13 results about "Glucaric Acid" patented technology

The invention discloses a monatomic loaded lignin carbon-based photocatalyst as well as a preparation method and application thereof. The preparation method of the photocatalyst comprises the following steps: S1, grinding and blending lignin and melamine, then adding the mixture into water, transferring the obtained solution into a hydrothermal reaction container, reacting at 175-195 DEG C for 4-10 hours, and after the reaction is finished, cooling at room temperature, washing and centrifugally separating to obtain a solid mixture; s2, dispersing the solid mixture in an aqueous solution containing metal salt, stirring at room temperature, and drying after the reaction is finished; and S3, putting the dried solid into a container, putting the container into a pyrolysis reactor, heating to 800-1000 DEG C in an argon atmosphere, pyrolyzing for 2-6 hours, and naturally cooling to obtain the monatomic loaded lignin carbon-based photocatalyst. The monatomic loaded lignin carbon-based photocatalyst provided by the invention can efficiently catalyze glucose to synthesize glucaric acid under mild conditions, and the obtained target product is relatively high in yield and easy to separate, and has a very good industrial application prospect.

The application discloses a method for separating glucaric acid by using a bimodal pore amphoteric post-crosslinked resin. 2 The bimodal pore amphoteric post-crosslinked resin mainly comprises a precursor resin with a mesoporous structure, and the pore diameter of the resin is cut to form micropores and mesopores through a Friedel-Crafts alkylation reaction. 2 The specific surface area of the bimodal pore amphoteric post-crosslinked resin is 300-800 m 2 / g; the specific surface area of the micropores is 150-500 m / g; and the specific surface area of the mesopores is 100-300 m / g. The bimodal pore amphoteric post-crosslinked resin prepared by the application has a bimodal pore distribution pore structure, a large adsorption capacity for glucaric acid, high selectivity, and is easy to elute after adsorption by using hot water, and can be used as a new type of adsorption medium for separating glucaric acid from a fermentation broth.

A method for the production of 2,5-furandicarboxylic acid (FDCA) comprising contacting glucose with a catalyst composition under conditions suitable for the formation of glucarate; acidifying glucarate to form glucaric acid; dehydrating glucaric acid to form FDCA; and recovering FDCA. A method for the production of 2,5-furan dicarboxylic acid dimethyl ester (FDME) comprising contacting glucose with a catalyst composition under conditions suitable for the formation of glycolaldehyde; contacting glycolaldehyde with a transition metal oxidation catalyst under conditions suitable for the formation of glucaric acid; esterifying glucaric acid to form glucaric acid methyl ester; dehydrating the glucaric acid methyl ester to form FDME; and recovering FDME.

The application belongs to the field of traditional Chinese medicine quality analysis and control, and discloses a determination method of ingredients content of scabiosa comosa, which takes isochlorogenic acid A as an internal reference, calculates the relative correction factors of chlorogenic acid, caffeic acid, jinyingzhi, 11(13)-dehydroxy axillary ivy chrysanthemum, isochlorogenic acid C, 2,3,4,5-tetra caffeoyl-D-glucaric acid, 2-deoxy-4-tablet-Tianrenjuling, scabioside lactone ketone and terlene lactone, quickly and accurately detects the content of 10 kinds of ingredients of scabiosa comosa, and solves the problems of many required control products, difficulty in obtaining and high price in the determination of scabiosa comosa content, and high detection cost.

The application belongs to the technical field of sodium ion battery negative electrode materials, and discloses a corn powder / glucose acid cross-linking derived hard carbon negative electrode material and a preparation method and application thereof; the preparation method is as follows: corn powder and glucose acid are stirred at a ratio of 10:7-20, the modified corn powder after drying is prepared for carbonization at a low temperature of 500-700 DEG C in an inert gas for 1-3 hours, and then high-temperature carbonization is carried out at 1100-1600 DEG C for 1-3 hours to prepare hard carbon. The preparation method of the green and environment-friendly, low-cost and high-cycle-stability sodium ion battery biomass hard carbon negative electrode material has the characteristics of mass production, controllable hard carbon surface morphology and excellent electrochemical performance.

The invention discloses acid-resistant pichia kuchii and an application of the acid-resistant pichia kuchii in glucaric acid synthesis. The method comprises the following steps: by taking pichia kudriavzevii as a chassis, knocking out pgi and / or pfk genes of an EMP pathway, and integrating inositol-phosphate synthase, inositol monophosphate, thermophilic bacteria source inositol oxygenase and uronic acid dehydrogenase genes containing A39P / H99Y / H234K mutation, so as to construct a multi-copy expression system containing four enzymes. And integrating an exogenous gene expression cassette to an EG site by using a CRISPR / Cas9 system, so as to obtain the pichia pastoris engineering bacterium. The glucaric acid yield of the strain in an experiment can reach 3.22 g / L, and the strain has the advantages of being high in acid resistance, free of acid-base neutralization, capable of utilizing cheap substrates and the like. Through combination of metabolic pathway optimization and acid resistance, an efficient and stable microbial platform is provided for industrial production of glucaric acid.

The invention discloses an anti-saccharification and anti-carbonylation composition as well as a preparation method and application thereof, and the composition comprises quinic acid and 2, 3, 4, 5-tetracaffeoyl-D-glucaric acid. According to the invention, quinic acid and 2, 3, 4, 5-tetracaffeoyl-D-glucaric acid are specifically compounded, and the prepared composition can significantly improve the anti-saccharification effect. And a product containing the composition has obvious effects of resisting saccharification, resisting carbonylation, brightening skin color and improving skin darkness, is good in stability and can be applied to cosmetics with corresponding effects.

The application discloses a fusion myo-inositol oxygenase for simultaneously improving soluble expression and catalytic activity and application thereof. The fusion myo-inositol oxygenase is composed of an NusA tag fused to the N terminal of myo-inositol oxygenase (Miox). Compared with wild-type Miox, the soluble expression level of the fusion myo-inositol oxygenase is significantly improved, and the specific enzyme activity reaches 0.98 U / mg, which is 48.5% higher than that of the wild type. The recombinant strain carrying the fusion myo-inositol oxygenase is used for biosynthesis of D-glucaric acid, and the yield of shake flask fermentation reaches 286.5 mg / L, which is 31.9% higher than that of the wild type. The fusion myo-inositol oxygenase provided by the application breaks through the limitation that traditional fusion tags are difficult to simultaneously consider expression and activity, and provides an effective technical scheme for efficient biosynthesis of D-glucaric acid.

The invention belongs to the technical field of organic compounds, and particularly relates to a high-foam glycosyl amide organosilicon cationic surfactant as well as a preparation method and application thereof. The preparation method of the surfactant comprises the following steps: taking a renewable resource D-gluconic acid delta-lactone as a raw material, carrying out amidation reaction on the raw material and N, N-dimethyl dipropylene triamine to obtain a glucosamide precursor, reacting the precursor with bromododecane, introducing a hydrophobic chain to obtain an intermediate; and finally, carrying out quaternization reaction on the obtained intermediate and 3-chloropropyl methyl dimethoxy silane / 3-chloropropyl trimethoxy silane, so as to synthesize two kinds of glucosamide organosilicon quaternary ammonium salts. No three wastes are discharged in the synthesis process, and the method is suitable for industrial production; the synthesized surfactant has good foamability, foam stability, biocompatibility, no toxicity, no irritation and bactericidal performance, and can play an important role in the fields of medical treatment, self-cleaning, electronics, energy, agriculture, daily chemical industry and the like in the future.

Described herein are processes and enzymes for the enzymatic production of glucaric acid from glucuronic acid, and more specifically the enzymatic production of substituted glucaric acid from substituted glucuronic acid. Advantageously, the process and enzymes described herein make preferential use substituted glucuronic acid obtained from natural sources, such as underutilized hemicellulose stream from wood and agricultural biorefineries, to produce substantially enantiomerically pure substituted D-glucaric acid.

The application discloses a kind of recombinant escherichia coli and its construction method and the method for producing glucaric acid by metabolic engineering, belong to the field of metabolic engineering.The application realizes the production pathway of glucuronic acid by expressing the myo-inositol-1-phosphate synthase gene (Ino1) and myo-inositol oxidase gene (MIOX) derived from plants in escherichia coli, simultaneously expresses the aldehyde acid dehydrogenase gene (Udh) derived from Agrobacterium tumefaciens, and successfully constructs the synthesis pathway of glucaric acid.The recombinant escherichia coli generates glucaric acid 2.53g / L in LB-G (10g / L glucose is added in LB medium) fermentation medium.The method for fermenting and producing glucaric acid by the recombinant escherichia coli proposed in the application has broad development prospect, and lays the foundation for biosynthesis of glucaric acid.

The bioprosthetic valve is treated by a first cross-linking agent and a second cross-linking agent, the first cross-linking agent is L-asparaginic acid, L-glutamic acid, glucaric acid, malic acid or succinic acid, and the second cross-linking agent is chitosan oligosaccharide, L-lysine, arginine or histidine. The invention further provides a preparation method of the bioprosthetic valve, according to the bioprosthetic valve and the preparation method of the bioprosthetic valve, almost non-toxic bio-friendly reagents which are widely applied to food and medicine are adopted, cross-linking is carried out twice in sequence, multi-site stable cross-linking of amino and carboxyl of biological tissue is achieved, the physical performance of materials is improved, and the bioprosthetic valve and the preparation method of the bioprosthetic valve are suitable for large-scale popularization and application. Meanwhile, the calcification problem caused by the toxicity of the cross-linking agent is avoided.

The invention discloses a road guardrail maintenance coating based on an in-situ mineralized chloride ion curing mechanism and a preparation method thereof. Relates to the technical field of concrete protective coatings, and the coating is a two-component system and is composed of an active modified mineral precursor powder material and a functional silicate carrier liquid material. The powder material is prepared from light calcined magnesia, pseudo-boehmite, activated zinc oxide, an amino acid chelating agent and D-gluconic acid-delta-lactone; the liquid material comprises a lithium-based silicate solution, triethanolamine and a fluorocarbon surfactant. According to the method, pH buffer balance and an amino acid complexing zinc source are established through acid lactone hydrolysis, a metastable rheological system is constructed, and the technical problem that high-activity minerals and silicate are mixed and are prone to flash coagulation is solved. After the coating is formed into a film, active ions released and permeated chloride ions are subjected to in-situ reaction to generate layered double hydroxides, so that chemical curing of the chloride ions is realized, and the snow-melting agent corrosion resistance of the concrete guardrail is remarkably improved.