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Urine galactose test reagent kit

A galactose and kit technology, applied in the field of in vitro diagnostic kits, can solve problems such as inability to completely remove interfering substances in urine and inability to carry out enzymatic reactions, and achieve the effects of avoiding improper storage, easy operation and improving accuracy

Inactive Publication Date: 2006-08-02
北京中生金域诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the subjects ingest a large amount of fruit, or ingest Vit C drugs, or other reasons cause the electrolyte concentration in urine to be too high, the interfering substances in urine cannot be completely removed, so that the enzymatic reaction cannot be carried out.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0040] choose figure 1 Shown urine purification device 10, this urine purification device is a kind of titration container, and selective specific adsorbent is housed in its titration container 3; An adsorbent composed of aluminum, 0.36g of purified activated carbon and 5mg of sucrose; wherein the purified activated carbon was soaked in 10mmol / L sodium acetate buffer solution with pH=3; the selective specific adsorbent was activated by baking at 150°C for 36 hours; The activated selective specific adsorbent can completely absorb and remove interfering substances in urine such as bilirubin, vitamin C, creatinine, etc., but does not adsorb galactose;

[0041] Prepare galactose oxidase pads:

[0042] Whatman's 3MM grade chromatographic paper was used as the carrier, cut into φ4.5mm discs, and the chromatographic paper carrier was made of 1.8×10 5 U / m 2 Galactose oxidase, pH=6.8, soaked in an enzyme solution composed of 100mmol / L sodium phosphate buffer and 10% trehalose by mas...

Embodiment 2

[0055] choose figure 1 Shown urine purification device 10, this urine purification device is a kind of titration container, and selective specific adsorbent is housed in its titration container 3; An adsorbent composed of aluminum, 0.45g of purified activated carbon and 7mg of fructose; wherein the purified activated carbon was soaked in 10mmol / L potassium acetate buffer solution with pH=3; the selective specific adsorbent was activated by baking at 70°C for 72 hours; The activated selective specific adsorbent can completely absorb and remove interfering substances in urine such as bilirubin, vitamin C, creatinine, etc., but does not adsorb galactose;

[0056] Prepare galactose oxidase pads:

[0057] Whatman's 3MM grade chromatographic paper is used as the carrier, cut into φ4.5mm discs, and the chromatographic paper carrier is made of 2.0×10 5 U / m 2 Galactose oxidase, pH=7.0, soaked in an enzyme solution consisting of 100mmol / L phosphate buffer and 13% trehalose by mass fo...

Embodiment 3

[0066] choose figure 1 Shown urine purification device 10, this urine purification device is a kind of titration container, and selective specific adsorbent is housed in its titration container 3; An adsorbent composed of aluminum, 0.8g of purified activated carbon and 15mg of glucose; wherein the purified activated carbon is soaked in 10mmol / L acetate buffer solution with pH=6.5; the selective specific adsorbent is activated by baking at 250°C for 4 hours; The activated selective specific adsorbent can completely absorb and remove interfering substances in urine such as bilirubin, vitamin C, creatinine, etc., but does not adsorb galactose;

[0067] Prepare galactose oxidase pads:

[0068] Whatman's 3MM grade chromatographic paper was used as the carrier, cut into φ4.5mm discs, and the chromatographic paper carrier was made of 2.5×10 5 U / m 2 Galactose oxidase, pH=7.0, soaked in an enzyme solution consisting of 100 mmol / L phosphate buffer and 20% trehalose by mass for 2 hour...

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Abstract

The present invention relates to a urinary galactose determination kit. It includes urine purification device, dry chemical reaction device and standard liquor. The urine purification device is a titration type container in which a selective specific adsorbent is held. Said selective specific adsorbent is formed from aluminium oxide, purification active carbon and galactose adsorption agonist. Besides, said invention also provides the concrete structure of said dry chemical reaction device and concrete composition of said standard liquor. Said kit can completely remove interference substance from urine, so that it can greatly raise accuracy of determination.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic kits, and in particular relates to a urine galactose determination kit, which semi-quantitatively detects the concentration of galactose in human urine by a dry chemical method, and screens or assists in the diagnosis of lactose malabsorption and lactose insufficiency. Tolerance. Background technique [0002] Many people have symptoms such as abdominal discomfort, bloating, gas, abdominal pain, and even diarrhea after drinking milk. This phenomenon is called lactose intolerance in medicine. A large number of studies have confirmed that only about 30% of people suffering from lactose intolerance have the above-mentioned symptoms. It is called lactose intolerance in medicine, and most of them are recessive intolerance without any symptoms and only with changes in human biochemical indicators. Medically known as lactose malabsorption or lactase deficiency. [0003] According to statistics, the pr...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/25G01N33/66
Inventor 王加义
Owner 北京中生金域诊断技术股份有限公司
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