Reagent (kit) for diagnosing/determining amino acid and method for determining concentration of amino acid
An amino acid and reagent technology, applied in the field of amino acid concentration determination and amino acid diagnosis/determination reagents, can solve the problems of high cost of instruments and equipment, complicated operation methods, poor specificity, etc.
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Embodiment 1
[0040] The amino acid diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0041] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0042] Stabilizer 500mmol / L
[0043] Coenzyme 3mmol / L
[0044] Amino acid oxidase 10000U / L
[0045] Galactose oxidase 12000U / L
[0046] Galactose dehydrogenase 12000U / L
[0047] Galactose Hexose Dialdose 12mmol / L
[0048] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0049] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the amino acid sample to be tested is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.
[0050] Af...
Embodiment 2
[0052] The amino acid diagnosis / measurement reagent of this embodiment is a double reagent, including:
[0053] Reagent 1
[0054] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0055] Stabilizer 50mmol / L
[0056] Coenzyme 3mmol / L
[0057] Galactose Hexose Dialdose 12mmol / L
[0058] Reagent 2
[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0060] Stabilizer 50mmol / L
[0061] Amino acid oxidase 10000U / L
[0062] Galactose oxidase 12000U / L
[0063] Galactose dehydrogenase 12000U / L
[0064]After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0065] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the amino acid sample to be tested to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direct...
Embodiment 3
[0068] The amino acid diagnosis / measurement reagents of this embodiment are three reagents, including:
[0069] Reagent 1
[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0071] Stabilizer 50mmol / L
[0072] Coenzyme 3mmol / L
[0073] Galactose Hexose Dialdose 12mmol / L
[0074] Reagent 2
[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0076] Stabilizer 500mmol / L
[0077] Galactose oxidase 12000U / L
[0078] Galactose dehydrogenase 12000U / L
[0079] Reagent 3
[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0081] Stabilizer 500mmol / L
[0082] Amino acid oxidase 10000U / L
[0083] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0084] When measuring amino acid concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test ...
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