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Kalium ion diagnosis/measuring reagent kit and kalium ion concentration determination method

A kit and ion technology, applied in the field of determination of potassium concentration, potassium diagnosis/measurement kit, can solve the problem of high electrode cost

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the ion selective electrode method is widely used, but the cost of the electrode is expensive.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0050] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0051]Stabilizer 500mmol / L

[0052] Reduced coenzyme 0.25mmol / L

[0053] Pyruvate kinase 6000U / L

[0054] Pyruvate oxidase 8000U / L

[0055] NADH peroxidase 10000U / L

[0056] Adenosine diphosphate 3mmol / L

[0057] Phosphoenolpyruvate 3mmol / L

[0058] Coenzyme A 4mmol / L

[0059] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0060] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested potassium (ion) sample to the reagent is 1 / 25, the reaction direction is negative reaction (downward reaction)...

Embodiment 2

[0063] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0064] Reagent 1

[0065] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 50mmol / L

[0067] Reduced coenzyme 0.25mmol / L

[0068] Adenosine diphosphate 3mmol / L

[0069] Phosphoenolpyruvate 3mmol / L

[0070] Coenzyme A 4mmol / L

[0071] Reagent 2

[0072] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0073] Stabilizer 500mmol / L

[0074] Pyruvate kinase 6000U / L

[0075] Pyruvate oxidase 8000U / L

[0076] NADH peroxidase 10000U / L

[0077] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0078] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, tested potassium (ion) sample and reagent 1, ...

Embodiment 3

[0081] The potassium (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0082] Reagent 1

[0083] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0084] Stabilizer 50mmol / L

[0085] Reduced coenzyme 0.25mmol / L

[0086] Adenosine diphosphate 3mmol / L

[0087] Phosphoenolpyruvate 3mmol / L

[0088] Coenzyme A 4mmol / L

[0089] Reagent 2

[0090] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0091] Stabilizer 500mmol / L

[0092] Pyruvate oxidase 8000U / L

[0093] NADH peroxidase 10000U / L

[0094] Reagent 3

[0095] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0096] Stabilizer 500mmol / L

[0097] Pyruvate kinase 6000U / L

[0098] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0099] When measuring potassium (ion) concentration, set on the automatic biochemical analyzer: ...

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Abstract

The invention relates to a kit for diagnosing / measuring potassium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the potassium (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, adenosine diphosphate, a phosphoenolpyruvic acid, coenzyme A, pyruvate kinase, pyruvate oxidase, NADH peroxydase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of potassium (ions).

Description

technical field [0001] The invention relates to a potassium (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring potassium (ion) concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] Glomerular disease, renal insufficiency, diabetic ketosis, adrenal insufficiency, etc. When the serum potassium is as high as 7.5mmol / L or above, the patient will experience arrhythmia, and appropriate treatment should be considered. When serum potassium exceeds 10mmol / L, ventricular fibrillation can occur, cardiac arrest and death. High potassium stops the heart in diastole. [0003] At present, the commonly used methods for potassium determination are: isotope dilution mass spectrometry, neutron activation method, flame photometer method, chemical determination method, ion selective electrode method, atomic spectrophotometry, enzyme kinetics method. ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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