Reagent (kit) for measuring formaldehyde and method for measuring concentration of formaldehyde

Abstract

The invention relates to a reagent (kit) for measuring formaldehyde by using an enzyme-multiplied method, an enzyme colorimetric method and an enzyme coupling method as well as a method for measuring the concentration of the formaldehyde, and composition and ingredients of the reagent, belonging to the technical field of food / environmental test. The reagent (kit) comprises the main ingredients of buffer solution, coenzyme, formaldehyde dehydrogenase, hydrogenlyase and stabilizing agent. The concentration of the formaldehyde is measured by mixing a sample and the reagent according to a certain volume ratio to carry out a series of enzymatic reaction, placing the reactant under an ultraviolet / visible light analyzer and detecting the ascending degree of absorbance at a dominant wavelength of 340nm.

Description

technical field

[0001] The invention relates to a reagent (box) for measuring formaldehyde, and at the same time, the invention also relates to a method for measuring formaldehyde concentration, which belongs to the technical field of food / environmental inspection and determination. Background technique

[0002] The determination methods of formaldehyde mainly include spectrophotometry, chromatography, electrochemical method, chemical titration, etc.:

[0003] The main methods for spectrophotometric determination are acetylacetone method, chromic acid method, MBTH method, fumagenta method, AHMT method and so on. The advantage of spectrophotometry is that it is easy to operate; the disadvantage is that it has low sensitivity, polluted medium, and interference to the measurement.

[0004] Chromatography mainly includes gas chromatography, high performance liquid chromatography, ion chromatography, etc. Chromatography is rarely used directly, and it is generally used in conjun...

Examples

Embodiment 1

[0040] The formaldehyde determination reagent of the present embodiment is a single reagent, comprising:

[0041] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0042] Stabilizer 500mmol / L

[0043] Coenzyme 3mmol / L

[0044] Formaldehyde dehydrogenase 10000U / L

[0045] Formate dehydrogenase 12000U / L

[0046] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0047] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured formaldehyde sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0048] After adding the sample and reagent, let them mix and rea...

Embodiment 2

[0050] The formaldehyde determination reagent of the present embodiment is double reagent, comprises:

[0051] Reagent 1

[0052] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0053] Stabilizer 50mmol / L

[0054] Coenzyme 3mmol / L

[0055] Reagent 2

[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Formaldehyde dehydrogenase 10000U / L

[0059] Formate dehydrogenase 12000U / L

[0060] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0061] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured formaldehyde sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is positive reaction (rising reaction), the delay time is about 0 minutes,...

Embodiment 3

[0064]The formaldehyde determination reagent of the present embodiment is three reagents, comprises:

[0065] Reagent 1

[0066] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0067] Stabilizer 50mmol / L

[0068] Coenzyme 3mmol / L

[0069] Reagent 2

[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 500mmol / L

[0072] Formate dehydrogenase 12000U / L

[0073] Reagent 3

[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0075] Stabilizer 500mmol / L

[0076] Formaldehyde dehydrogenase 10000U / L

[0077] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0078] When measuring formaldehyde concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, formaldehyde sample ...

technical field

[0001] The invention relates to a reagent (box) for measuring formaldehyde, and at the same time, the invention also relates to a method for measuring formaldehyde concentration, which belongs to the technical field of food / environmental inspection and determination. Background technique

[0002] The determination methods of formaldehyde mainly include spectrophotometry, chromatography, electrochemical method, chemical titration, etc.:

[0003] The main methods for spectrophotometric determination are acetylacetone method, chromic acid method, MBTH method, fumagenta method, AHMT method and so on. The advantage of spectrophotometry is that it is easy to operate; the disadvantage is that it has low sensitivity, polluted medium, and interference to the measurement.

[0004] Chromatography mainly includes gas chromatography, high performance liquid chromatography, ion chromatography, etc. Chromatography is rarely used directly, and it is generally used in conjun...

Examples

Embodiment 1

[0040] The formaldehyde determination reagent of the present embodiment is a single reagent, comprising:

[0041] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0042] Stabilizer 500mmol / L

[0043] Coenzyme 3mmol / L

[0044] Formaldehyde dehydrogenase 10000U / L

[0045] Formate dehydrogenase 12000U / L

[0046] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0047] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured formaldehyde sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0048] After adding the sample and reagent, let them mix and rea...

Embodiment 2

[0050] The formaldehyde determination reagent of the present embodiment is double reagent, comprises:

[0051] Reagent 1

[0052] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0053] Stabilizer 50mmol / L

[0054] Coenzyme 3mmol / L

[0055] Reagent 2

[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Formaldehyde dehydrogenase 10000U / L

[0059] Formate dehydrogenase 12000U / L

[0060] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0061] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured formaldehyde sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is positive reaction (rising reaction), the delay time is about 0 minutes,...

Embodiment 3

[0064]The formaldehyde determination reagent of the present embodiment is three reagents, comprises:

[0065] Reagent 1

[0066] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0067] Stabilizer 50mmol / L

[0068] Coenzyme 3mmol / L

[0069] Reagent 2

[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 500mmol / L

[0072] Formate dehydrogenase 12000U / L

[0073] Reagent 3

[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0075] Stabilizer 500mmol / L

[0076] Formaldehyde dehydrogenase 10000U / L

[0077] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0078] When measuring formaldehyde concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, formaldehyde sample ...