ for 30min.

[0044] ③Enzyme production medium: 3.0-7.0g casein peptone, 2.0-8.0g beef extract, 3.0-9.0g yeast extract, 3.0-10.0g corn starch sugar, 0.5-2.0g sodium acetate, 0.6-2.0g diamine citrate 2.0g, Tween80 0.3~1.4g, K 2 HPO 4 0.4~1.5g, CaCO 3 20.0g, 1.0L tap water, pH value 7.0, st...

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Abstract

The invention provide a method for producing nitrite reductase by ferment of lactic acid bacteria, activating lactic acid bacteria, enlarge breeding gradually, preparation liquid seeds, according to ferment liquid 3%-8% put it into the liquid zymogenic culture medium, in the temperature of 26-32 Deg. C breeding 36-72h, adding 40-150mg / L nitrite, continue breeding 12-24h.

Description

technical field [0001] The invention relates to the fields of microbiology, enzyme engineering, fermentation engineering, biochemistry, food chemistry, food science, food safety and the like, and in particular relates to a method for producing nitrite reductase by fermentation of lactic acid bacteria. The nitrite reductase produced by the invention is mainly used to eliminate nitrite residues in agricultural products, food, feed and the environment, improve food safety and food quality, and reduce the incidence of food poisoning and cancer caused by nitrite. Background technique [0002] Agricultural production uses a large amount of nitrogen fertilizer, and the ratio of N, P, and K is out of balance, which makes a large amount of nitrate and nitrite enrichment in the environment, resulting in a large amount of nitrite residue in agricultural products, food and feed. Nitrite is a substance harmful to the human body. It not only directly poisons and kills humans, livestock an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02
Inventor 张庆芳迟乃玉
Owner 张庆芳
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