37 results about "Glucaric Acid" patented technology

The invention discloses an inositol oxygenase mutant and application of the inositol oxygenase mutant in preparation of glucaric acid. The protein provided by the invention is as follows: one or more amino acid residues in the 151, 253, 119, 295, 63, 92 and 71 sites of an amino acid sequence of wild inositol oxygenase are replaced to obtain the protein with the same function; the invention provides an enzyme activity enhanced inositol oxygenase mutant which is used for fermentation production of glucaric acid, and the yield of the glucaric acid is improved by 1.5 times.

The invention belongs to the technical field of gene engineering, and discloses recombinant yarrowia lipolytica as well as a construction method and application thereof. The recombinant yarrowia lipolytica PGA1 is obtained by carrying out heterologous expression on an arabidopsis thaliana inositol oxygenase gene and a pseudomonas syringae uronic acid dehydrogenase gene in the yarrowia lipolytica by utilizing a genetic engineering technical means. The recombinant yarrowia lipolytica PGA10 capable of efficiently synthesizing glucaric acid (GA) is finally obtained by further modifying six genes of related metabolic pathways of the recombinant yarrowia lipolytica PGA1. The GA yield of the PGA10 reaches 2233.24 mg / L and is 66.27 times that of PGA1, and the effect is remarkable. The invention not only constructs a GA synthesis method, but also defines the influence of related genes such as opi1 on GA synthesis, and provides reference for the research of heterologous synthesis of GA by microorganisms.

A detergent building composition, comprising (i) one or more sugar oxidation products; and (ii) at least one performance augmenting additive selected from the group consisting of aluminates, silicic acid, sodium silicate, potassium silicate, monosilicates, carbonates, silanes, siloxanes, amino acids, urea, boric acid, borate derivatives, molybdates, titanates, chelating agents, sugar alcohols, carboxylates, acids, and combination thereof. A detergent building composition, comprising (i) glucaric acid, gluconic acid, salts thereof, lactones thereof or combinations thereof; and (ii) at least one performance augmenting additive selected from the group consisting of aluminates, silicic acid, sodium silicate, potassium silicate, monosilicates, carbonates, silanes, siloxanes, amino acids, urea, boric acid, borate derivatives, molybdates, titanates, chelating agents, sugar alcohols, carboxylates, acids, and combination thereof.

The invention belongs to the field of quality analysis and control of traditional Chinese medicines, and discloses a method for determining the content of carpesium abrotanoides components, which comprises the following steps: by taking isochlorogenic acid A as an internal reference substance, calculating the content of chlorogenic acid, caffeic acid, galuteolin, 11 (13)-dehydroaxillary Ivalin, isochlorogenic acid C, 2, 3, 4, 5-tetramethyl-1, 3, 4, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5- According to the method, the content of 10 components of carabrone is rapidly and accurately detected according to the relative correction factors of 2, 3, 4, 5-tetracaffeoyl-D-glucaric acid, 2-deoxy-4-surface-gaillanin, carabrone and dellelactone, and the problem that the detection cost is high due to the fact that many reference substances are needed, are not easy to obtain and are high in price in carabrone content determination is solved.

A method of preparing glucaric acid comprising contacting D-glucose and oxygen with a first catalyst composition comprising a first copper radical oxidase, a single electron oxidizer, and a small molecule activator under conditions suitable for formation of glucodialdose; and contacting glucodialdose and oxygen with a second catalyst composition comprising a second copper radical oxidase, a single electron oxidizer and a small molecule activator under conditions suitable for the formation of a product mixture comprising glucaric acid or salts thereof. A method comprising contacting a sugar with a catalyst composition comprising an oxidoreductase, a single electron oxidizer and a small molecule activator under conditions suitable for the formation of one or more oxidized sugar oxidation products comprising glucaric acid wherein the oxidoreductase comprises at least two copper radical oxidases, at least two mutated copper radical oxidases or combinations thereof.

The invention discloses a monatomic loaded lignin carbon-based photocatalyst as well as a preparation method and application thereof. The preparation method of the photocatalyst comprises the following steps: S1, grinding and blending lignin and melamine, then adding the mixture into water, transferring the obtained solution into a hydrothermal reaction container, reacting at 175-195 DEG C for 4-10 hours, and after the reaction is finished, cooling at room temperature, washing and centrifugally separating to obtain a solid mixture; s2, dispersing the solid mixture in an aqueous solution containing metal salt, stirring at room temperature, and drying after the reaction is finished; and S3, putting the dried solid into a container, putting the container into a pyrolysis reactor, heating to 800-1000 DEG C in an argon atmosphere, pyrolyzing for 2-6 hours, and naturally cooling to obtain the monatomic loaded lignin carbon-based photocatalyst. The monatomic loaded lignin carbon-based photocatalyst provided by the invention can efficiently catalyze glucose to synthesize glucaric acid under mild conditions, and the obtained target product is relatively high in yield and easy to separate, and has a very good industrial application prospect.

The invention discloses a fermentation process for high-yield production of glucaric acid by saccharomyces cerevisiae engineering bacteria. According to the process, a fermentation culture medium is improved and dynamically regulated; the yield of glucaric acid in a 50 L fermentation tank reaches 18 g / L, the production cost is greatly reduced, and industrial production is facilitated.

A polymeric material comprising a base monomer and a multifunctional biobased molecule wherein the multifunctional biobased molecule is a glucose derivative. A polymeric material comprising a base polymer and a polymeric additive comprising glucaric acid, glucodialdose, gluconic acid, erythorbic acid, 2-ketoglucose, lactic acid, adipic acid, sorbitol, glycerol, mannitol, lactones thereof, salts thereof, or combinations thereof wherein the additive when introduced to a base polymer forms a modified polymer wherein the modified polymer has a tensile strength that is increased by from about 10% to about 100% wherein compared to the base polymer.

Provided is a biocatalytic method to synthesize L-guluronic acid and glucaric acid. The technical solution is as follow: 1) Firstly, developed is an enzyme-catalyzed route for synthesizing L-guluronic acid by GOase. 2) Secondly, developed is a biocatalytic route for the synthesis of glucaric acid from L-guluronic acid, catalyzed by uronate dehydrogenase (UDH).

The invention belongs to the technical field of electrocatalytic hydrolysis, and particularly relates to a preparation method and application of high-activity NiCe (OH) n / NF. A noble metal catalyst is not used, so that the use cost is greatly reduced. The positive electrode obtains glucaric acid in an electrooxidation mode, meanwhile, the active center NiOOH can stably exist in an alkaline environment in a circulation mode, and the negative electrode produces hydrogen. And due to the redox cycle between Ni (OH) 2 and NiOOH, the overpotential can be effectively reduced, and the electric energy loss is reduced. And due to the synergistic effect of a small amount of cerium, the system can realize high-efficiency hydrogen production while obtaining the glucaric acid with high additional value. According to the method, glucose is catalyzed by using a circulating system to obtain glucaric acid, meanwhile, the anode glucose yield reaches 70%, and the cathode hydrogen evolution efficiency is close to 100%. Compared with a traditional water electrolysis hydrogen production process, the glucose oxidation assisted low-potential couple circulation coupling hydrogen production method conforms to'green chemistry 'and is lower in cost, and harsh reaction conditions and complex separation steps are avoided.

A method of producing glucaric acid by electrochemical treatment includes (a) providing a reactor including a first electrode-containing region and a second electrode-containing region separated by an electrolyte membrane, adding a glucose mixture to any one region of the reactor, and adding an electrolyte solution to both regions of the reactor. The method also includes (b) producing glucaric acid by oxidizing glucose by applying a voltage between the first electrode and the second electrode in the reactor subjected to step (a). The electrode in the region to which the glucose mixture is added includes a catalyst containing tantalum.

The application discloses a method for separating glucaric acid by using a bimodal pore amphoteric post-crosslinked resin. 2 The bimodal pore amphoteric post-crosslinked resin mainly comprises a precursor resin with a mesoporous structure, and the pore diameter of the resin is cut to form micropores and mesopores through a Friedel-Crafts alkylation reaction. 2 The specific surface area of the bimodal pore amphoteric post-crosslinked resin is 300-800 m 2 / g; the specific surface area of the micropores is 150-500 m / g; and the specific surface area of the mesopores is 100-300 m / g. The bimodal pore amphoteric post-crosslinked resin prepared by the application has a bimodal pore distribution pore structure, a large adsorption capacity for glucaric acid, high selectivity, and is easy to elute after adsorption by using hot water, and can be used as a new type of adsorption medium for separating glucaric acid from a fermentation broth.

The application discloses application of a diphenyl ether compound in preparation of a beta-glucuronidase inhibitor. The diphenyl ether compound is obtained from a fungus Aspergillus versicolor ZJUTE2 through a series of processes such as organic solvent extraction and chromatographic column separation, simple extraction and separation, and has the advantages of simple preparation method, rapidness, high purity of obtained compound and the like; the diphenyl ether compound extracted by the application has good beta-glucuronidase inhibitory activity, and an IC 50 value of 56.59+ / -1.79 muM, is equivalent to a positive drug D-glucaric acid 1,4-lactone, and can be used for treating drug-induced diarrhea caused by irinotecan or non-steroidal anti-inflammatory drugs. In addition, development of structure optimization based on the compound as a lead is of great significance for research and development of a novel drug for treating drug-induced diarrhea.

To provide processes for preparing glucaric acid and / or lactones thereof.SOLUTION: Various processes for preparing aldaric acids, aldonic acids, uronic acids, and / or lactones thereof are described. For example, processes for preparing a C2-C7 aldaric acid and / or lactones thereof by the catalytic oxidation of a C2-C7 aldonic acid and / or lactones thereof and / or a C2-C7 aldose are described.SELECTED DRAWING: None

A method for the production of 2,5-furandicarboxylic acid (FDCA) comprising contacting glucose with a catalyst composition under conditions suitable for the formation of glucarate; acidifying glucarate to form glucaric acid; dehydrating glucaric acid to form FDCA; and recovering FDCA. A method for the production of 2,5-furan dicarboxylic acid dimethyl ester (FDME) comprising contacting glucose with a catalyst composition under conditions suitable for the formation of glycolaldehyde; contacting glycolaldehyde with a transition metal oxidation catalyst under conditions suitable for the formation of glucaric acid; esterifying glucaric acid to form glucaric acid methyl ester; dehydrating the glucaric acid methyl ester to form FDME; and recovering FDME.

A fire mitigating composition comprising (i) a sugar derivative and (ii) a solvent. A fire extinguisher, comprising (i) a sugar derivative, (ii) a solvent and, (iii) a propellent. An article, comprising a wood-containing material having a fire-resistant coating comprising a blend of glucaric acid and gluconic acid. A method of suppressing a fire, comprising applying topically to the surface of a fire or proximate to a fire, a fire mitigating composition comprising a sugar derivative wherein the sugar derivative is selected from the group consisting of an aldaric acid, uronic acid, glucaric acid, gluconic acid, glucuronic acid, glucose oxidation products, gluconic acid oxidation products, disaccharides, oxidized disaccharides, n-keto-acids, C2-C6 diacids, galactonic acid, galactaric acid, glutamic acid, glucodialdose, 2-ketoglucose, glucodiamine, glycoaldehyde, glyoxal, salts thereof, lactones thereof and combinations thereof.

The invention discloses a pharmaceutical composition for treating drug-induced liver injury and application of the pharmaceutical composition, the pharmaceutical composition comprises glucaric acid 1, 4-lactone and N-acetylcysteine, and the mass ratio of the glucaric acid 1, 4-lactone to the N-acetylcysteine is 1: (1-5). The pharmaceutical composition disclosed by the invention can be used for preparing a medicine for treating or preventing the drug-induced liver injury, and the drug-induced liver injury is liver injury caused by excessive acetaminophen or long-term administration. The pharmaceutical composition provides comprehensive liver protection through multi-target cooperation, and has a significant improvement effect on liver injury caused by acetaminophen.

The application belongs to the field of traditional Chinese medicine quality analysis and control, and discloses a determination method of ingredients content of scabiosa comosa, which takes isochlorogenic acid A as an internal reference, calculates the relative correction factors of chlorogenic acid, caffeic acid, jinyingzhi, 11(13)-dehydroxy axillary ivy chrysanthemum, isochlorogenic acid C, 2,3,4,5-tetra caffeoyl-D-glucaric acid, 2-deoxy-4-tablet-Tianrenjuling, scabioside lactone ketone and terlene lactone, quickly and accurately detects the content of 10 kinds of ingredients of scabiosa comosa, and solves the problems of many required control products, difficulty in obtaining and high price in the determination of scabiosa comosa content, and high detection cost.

The invention relates to the technical field of tobacco leaf grade discrimination, in particular to a metabolic marker composition for discriminating the grade of tobacco leaves, a screening method and application. The invention discloses a metabolic marker composition for judging the grade of tobacco leaves. Comprising the following metabolic markers: L-seryl-L-tyrosine, L-tryptophanyl-L-histidine, D-gluconic acid, N-acetylglutamic acid, linoleic acid, alpha-linolenic acid, nicotine, shikimic acid, N-methyl nicotinic acid, O-acetyl-L-carnitine, D-mannose, D-glucaric acid, phosphatidylcholine 36: 1, sucrose, mesaconic acid, fraxin and N-methyltryptamine. By utilizing the 17 metabolic markers, the grade of the tobacco leaves can be effectively distinguished.

The invention belongs to the technical field of medicines, discloses application of D-glucaric acid-1, 4-lactone in preparation of a medicine for relieving intestinal injury caused by sorafenib, and particularly discloses application of D-glucaric acid-1, 4-lactone or pharmaceutically acceptable salt thereof in preparation of a medicine for treating and / or preventing intestinal injury. The invention discloses the application potential of the D-glucaric acid-1, 4-lactone in preparation of medicines for relieving the intestinal injury (especially the intestinal injury caused by molecular targeting medicine sorafenib) for the first time. In the treatment process of sorafenib, patients often need to pause or stop medication due to adverse reactions of gastrointestinal tracts, and the treatment effect of sorafenib is remarkably restricted. Experiments prove that the D-glucaric acid-1, 4-lactone can effectively relieve intestinal injury caused by sorafenib, can relieve mouse intestinal mucosa injury caused by sorafenib, and can relieve intestinal oxidative stress and inflammatory response caused by sorafenib.

Proposed is a method of preparing glucaric acid from glucose through TEMPO oxidation. The method of preparing glucaric acid includes (a) reacting glucose in a solvent through an oxidation reaction using 4-acetamido-TEMPO serving as a nitroxide radical-mediated organocatalyst, potassium bromide serving as a co-catalyst, and potassium hypochlorite serving as an oxidizing agent to prepare glucaric acid. The method can improve the conversion efficiency of glucose to glucaric acid through optimal conditions of the temperature, pH, and input of the oxidizing agent in an oxidation reaction using a TEMPO acid catalyst.

The present invention provides a D-glucaric acid-producing bacterium and a method for producing D-glucaric acid. The present invention is characterized in that D-glucaric acid or a salt thereof is produced from one or more saccharides selected from the group consisting of D-glucose, D-gluconic acid and D-glucuronic acid with catalytic action of a specific alcohol dehydrogenase PQQ-ADH (1) and a specific aldehyde dehydrogenase PQQ-ALDH (2), and that D-glucaric acid or a salt thereof is produced by using a microorganism having the PQQ-ADH (1) and the PQQ-ALDH (2) or a processed product thereof in the presence of the one or more saccharides. The present invention can provide a microorganism having improved productivity of D-glucaric acid to be used for production of D-glucaric acid and a method for efficiently producing D-glucaric acid.

The invention provides a method for biosynthesizing D-glucaric acid by using D-xylose and methanol. At present, most researchers use D-glucose hexose as a substrate to prepare D-glucaric acid. According to the invention, mdh, hps, phi, ino1, suhb, miox and udh genes are over-expressed in escherichia coli, and a new way for biologically synthesizing D-glucaric acid from D-xylose and methanol is constructed; the genes frmRAB, rpiA, pfkA and pfkB are knocked out, so that a carbon source is reasonably controlled; and combining an adaptive laboratory evolution method, fusing a dissolution promoting label and an inositol biosensor to improve the efficiency of a biosynthetic pathway, and finally realizing efficient synthesis of D-glucaric acid.

The application belongs to the technical field of sodium ion battery negative electrode materials, and discloses a corn powder / glucose acid cross-linking derived hard carbon negative electrode material and a preparation method and application thereof; the preparation method is as follows: corn powder and glucose acid are stirred at a ratio of 10:7-20, the modified corn powder after drying is prepared for carbonization at a low temperature of 500-700 DEG C in an inert gas for 1-3 hours, and then high-temperature carbonization is carried out at 1100-1600 DEG C for 1-3 hours to prepare hard carbon. The preparation method of the green and environment-friendly, low-cost and high-cycle-stability sodium ion battery biomass hard carbon negative electrode material has the characteristics of mass production, controllable hard carbon surface morphology and excellent electrochemical performance.

The invention discloses acid-resistant pichia kuchii and an application of the acid-resistant pichia kuchii in glucaric acid synthesis. The method comprises the following steps: by taking pichia kudriavzevii as a chassis, knocking out pgi and / or pfk genes of an EMP pathway, and integrating inositol-phosphate synthase, inositol monophosphate, thermophilic bacteria source inositol oxygenase and uronic acid dehydrogenase genes containing A39P / H99Y / H234K mutation, so as to construct a multi-copy expression system containing four enzymes. And integrating an exogenous gene expression cassette to an EG site by using a CRISPR / Cas9 system, so as to obtain the pichia pastoris engineering bacterium. The glucaric acid yield of the strain in an experiment can reach 3.22 g / L, and the strain has the advantages of being high in acid resistance, free of acid-base neutralization, capable of utilizing cheap substrates and the like. Through combination of metabolic pathway optimization and acid resistance, an efficient and stable microbial platform is provided for industrial production of glucaric acid.

A method of preparing high purity glucaric acid comprising introducing a mixture comprising glucaric acid and one or more sugar oxidation products to a reactive crystallization unit under conditions suitable for formation of an insoluble salt of glucaric acid; and recovering the insoluble salt of glucaric acid. A method of preparing high purity glucaric acid, comprising introducing glucose to a reactor system comprising at least one oxidation reactor and at least one crystallization unit wherein the glucose when disposed in the oxidation reactor is subjected to conditions suitable for the formation of one or more glucose oxidation products and wherein the one or more glucose oxidation products when disposed in the crystallization unit is subjected to conditions suitable for the formation of an insoluble salt of glucaric acid; and recovering the insoluble salt of glucaric acid.

The invention discloses an anti-saccharification and anti-carbonylation composition as well as a preparation method and application thereof, and the composition comprises quinic acid and 2, 3, 4, 5-tetracaffeoyl-D-glucaric acid. According to the invention, quinic acid and 2, 3, 4, 5-tetracaffeoyl-D-glucaric acid are specifically compounded, and the prepared composition can significantly improve the anti-saccharification effect. And a product containing the composition has obvious effects of resisting saccharification, resisting carbonylation, brightening skin color and improving skin darkness, is good in stability and can be applied to cosmetics with corresponding effects.

A method of producing adipic acid using glucose as a starting material, in which adipic acid is finally obtained via an alkyl adipate intermediate from a glucaric acid potassium salt.

A method comprising contacting an amine reactant with a lactone under conditions suitable for the formation of an amide, wherein the lactone comprises glucono-delta lactone (GDL), D-glucaric acid 1,4,6,3 dilactone (GAdL), substituted lactones or combinations thereof. A method of preparing a biobased amide comprising contacting an amine with a sugar-derived lactone under conditions suitable for the formation of an amide.

The application discloses a fusion myo-inositol oxygenase for simultaneously improving soluble expression and catalytic activity and application thereof. The fusion myo-inositol oxygenase is composed of an NusA tag fused to the N terminal of myo-inositol oxygenase (Miox). Compared with wild-type Miox, the soluble expression level of the fusion myo-inositol oxygenase is significantly improved, and the specific enzyme activity reaches 0.98 U / mg, which is 48.5% higher than that of the wild type. The recombinant strain carrying the fusion myo-inositol oxygenase is used for biosynthesis of D-glucaric acid, and the yield of shake flask fermentation reaches 286.5 mg / L, which is 31.9% higher than that of the wild type. The fusion myo-inositol oxygenase provided by the application breaks through the limitation that traditional fusion tags are difficult to simultaneously consider expression and activity, and provides an effective technical scheme for efficient biosynthesis of D-glucaric acid.