28 results about "Glucaric Acid" patented technology

The invention belongs to the technical field of gene engineering, and discloses recombinant yarrowia lipolytica as well as a construction method and application thereof. The recombinant yarrowia lipolytica PGA1 is obtained by carrying out heterologous expression on an arabidopsis thaliana inositol oxygenase gene and a pseudomonas syringae uronic acid dehydrogenase gene in the yarrowia lipolytica by utilizing a genetic engineering technical means. The recombinant yarrowia lipolytica PGA10 capable of efficiently synthesizing glucaric acid (GA) is finally obtained by further modifying six genes of related metabolic pathways of the recombinant yarrowia lipolytica PGA1. The GA yield of the PGA10 reaches 2233.24 mg / L and is 66.27 times that of PGA1, and the effect is remarkable. The invention not only constructs a GA synthesis method, but also defines the influence of related genes such as opi1 on GA synthesis, and provides reference for the research of heterologous synthesis of GA by microorganisms.

The invention belongs to the field of quality analysis and control of traditional Chinese medicines, and discloses a method for determining the content of carpesium abrotanoides components, which comprises the following steps: by taking isochlorogenic acid A as an internal reference substance, calculating the content of chlorogenic acid, caffeic acid, galuteolin, 11 (13)-dehydroaxillary Ivalin, isochlorogenic acid C, 2, 3, 4, 5-tetramethyl-1, 3, 4, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5- According to the method, the content of 10 components of carabrone is rapidly and accurately detected according to the relative correction factors of 2, 3, 4, 5-tetracaffeoyl-D-glucaric acid, 2-deoxy-4-surface-gaillanin, carabrone and dellelactone, and the problem that the detection cost is high due to the fact that many reference substances are needed, are not easy to obtain and are high in price in carabrone content determination is solved.

A method of preparing glucaric acid comprising contacting D-glucose and oxygen with a first catalyst composition comprising a first copper radical oxidase, a single electron oxidizer, and a small molecule activator under conditions suitable for formation of glucodialdose; and contacting glucodialdose and oxygen with a second catalyst composition comprising a second copper radical oxidase, a single electron oxidizer and a small molecule activator under conditions suitable for the formation of a product mixture comprising glucaric acid or salts thereof. A method comprising contacting a sugar with a catalyst composition comprising an oxidoreductase, a single electron oxidizer and a small molecule activator under conditions suitable for the formation of one or more oxidized sugar oxidation products comprising glucaric acid wherein the oxidoreductase comprises at least two copper radical oxidases, at least two mutated copper radical oxidases or combinations thereof.

The invention discloses a monatomic loaded lignin carbon-based photocatalyst as well as a preparation method and application thereof. The preparation method of the photocatalyst comprises the following steps: S1, grinding and blending lignin and melamine, then adding the mixture into water, transferring the obtained solution into a hydrothermal reaction container, reacting at 175-195 DEG C for 4-10 hours, and after the reaction is finished, cooling at room temperature, washing and centrifugally separating to obtain a solid mixture; s2, dispersing the solid mixture in an aqueous solution containing metal salt, stirring at room temperature, and drying after the reaction is finished; and S3, putting the dried solid into a container, putting the container into a pyrolysis reactor, heating to 800-1000 DEG C in an argon atmosphere, pyrolyzing for 2-6 hours, and naturally cooling to obtain the monatomic loaded lignin carbon-based photocatalyst. The monatomic loaded lignin carbon-based photocatalyst provided by the invention can efficiently catalyze glucose to synthesize glucaric acid under mild conditions, and the obtained target product is relatively high in yield and easy to separate, and has a very good industrial application prospect.

The invention discloses a fermentation process for high-yield production of glucaric acid by saccharomyces cerevisiae engineering bacteria. According to the process, a fermentation culture medium is improved and dynamically regulated; the yield of glucaric acid in a 50 L fermentation tank reaches 18 g / L, the production cost is greatly reduced, and industrial production is facilitated.

A polymeric material comprising a base monomer and a multifunctional biobased molecule wherein the multifunctional biobased molecule is a glucose derivative. A polymeric material comprising a base polymer and a polymeric additive comprising glucaric acid, glucodialdose, gluconic acid, erythorbic acid, 2-ketoglucose, lactic acid, adipic acid, sorbitol, glycerol, mannitol, lactones thereof, salts thereof, or combinations thereof wherein the additive when introduced to a base polymer forms a modified polymer wherein the modified polymer has a tensile strength that is increased by from about 10% to about 100% wherein compared to the base polymer.

Provided is a biocatalytic method to synthesize L-guluronic acid and glucaric acid. The technical solution is as follow: 1) Firstly, developed is an enzyme-catalyzed route for synthesizing L-guluronic acid by GOase. 2) Secondly, developed is a biocatalytic route for the synthesis of glucaric acid from L-guluronic acid, catalyzed by uronate dehydrogenase (UDH).

A method of producing glucaric acid by electrochemical treatment includes (a) providing a reactor including a first electrode-containing region and a second electrode-containing region separated by an electrolyte membrane, adding a glucose mixture to any one region of the reactor, and adding an electrolyte solution to both regions of the reactor. The method also includes (b) producing glucaric acid by oxidizing glucose by applying a voltage between the first electrode and the second electrode in the reactor subjected to step (a). The electrode in the region to which the glucose mixture is added includes a catalyst containing tantalum.

The application discloses a method for separating glucaric acid by using a bimodal pore amphoteric post-crosslinked resin. 2 The bimodal pore amphoteric post-crosslinked resin mainly comprises a precursor resin with a mesoporous structure, and the pore diameter of the resin is cut to form micropores and mesopores through a Friedel-Crafts alkylation reaction. 2 The specific surface area of the bimodal pore amphoteric post-crosslinked resin is 300-800 m 2 / g; the specific surface area of the micropores is 150-500 m / g; and the specific surface area of the mesopores is 100-300 m / g. The bimodal pore amphoteric post-crosslinked resin prepared by the application has a bimodal pore distribution pore structure, a large adsorption capacity for glucaric acid, high selectivity, and is easy to elute after adsorption by using hot water, and can be used as a new type of adsorption medium for separating glucaric acid from a fermentation broth.

The application discloses application of a diphenyl ether compound in preparation of a beta-glucuronidase inhibitor. The diphenyl ether compound is obtained from a fungus Aspergillus versicolor ZJUTE2 through a series of processes such as organic solvent extraction and chromatographic column separation, simple extraction and separation, and has the advantages of simple preparation method, rapidness, high purity of obtained compound and the like; the diphenyl ether compound extracted by the application has good beta-glucuronidase inhibitory activity, and an IC 50 value of 56.59+ / -1.79 muM, is equivalent to a positive drug D-glucaric acid 1,4-lactone, and can be used for treating drug-induced diarrhea caused by irinotecan or non-steroidal anti-inflammatory drugs. In addition, development of structure optimization based on the compound as a lead is of great significance for research and development of a novel drug for treating drug-induced diarrhea.

To provide processes for preparing glucaric acid and / or lactones thereof.SOLUTION: Various processes for preparing aldaric acids, aldonic acids, uronic acids, and / or lactones thereof are described. For example, processes for preparing a C2-C7 aldaric acid and / or lactones thereof by the catalytic oxidation of a C2-C7 aldonic acid and / or lactones thereof and / or a C2-C7 aldose are described.SELECTED DRAWING: None

A method for the production of 2,5-furandicarboxylic acid (FDCA) comprising contacting glucose with a catalyst composition under conditions suitable for the formation of glucarate; acidifying glucarate to form glucaric acid; dehydrating glucaric acid to form FDCA; and recovering FDCA. A method for the production of 2,5-furan dicarboxylic acid dimethyl ester (FDME) comprising contacting glucose with a catalyst composition under conditions suitable for the formation of glycolaldehyde; contacting glycolaldehyde with a transition metal oxidation catalyst under conditions suitable for the formation of glucaric acid; esterifying glucaric acid to form glucaric acid methyl ester; dehydrating the glucaric acid methyl ester to form FDME; and recovering FDME.

The invention discloses a pharmaceutical composition for treating drug-induced liver injury and application of the pharmaceutical composition, the pharmaceutical composition comprises glucaric acid 1, 4-lactone and N-acetylcysteine, and the mass ratio of the glucaric acid 1, 4-lactone to the N-acetylcysteine is 1: (1-5). The pharmaceutical composition disclosed by the invention can be used for preparing a medicine for treating or preventing the drug-induced liver injury, and the drug-induced liver injury is liver injury caused by excessive acetaminophen or long-term administration. The pharmaceutical composition provides comprehensive liver protection through multi-target cooperation, and has a significant improvement effect on liver injury caused by acetaminophen.

The application belongs to the field of traditional Chinese medicine quality analysis and control, and discloses a determination method of ingredients content of scabiosa comosa, which takes isochlorogenic acid A as an internal reference, calculates the relative correction factors of chlorogenic acid, caffeic acid, jinyingzhi, 11(13)-dehydroxy axillary ivy chrysanthemum, isochlorogenic acid C, 2,3,4,5-tetra caffeoyl-D-glucaric acid, 2-deoxy-4-tablet-Tianrenjuling, scabioside lactone ketone and terlene lactone, quickly and accurately detects the content of 10 kinds of ingredients of scabiosa comosa, and solves the problems of many required control products, difficulty in obtaining and high price in the determination of scabiosa comosa content, and high detection cost.

The invention relates to the technical field of tobacco leaf grade discrimination, in particular to a metabolic marker composition for discriminating the grade of tobacco leaves, a screening method and application. The invention discloses a metabolic marker composition for judging the grade of tobacco leaves. Comprising the following metabolic markers: L-seryl-L-tyrosine, L-tryptophanyl-L-histidine, D-gluconic acid, N-acetylglutamic acid, linoleic acid, alpha-linolenic acid, nicotine, shikimic acid, N-methyl nicotinic acid, O-acetyl-L-carnitine, D-mannose, D-glucaric acid, phosphatidylcholine 36: 1, sucrose, mesaconic acid, fraxin and N-methyltryptamine. By utilizing the 17 metabolic markers, the grade of the tobacco leaves can be effectively distinguished.

The invention belongs to the technical field of medicines, discloses application of D-glucaric acid-1, 4-lactone in preparation of a medicine for relieving intestinal injury caused by sorafenib, and particularly discloses application of D-glucaric acid-1, 4-lactone or pharmaceutically acceptable salt thereof in preparation of a medicine for treating and / or preventing intestinal injury. The invention discloses the application potential of the D-glucaric acid-1, 4-lactone in preparation of medicines for relieving the intestinal injury (especially the intestinal injury caused by molecular targeting medicine sorafenib) for the first time. In the treatment process of sorafenib, patients often need to pause or stop medication due to adverse reactions of gastrointestinal tracts, and the treatment effect of sorafenib is remarkably restricted. Experiments prove that the D-glucaric acid-1, 4-lactone can effectively relieve intestinal injury caused by sorafenib, can relieve mouse intestinal mucosa injury caused by sorafenib, and can relieve intestinal oxidative stress and inflammatory response caused by sorafenib.

The application belongs to the technical field of sodium ion battery negative electrode materials, and discloses a corn powder / glucose acid cross-linking derived hard carbon negative electrode material and a preparation method and application thereof; the preparation method is as follows: corn powder and glucose acid are stirred at a ratio of 10:7-20, the modified corn powder after drying is prepared for carbonization at a low temperature of 500-700 DEG C in an inert gas for 1-3 hours, and then high-temperature carbonization is carried out at 1100-1600 DEG C for 1-3 hours to prepare hard carbon. The preparation method of the green and environment-friendly, low-cost and high-cycle-stability sodium ion battery biomass hard carbon negative electrode material has the characteristics of mass production, controllable hard carbon surface morphology and excellent electrochemical performance.

The invention discloses acid-resistant pichia kuchii and an application of the acid-resistant pichia kuchii in glucaric acid synthesis. The method comprises the following steps: by taking pichia kudriavzevii as a chassis, knocking out pgi and / or pfk genes of an EMP pathway, and integrating inositol-phosphate synthase, inositol monophosphate, thermophilic bacteria source inositol oxygenase and uronic acid dehydrogenase genes containing A39P / H99Y / H234K mutation, so as to construct a multi-copy expression system containing four enzymes. And integrating an exogenous gene expression cassette to an EG site by using a CRISPR / Cas9 system, so as to obtain the pichia pastoris engineering bacterium. The glucaric acid yield of the strain in an experiment can reach 3.22 g / L, and the strain has the advantages of being high in acid resistance, free of acid-base neutralization, capable of utilizing cheap substrates and the like. Through combination of metabolic pathway optimization and acid resistance, an efficient and stable microbial platform is provided for industrial production of glucaric acid.

The invention discloses an anti-saccharification and anti-carbonylation composition as well as a preparation method and application thereof, and the composition comprises quinic acid and 2, 3, 4, 5-tetracaffeoyl-D-glucaric acid. According to the invention, quinic acid and 2, 3, 4, 5-tetracaffeoyl-D-glucaric acid are specifically compounded, and the prepared composition can significantly improve the anti-saccharification effect. And a product containing the composition has obvious effects of resisting saccharification, resisting carbonylation, brightening skin color and improving skin darkness, is good in stability and can be applied to cosmetics with corresponding effects.

A method of producing adipic acid using glucose as a starting material, in which adipic acid is finally obtained via an alkyl adipate intermediate from a glucaric acid potassium salt.

The application discloses a fusion myo-inositol oxygenase for simultaneously improving soluble expression and catalytic activity and application thereof. The fusion myo-inositol oxygenase is composed of an NusA tag fused to the N terminal of myo-inositol oxygenase (Miox). Compared with wild-type Miox, the soluble expression level of the fusion myo-inositol oxygenase is significantly improved, and the specific enzyme activity reaches 0.98 U / mg, which is 48.5% higher than that of the wild type. The recombinant strain carrying the fusion myo-inositol oxygenase is used for biosynthesis of D-glucaric acid, and the yield of shake flask fermentation reaches 286.5 mg / L, which is 31.9% higher than that of the wild type. The fusion myo-inositol oxygenase provided by the application breaks through the limitation that traditional fusion tags are difficult to simultaneously consider expression and activity, and provides an effective technical scheme for efficient biosynthesis of D-glucaric acid.

The invention belongs to the technical field of organic compounds, and particularly relates to a high-foam glycosyl amide organosilicon cationic surfactant as well as a preparation method and application thereof. The preparation method of the surfactant comprises the following steps: taking a renewable resource D-gluconic acid delta-lactone as a raw material, carrying out amidation reaction on the raw material and N, N-dimethyl dipropylene triamine to obtain a glucosamide precursor, reacting the precursor with bromododecane, introducing a hydrophobic chain to obtain an intermediate; and finally, carrying out quaternization reaction on the obtained intermediate and 3-chloropropyl methyl dimethoxy silane / 3-chloropropyl trimethoxy silane, so as to synthesize two kinds of glucosamide organosilicon quaternary ammonium salts. No three wastes are discharged in the synthesis process, and the method is suitable for industrial production; the synthesized surfactant has good foamability, foam stability, biocompatibility, no toxicity, no irritation and bactericidal performance, and can play an important role in the fields of medical treatment, self-cleaning, electronics, energy, agriculture, daily chemical industry and the like in the future.

Described herein are processes and enzymes for the enzymatic production of glucaric acid from glucuronic acid, and more specifically the enzymatic production of substituted glucaric acid from substituted glucuronic acid. Advantageously, the process and enzymes described herein make preferential use substituted glucuronic acid obtained from natural sources, such as underutilized hemicellulose stream from wood and agricultural biorefineries, to produce substantially enantiomerically pure substituted D-glucaric acid.

The application discloses a kind of recombinant escherichia coli and its construction method and the method for producing glucaric acid by metabolic engineering, belong to the field of metabolic engineering.The application realizes the production pathway of glucuronic acid by expressing the myo-inositol-1-phosphate synthase gene (Ino1) and myo-inositol oxidase gene (MIOX) derived from plants in escherichia coli, simultaneously expresses the aldehyde acid dehydrogenase gene (Udh) derived from Agrobacterium tumefaciens, and successfully constructs the synthesis pathway of glucaric acid.The recombinant escherichia coli generates glucaric acid 2.53g / L in LB-G (10g / L glucose is added in LB medium) fermentation medium.The method for fermenting and producing glucaric acid by the recombinant escherichia coli proposed in the application has broad development prospect, and lays the foundation for biosynthesis of glucaric acid.

The invention discloses an application of a glucaric acid 1, 4-lactone and lenvatinib composition in preparation of an STAT3 phosphorylation inhibitor, the pharmaceutical composition comprises an active component A and an active component B, the active component A is D-glucaric acid-1, 4-lactone, and the active component B is lenvatinib or a pharmaceutically acceptable salt thereof; the mass ratio of the D-glucaric acid-1, 4-lactone to the lenvatinib is 1 to (0.1 to 50). The pharmaceutical composition can be used for preparing STAT3 inhibitors, anti-liver cancer drugs and drugs for inhibiting immune escape of liver cancer cells. According to the pharmaceutical composition, the anti-tumor effect of lenvatinib is enhanced by inhibiting STAT3 phosphorylation and PD-L1 protein expression, and a new scheme is provided for solving the drug resistance problem of lenvatinib.

The bioprosthetic valve is treated by a first cross-linking agent and a second cross-linking agent, the first cross-linking agent is L-asparaginic acid, L-glutamic acid, glucaric acid, malic acid or succinic acid, and the second cross-linking agent is chitosan oligosaccharide, L-lysine, arginine or histidine. The invention further provides a preparation method of the bioprosthetic valve, according to the bioprosthetic valve and the preparation method of the bioprosthetic valve, almost non-toxic bio-friendly reagents which are widely applied to food and medicine are adopted, cross-linking is carried out twice in sequence, multi-site stable cross-linking of amino and carboxyl of biological tissue is achieved, the physical performance of materials is improved, and the bioprosthetic valve and the preparation method of the bioprosthetic valve are suitable for large-scale popularization and application. Meanwhile, the calcification problem caused by the toxicity of the cross-linking agent is avoided.

The invention discloses a road guardrail maintenance coating based on an in-situ mineralized chloride ion curing mechanism and a preparation method thereof. Relates to the technical field of concrete protective coatings, and the coating is a two-component system and is composed of an active modified mineral precursor powder material and a functional silicate carrier liquid material. The powder material is prepared from light calcined magnesia, pseudo-boehmite, activated zinc oxide, an amino acid chelating agent and D-gluconic acid-delta-lactone; the liquid material comprises a lithium-based silicate solution, triethanolamine and a fluorocarbon surfactant. According to the method, pH buffer balance and an amino acid complexing zinc source are established through acid lactone hydrolysis, a metastable rheological system is constructed, and the technical problem that high-activity minerals and silicate are mixed and are prone to flash coagulation is solved. After the coating is formed into a film, active ions released and permeated chloride ions are subjected to in-situ reaction to generate layered double hydroxides, so that chemical curing of the chloride ions is realized, and the snow-melting agent corrosion resistance of the concrete guardrail is remarkably improved.

A composition for efficient metal chelation includes a primary chelant. The primary chelant comprises gluconic acid, a gluconate, glucaric acid, a glucarate, one or more derivatives thereof, or a combination thereof. The primary chelant is present in an amount ranging from about 10 wt. % to about 25 wt. % based on total weight of the composition. In addition, the composition includes a secondary chelant. The secondary chelant comprises lactic acid, citric acid, or both lactic acid and citric acid. Further, the composition includes a solvent.