Kalium ion diagnosis/measuring reagent kit and kalium ion concentration determination method

A kit and ion technology, applied in the field of determination of potassium concentration, potassium diagnosis/measurement kit, can solve the problem of high electrode cost

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the ion selective electrode method is wid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0050] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0051]Stabilizer 500mmol / L

[0052] Reduced coenzyme 0.25mmol / L

[0053] Pyruvate kinase 6000U / L

[0054] Pyruvate oxidase 8000U / L

[0055] NADH peroxidase 10000U / L

[0056] Adenosine diphosphate 3mmol / L

[0057] Phosphoenolpyruvate 3mmol / L

[0058] Coenzyme A 4mmol / L

[0059] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0060] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested potassium (ion) sample to the reagent is 1 / 25, the reaction direction is negative reaction (downward reaction)...

Embodiment 2

[0063] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0064] Reagent 1

[0065] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 50mmol / L

[0067] Reduced coenzyme 0.25mmol / L

[0068] Adenosine diphosphate 3mmol / L

[0069] Phosphoenolpyruvate 3mmol / L

[0070] Coenzyme A 4mmol / L

[0071] Reagent 2

[0072] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0073] Stabilizer 500mmol / L

[0074] Pyruvate kinase 6000U / L

[0075] Pyruvate oxidase 8000U / L

[0076] NADH peroxidase 10000U / L

[0077] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0078] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, tested potassium (ion) sample and reagent 1, ...

Embodiment 3

[0081] The potassium (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0082] Reagent 1

[0083] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0084] Stabilizer 50mmol / L

[0085] Reduced coenzyme 0.25mmol / L

[0086] Adenosine diphosphate 3mmol / L

[0087] Phosphoenolpyruvate 3mmol / L

[0088] Coenzyme A 4mmol / L

[0089] Reagent 2

[0090] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0091] Stabilizer 500mmol / L

[0092] Pyruvate oxidase 8000U / L

[0093] NADH peroxidase 10000U / L

[0094] Reagent 3

[0095] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0096] Stabilizer 500mmol / L

[0097] Pyruvate kinase 6000U / L

[0098] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0099] When measuring potassium (ion) concentration, set on the automatic biochemical analyzer: ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a kit for diagnosing/measuring potassium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the potassium (ions), and belongs to the technical field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, adenosine diphosphate, a phosphoenolpyruvic acid, coenzyme A, pyruvate kinase, pyruvate oxidase, NADH peroxydase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of potassium (ions).

Description

technical field [0001] The invention relates to a potassium (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring potassium (ion) concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] Glomerular disease, renal insufficiency, diabetic ketosis, adrenal insufficiency, etc. When the serum potassium is as high as 7.5mmol / L or above, the patient will experience arrhythmia, and appropriate treatment should be considered. When serum potassium exceeds 10mmol / L, ventricular fibrillation can occur, cardiac arrest and death. High potassium stops the heart in diastole. [0003] At present, the commonly used methods for potassium determination are: isotope dilution mass spectrometry, neutron activation method, flame photometer method, chemical determination method, ion selective electrode method, atomic spectrophotometry, enzyme kinetics method. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap