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Reagent (kit) for measuring formaldehyde and method for measuring concentration of formaldehyde

A concentration determination and reagent technology, applied in the field of formaldehyde concentration determination and formaldehyde determination reagents, can solve the problems of poor method selectivity, large consumption of reagents, and complicated operation.

Inactive Publication Date: 2010-08-04
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatographic method is simple, fast and direct, but has disadvantages such as cumbersome operation, large consumption of reagents and poor method selectivity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The formaldehyde determination reagent of this embodiment is a single reagent, including:

[0046] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 500mmol / L

[0048] Coenzyme 3mmol / L

[0049] Formaldehyde dehydrogenase 10000U / L

[0050] Lactate aldolase 12000U / L

[0051] Lactate dehydrogenase 12000U / L

[0052] Pyruvate dehydrogenase 12000U / L

[0053] Acetaldehyde 9mmol / L

[0054] Coenzyme A 3mmol / L

[0055] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, pure water is added and reconstituted before use.

[0056] Set on the automatic biochemical analyzer: the temperature is 37°C, the reaction time is 10 minutes, the initial absorbance is less than or equal to 0.1, the main test wavelength is 340nm, the test sub-wavelength is 405nm, the volume ratio of the tested formaldehyde sample to the reagent is 1 / 25, and the reaction The direction...

Embodiment 2

[0059] The formaldehyde determination reagent of the present embodiment is a dual reagent, including:

[0060] Reagent 1

[0061] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0062] Stabilizer 50mmol / L

[0063] Coenzyme 3mmol / L

[0064] Acetaldehyde 9mmol / L

[0065] Coenzyme A 3mmol / L

[0066] Reagent 2

[0067] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0068] Stabilizer 50mmol / L

[0069] Formaldehyde dehydrogenase 10000U / L

[0070] Lactate aldolase 12000U / L

[0071] Lactate dehydrogenase 12000U / L

[0072] Pyruvate dehydrogenase 12000U / L

[0073] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0074] Set on the automatic biochemical analyzer: the temperature is 37°C, the reaction time is 10 minutes, the initial absorbance is less than or equal to 0.1, the main test wavelength is 340nm, the test sub-wavelength is 405nm, and t...

Embodiment 3

[0077] The formaldehyde determination reagent of this embodiment is three reagents, including:

[0078] Reagent 1

[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0080] Stabilizer 50mmol / L

[0081] Coenzyme 3mmol / L

[0082] Acetaldehyde 9mmol / L

[0083] Coenzyme A 3mmol / L

[0084] Reagent 2

[0085] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0086] Stabilizer 500mmol / L

[0087] Lactate aldolase 12000U / L

[0088] Lactate dehydrogenase 12000U / L

[0089] Pyruvate dehydrogenase 12000U / L

[0090] Reagent 3

[0091] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0092] Stabilizer 500mmol / L

[0093] Formaldehyde dehydrogenase 10000U / L

[0094] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0095] When measuring the formaldehyde concentration, set on the automatic biochemical analyzer: the temperature...

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Abstract

The invention relates to a reagent (kit) for measuring formaldehyde by using an enzyme triple multiplied method, an enzyme colorimetric method and an enzyme coupling method as well as a method for measuring the concentration of the formaldehyde, and composition and ingredients of the reagent, belonging to the technical field of food / environmental test. The reagent (kit) comprises the main ingredients of buffer solution, coenzyme, acetaldehyde, coenzyme A, formaldehyde dehydrogenase, lactic acid aldolase, lactic dehydrogenase, pyruvate dehydrogenase and stabilizing agent. The concentration of the formaldehyde is measured by mixing a sample and the reagent according to a certain volume ratio to carry out a series of enzymatic reaction, placing the reactant under an ultraviolet / visible light analyzer and detecting the ascending degree of absorbance at a dominant wavelength of 340nm.

Description

technical field [0001] The invention relates to a formaldehyde determination reagent (box), and also relates to a method for determining formaldehyde concentration, belonging to the technical field of food / environmental inspection and determination. Background technique [0002] The main methods for the determination of formaldehyde are spectrophotometry, chromatography, electrochemical method, chemical titration, etc. The main methods of spectrophotometry are acetylacetone method, chromotropic acid method, MBTH method, fuming red method, AHMT method and so on. The advantage of spectrophotometry is that it is easy to operate; the disadvantage is that the sensitivity is low, and there are polluting media, which interfere with the determination. [0003] Chromatography mainly includes gas chromatography, high performance liquid chromatography, ion chromatography, etc. The direct use of chromatography is less, and it is generally used in conjunction with other analytical instr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N35/00C12Q1/26C12Q1/32C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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