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Carbon dioxide diagnosis/ determination reagent kit and carbon dioxide concentration determination method

A technology of carbon dioxide and kits, which is applied in the field of carbon dioxide diagnosis/measurement kits and carbon dioxide concentration measurement, and can solve problems such as lack of experimental research

Inactive Publication Date: 2008-04-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The above patents have nothing to do with enzymatic determination, which shows from one aspect that domestic experimental research in this area is very lacking

Method used

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  • Carbon dioxide diagnosis/ determination reagent kit and carbon dioxide concentration determination method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The carbon dioxide diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0051]Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0052] Stabilizer 50mmol / L

[0053] Coenzyme 3mmol / L

[0054] Coenzyme A 6mmol / L

[0055] Acetic acid 20mmol / L

[0056] Ferrocytochrome b1 9mmol / L

[0057] Cytochrome-dependent pyruvate dehydrogenase 6000U / L

[0058] Coenzyme-dependent pyruvate dehydrogenase 6000U / L

[0059] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0060] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the measured carbon dioxide sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is...

Embodiment 2

[0063] The carbon dioxide diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0064] Reagent 1

[0065] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 50mmol / L

[0067] Coenzyme 3mmol / L

[0068] Coenzyme A 8mmol / L

[0069] Acetic acid 30mmol / L

[0070] Ferrocytochrome b1 12mmol / L

[0071] Reagent 2

[0072] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0073] Stabilizer 50mmol / L

[0074] Cytochrome-dependent pyruvate dehydrogenase 12000U / L

[0075] Coenzyme-dependent pyruvate dehydrogenase 12000U / L

[0076] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0077] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured carbon dioxide sa...

Embodiment 3

[0080] The carbon dioxide diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0081] Reagent 1

[0082] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0083] Stabilizer 50mmol / L

[0084] Coenzyme 3mmol / L

[0085] Coenzyme A 12mmol / L

[0086] Reagent 2

[0087] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0088] Acetic acid 10mmol / L

[0089] Ferrocytochrome b1 6mmol / L

[0090] Reagent 3

[0091] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0092] Stabilizer 50mmol / L

[0093] Cytochrome-dependent pyruvate dehydrogenase 8000U / L

[0094] Coenzyme-dependent pyruvate dehydrogenase 8000U / L

[0095] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0096] When measuring carbon dioxide concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 m...

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Abstract

The invention relates to a carbon dioxide diagnosing / measuring reagent box which utilizes the enzyme circulation enlarging method, the enzyme contrasting color method and the enzyme jointing method techniques, and simultaneously the invention also relates to a method principle of the carbon dioxide concentration measuring, and the composition as well as the components of reagent, and belongs to the technical field of the medicine / food / environment test. The main components of the reagent box of the invention include cushion liquid, coenzyme, acetic acid, ferricytochrome b1, coenzyme A, pyruvate dehydrogenase with cytochrom dependency, pyruvate dehydrogenase with coenzyme dependency and stabilizing agent and the reagent box generates a series of enzyme promoting reactions trough the mixing of the samples and the reagent as a certain cubage rate, and then the reactants are positioned under a ultraviolet / visible light analyzer which tests the ascending speed of the absorbency at the main wave length 340nm, thereby measuring the concentration size of the carbon dioxide. The invention can absolutely get needed measuring result through the ultraviolet / visible light analyzer.

Description

technical field [0001] The invention relates to a carbon dioxide diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring carbon dioxide concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] The content of carbon dioxide in the blood plays an important role in the regulation of acid-base balance in the human body. The constant blood pH is an essential condition for maintaining life activities. Among the various buffer systems in blood plasma, the bicarbonate buffer system is the most important, which directly affects the pH of blood. [0003] Changes in the content of carbon dioxide in the blood mainly reflect metabolic acid-base balance disorders. In pure metabolic acid or alkalosis, blood bicarbonate [HCO 3 - ] decreased or increased, the total carbon dioxide also decreased or increased. In simple respiratory acid or alkalosis, blood bicar...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/78G01N21/25C12Q1/32
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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