Glycine diagnosis/measuring reagent kit and glycine concentration determination method

A technology for concentration determination and glycine transaminase, which is applied in biological testing, microbial measurement/inspection, biochemical equipment and methods, etc., can solve problems affecting health, nutritional imbalance, etc.

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the human body consumes too much glycine, not only can it not be absorbed and utilized by the human body, but it will also break the body's absorption balance of amino acids and affect the absorption of other amino acids, resulting in nutritional imbalance and affecting health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The glycine diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0043] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0044] Stabilizer 500mmol / L

[0045] Coenzyme 3mmol / L

[0046] Glycine aminotransferase 10000U / L

[0047] Glutamate dehydrogenase 12000U / L

[0048] Guanosine monophosphate reductase 12000U / L

[0049] 2-ketoglutaric acid 12mmol / L

[0050] Guanosine Monophosphate 12mmol / L

[0051] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0052] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested glycine sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is abou...

Embodiment 2

[0055] The glycine diagnosis / measurement reagent of this embodiment is a double reagent, comprising:

[0056] Reagent 1

[0057] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0058]Stabilizer 50mmol / L

[0059] Coenzyme 3mmol / L

[0060] 2-ketoglutaric acid 12mmol / L

[0061] Guanosine Monophosphate 12mmol / L

[0062] Reagent 2

[0063] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0064] Stabilizer 50mmol / L

[0065] Glycine aminotransferase 10000U / L

[0066] Glutamate dehydrogenase 12000U / L

[0067] Guanosine monophosphate reductase 12000U / L

[0068] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0069] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested glycine sample to reagent ...

Embodiment 3

[0072] The glycine diagnosis / measurement reagent of this embodiment is three reagents, comprising:

[0073] Reagent 1

[0074] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0075] Stabilizer 50mmol / L

[0076] Coenzyme 3mmol / L

[0077] 2-ketoglutaric acid 12mmol / L

[0078] Guanosine Monophosphate 12mmol / L

[0079] Reagent 2

[0080] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 500mmol / L

[0082] Glutamate dehydrogenase 12000U / L

[0083] Guanosine monophosphate reductase 12000U / L

[0084] Reagent 3

[0085] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0086] Stabilizer 500mmol / L

[0087] Glycine aminotransferase 10000U / L

[0088] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0089] When measuring the concentration of glycine, set on the automatic biochemical analyzer: temperature 37°C...

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PUM

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Abstract

The invention relates to a kit for diagnosing/measuring glycine by utilizing the technologies of the enzymatic doubling amplification method, the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the glycine, and belongs to the technical field of medical/food inspection and measurement. The main components of the kit include a buffer solution, coenzyme, a 2-ketoglutaric acid, guanosine monophosphate, glycine aminotransferase, glutamate dehydrogenase, guanosine monophosphate reduced enzyme and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree/velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the glycine.

Description

technical field [0001] The invention relates to a glycine diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring glycine concentration, which belongs to the technical field of medicine / food inspection and determination. Background technique [0002] Glycine is an important fine chemical intermediate, widely used in pesticide, medicine, food, chemical fertilizer, feed and other industries. According to the preparation process of glycine and the purity of the product, it can be divided into four specifications: food grade, pharmaceutical grade, feed grade and industrial grade. [0003] Among the more than 20 kinds of amino acids, glycine is the simplest one. It is a component of protein and is a non-essential amino acid. The "Hygienic Standards for the Use of Food Additives" stipulates that glycine is allowed to be used in seasonings and soybean milk, and the maximum usage is 1g / kg. The results of a special supervision and inspe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/68C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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