Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Homocysteine diagnosis/determination reagent (kit) and homocysteine concentration determination method

A homocysteine ​​and reagent technology, which is applied in the field of medical testing and determination, can solve the problems of large variation of test data, complicated operation, and long test time.

Inactive Publication Date: 2010-06-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it has the disadvantages of complicated operation, long test time and large variation of test data.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0042] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0043] Stabilizer 500mmol / L

[0044] Coenzyme 3mmol / L

[0045] Methionine synthase 10000U / L

[0046] Dihydrofolate reductase 12000U / L

[0047]5-Methyltetrahydrofolate 6mmol / L

[0048] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0049] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, volume ratio of tested homocysteine ​​sample to reagent is 1 / 25, the reaction direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0050] After adding the samples and reagents, l...

Embodiment 2

[0052] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a dual reagent, including:

[0053] Reagent 1

[0054] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0055] Stabilizer 50mmol / L

[0056] Coenzyme 3mmol / L

[0057] 5-Methyltetrahydrofolate 6mmol / L

[0058] Reagent 2

[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0060] Stabilizer 50mmol / L

[0061] Methionine synthase 10000U / L

[0062] Dihydrofolate reductase 12000U / L

[0063] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0064] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, tested homocysteine ​​sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the reaction direction is positive reaction (rising reaction), the delay ...

Embodiment 3

[0067] The homocysteine ​​diagnosis / measurement reagents of this embodiment are three reagents, including:

[0068] Reagent 1

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 50mmol / L

[0071] Coenzyme 3mmol / L

[0072] 5-Methyltetrahydrofolate 6mmol / L

[0073] Reagent 2

[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0075] Stabilizer 500mmol / L

[0076] Dihydrofolate reductase 12000U / L

[0077] Reagent 3

[0078] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0079] Stabilizer 500mmol / L

[0080] Methionine synthase 10000U / L

[0081] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0082] When measuring the concentration of homocysteine, set it on the automatic biochemical analyzer: temperature 37 ° C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340n...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a homocysteine diagnosis / determination reagent (kit) using enzyme-colorimetry and enzyme-linked method technology. Meanwhile, the invention also relates to a homocysteine concentration determination method, reagent composition and component, which belongs to the technical filed of medical test and determination. The reagent (box) of the invention mainly includes: buffer solution, coenzyme, 5-methyltetrahydrofolate, methionine synzyme, dihydrofolate reductase and stabilizing agent; samples and reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions; then the reactants are arranged under an ultraviolet / visible light analyzer to detect the increasing degree of absorbance at the 340nm position of a dominant wave so as to measure and calculate the concentration of the homocysteine.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnosis / measurement reagent (box), and at the same time, the invention also relates to a method for measuring the homocysteine ​​concentration, which belongs to the technical field of medical examination and measurement. Background technique [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] 1. High-performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. In clinical application, it is gradually adopted by enzyme-linked immunoassay, Fluorescence polarization analysis and enzymatic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com