Feed lactic acid bacteria fermentation medium, its preparation method and application

Abstract

The invention provides a feeding lactic acid bacteria fermentation culture medium. The formula comprises 37-43 g / L of glucose, 47-55 g / L of a corn steep liquor dried powder, 42-48 g / L of fish meal, 0-2 g / L of K2HPO4.3H2O, 8-12 g / L of sodium acetate, 2-4 g / L of triammonium citrate, 0-0.5 g / L of MgSO4.7H2O, 0.25-0.5 g / L of MnSO4.H2O and 1-2 ml / L of Twain-80, wherein the pH value is 7.0-7.2, and water is used for preparation. The invention also provides a preparation method and an application of the fermentation culture medium. The cheap and easily available corn steep liquor dried powder and the fish meal are adopted to replace a nitrogen source ingredient in an MRS culture medium, the culture medium components are optimized, and the price of the obtained culture medium is only 40% of that of the MRS culture medium; through optimization of the fermentation initial pH value and the fermentation temperature, and simultaneously through adopting of a way of flowing addition of an NaOH solution to relieve feedback inhibition of lactic acid, the bacteria density is significantly improved compared with that of normal fermentation, the number of viable bacteria in a fermentation liquid is up to 10<10> CFU / ml or more, high density fermentation of feeding lactic acid bacteria is realized, and the specific production rate and the product quality are improved.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a fermentation medium for lactic acid bacteria for feeding, a preparation method and application thereof. Background technique [0002] For a long time, the addition of antibiotics in feed has played a huge role in promoting the development of animal husbandry. However, with the wide application of antibiotics in feed, adverse reactions such as animal intestinal flora imbalance, superinfection and bacterial drug resistance have gradually become prominent. , poses a serious threat to the aquaculture and feed industries. Therefore, it is of great significance to animal husbandry to develop alternatives to antibiotics that have no toxic side effects, no pollution, and no residues. Lactic acid bacteria, as the dominant flora in the digestive tract of pigs, have received extensive attention and research at home and abroad. Studies have shown that there are at least hundreds of ...

AI Technical Summary

Problems solved by technology

[0003] Since lactic acid bacteria cannot synthesize many amino acids, vitamins and related growth factors, their nutritional requirements and culture conditions are complicated. The raw materials of the currently used MRS medium are expensive, especially the nitrogen source components in the medium. More than 80%

[0004] During the growth process of lactic acid bacteria, organic acid metabolites such as lactic acid will be produced, so that the pH value of the medium will continue to drop, thereby inhibiting the growth of lactic acid bacteria, resulting in a decrease in the activity of lactic acid bacteria cells and even the death of the bacteria.

Therefore, adopting the traditional production process, the strain quality cannot be guaranteed, and the production cost is high