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Alcoholic fermentation method for high density yeast cells

A yeast cell and alcohol fermentation technology, which is applied in the field of alcohol fermentation of high-density yeast cells, can solve the problems of high-concentration alcohol fermentation, such as the limitation of wide application, incomplete fermentation, and stagnation of fermentation, so as to improve the germination rate, eliminate inhibition, and prevent acidification effect

Inactive Publication Date: 2013-03-20
吴江永祥酒精制造有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of high-concentration fermentation, high osmotic pressure and high alcohol toxicity will cause yeast death, resulting in slow fermentation, incomplete fermentation, and even stagnant fermentation.
Therefore, the wide application of high-concentration alcoholic fermentation is limited to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for alcoholic fermentation of high-density yeast cells, using the following process steps:

[0028] (1) Add 1000kg cassava flour to a 4000L fermenter, add 2200L tap water according to the solid-liquid mass volume ratio of 1:2.2, adjust the pH to 6.0, and add 10U / g cassava according to the amount of liquefying enzyme, and then raise the temperature to above 80°C Carry out liquefaction, the liquefaction time is 1h.

[0029] After the liquefaction is completed, filter with a plate and frame, wash the filter cake with a small amount of water, and combine the washing water and the filtrate.

[0030] (2) Dividing the liquefied liquid obtained in step (1) into two parts, the first part accounts for 6-15% of the total volume of the liquefied liquid, and the second part accounts for 85-94% of the total volume of the liquefied liquid;

[0031] (3) Cool the first liquefied solution to 60°C, adjust the pH of the liquefied solution to 4.0 with sulfuric acid; add glucoamyla...

Embodiment 2

[0036] A method for alcoholic fermentation of high-density yeast cells, using the following process steps:

[0037] 1. Add 1500kg corn flour to a 5000L fermenter, add 3300L of tap water according to the solid-to-liquid mass volume ratio of 1:2.2, and the rest of the operations are the same as in Example 1.

[0038] 2. Add 1% (w / v) light CaCO to the saccharification solution 3 , and then add 0.1 billion / mL yeast seed liquid to the fermenter, and control the initial fermentation temperature to 30°C;

[0039] 3. After the fermentation is over, the alcohol collected by distillation can be sold as a commodity.

Embodiment 3

[0041] A method for alcoholic fermentation of high-density yeast cells, using the following process steps:

[0042] 1. Add 1000kg corn flour to a 5000L fermenter, add 2000L tap water according to the solid-to-liquid ratio of 1:2.0, and the rest of the operations are the same as in Example 1.

[0043] 2. Add 1% (w / v) light CaCO to the saccharification solution 3 , and then add 0.1 billion / mL yeast seed liquid to the fermenter, and control the initial fermentation temperature to 30°C;

[0044] 3. After the fermentation is over, the alcohol collected by distillation can be sold as a commodity.

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Abstract

The invention discloses an alcoholic fermentation method for high density yeast cells. The alcoholic fermentation method comprises the following steps of: (1) crushing starchy materials, mixing with water, stirring into slurry, adding liquifying enzyme for liquefaction, and separating solid from liquid after liquefaction is completed to obtained liquefied liquid; (2) dividing the liquefied liquid into two parts, and respectively fermenting; (3) fermenting till the sugar concentration in fermentation liquid is lower than 5g / L, and completing fermentation; and (4) mixing the two parts of fermented products, distilling, and collecting alcohol to obtain a finished product. Compared with the prior art, the alcoholic fermentation method for the high density yeast cells has the following advantages that the bud ratio of yeasts is favorably improved, the growth rate of the yeasts is further improved, the high-density fermentation of the yeast cells is realized, the fermentation rate is enhanced, the inhibiting effect of CO2 gas generated in a fermentation process on the yeasts is eliminated, and the fermentation liquid can be prevented from acidifying as CaCO3 has pH buffer function.

Description

technical field [0001] The invention belongs to the technical field of food fermentation industry, and more specifically relates to an alcohol fermentation method of high-density yeast cells. Background technique [0002] The industrialization of alcohol production by fermentation began at the end of the 19th century and played an important role in the national economy. Alcohol is widely used in food, chemical, pharmaceutical, dye, national defense and other industries. Alcohol can not only be used as a beverage in the world, but also a kind of green fuel and a strategic material. As a renewable clean oxygenated fuel, it is expected to gradually replace oil, natural gas and other energy sources. Therefore, it has become a research topic for researchers from various countries. hotspot. Fermentation alcohol has a long history, but the traditional process has low production efficiency and high energy consumption, resulting in high cost. In order to reduce the cost of producin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06
CPCY02E50/17Y02E50/10
Inventor 刘春贞丁永泽白永琦朱建峰周腾云汪李严
Owner 吴江永祥酒精制造有限公司
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