Screening methods, devices and kits for detecting mucous membrane carbohydrates and related conditions

A technology of carbohydrates and screening devices, applied in biochemical equipment and methods, measuring devices, biological tests, etc., can solve the problems of false negatives due to errors

Pending Publication Date: 2022-07-22
阿布卡拉姆·穆罕默德·沙姆斯丁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the test kit includes multiple liquid components, and the test method requires multiple steps, which will produce false negatives through sampling or procedural errors

Method used

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  • Screening methods, devices and kits for detecting mucous membrane carbohydrates and related conditions
  • Screening methods, devices and kits for detecting mucous membrane carbohydrates and related conditions

Examples

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Embodiment 1

[0091] Example 1 - Mucosal scraping samples were collected from subjects. The scraped samples were mixed with distilled or reverse osmosis water and added to a galactose oxidase solution at a concentration of 100 U / mL. The sample was left in contact with galactose oxidase for 10 minutes before adding the sample to a test strip with pre-embedded Schiff's reagent. The test strip is rinsed with additional distilled water, then the Schiff's reagent is activated and left in contact with the sample for 1 minute, after which the test strip is rinsed with water and dried in the open air or in an oven. figure 2 A description of test sample processing is provided. A change in color (from white or colorless to magenta) indicates the presence of carbohydrate markers.

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Abstract

The present disclosure relates to screening test methods, devices, and kits for mucosal carbohydrates and related conditions, including cancerous and pre-cancerous conditions. In particular, the method uses a galactose oxidase reagent and a Schiff reagent for testing abnormal carbohydrates in mucus or bodily fluids. The screening test methods, devices, and kits provide improved accuracy and minimize processing procedures. The present disclosure also relates to the use of the device or kit in a health care facility for the preliminary evaluation of cancerous and pre-cancerous conditions.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS [0002] This application claims priority to and the benefit of US Provisional Application No. 62 / 893,477, filed August 29, 2019, and US Non-Provisional Patent Application No. 16 / 936,998, filed July 23, 2020, the entire contents of which are hereby incorporated by reference in their entirety Incorporated herein. technical field [0003] The present disclosure generally relates to screening test methods, devices and kits for the detection of mucosal carbohydrates and related disorders, including but not limited to cancerous and precancerous disorders. Specifically, the method uses the enzymes galactose oxidase and Schiffs Reagent to test for abnormal carbohydrates in mucus or body fluids. The present disclosure also relates to the use of the device or kit in a healthcare facility for the initial assessment of abnormal carbohydrates and related disorders. Background technique [0004] Early detection and prevention remain the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/26G01N33/574G01N33/558G01N33/52
CPCC12Q1/26G01N33/57484G01N33/57469G01N33/523G01N33/558G01N2333/902G01N2333/904G01N2400/00G01N33/54388G01N33/574
Inventor 阿布卡拉姆·穆罕默德·沙姆斯丁
Owner 阿布卡拉姆·穆罕默德·沙姆斯丁
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