Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Production process for high-density fermentation of sprangium cellulosum and separation coupling of epothilone product

A technology of high-density fermentation and S. cellulosus, applied in the direction of fermentation, microbial-based methods, bacteria, etc., to achieve high production efficiency, convenient operation, and cost-saving effects

Inactive Publication Date: 2011-09-07
QILU UNIV OF TECH
View PDF7 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are only a few domestic patents that have reported the technology of producing epothilone by fermentation of S. cellulosus (ZL02110068.3, ZL02110067.5, ZL200510100338.5), but there is no high-density fermentation of S. cellulosus and epothilone A report on the production process of product separation and coupling

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production process for high-density fermentation of sprangium cellulosum and separation coupling of epothilone product
  • Production process for high-density fermentation of sprangium cellulosum and separation coupling of epothilone product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preparation of S. cellulosus strain: Epothilone production strain S. cellulosus So0157-2, preserved in China Center for Typical Microorganisms Collection, No. M208078. Before use, the strains were activated by culturing in solid activation medium at 28°C for 5 days, and in liquid seed medium at 30°C for 3 days, and the cell volume reached 10 10 When inoculating the fermentation medium, the filling coefficient of the fermentation tank is 70%, the inoculum size is 5% (V / V), and the fermentation period is 7 days.

[0025] The solid activation medium as described in 1 is: 0.5% potato starch, 1% soybean peptone, 1% glucose, 0.5% yeast extract, MgSO 4 0.5%, CaCl 2 0.5%; TE 1.0ml / L; pH7.0. Add 0.8% agar and sterilize at 121°C for 20 minutes before use.

[0026] The liquid seed medium as described in 1 is: 0.5% potato starch, 1% soybean peptone, 1% glucose, 0.5% yeast extract, MgSO 4 0.5%, CaCl 2 0.5%; TE 1.0ml / L; pH7.0. Sterilize at 121°C for 20 minutes before use.

...

Embodiment 2

[0034] 1. Preparation of S. cellulosus strain: Epothilone production strain S. cellulosus So0157-2, preserved in China Center for Typical Microorganisms Collection, No. M208078. Before use, the strains were activated by culturing solid activation medium at 30°C for 6 days, and culturing liquid seed medium at 31°C for 4 days, and the cell volume reached 10 10 When inoculating the fermentation medium, the filling coefficient of the fermentation tank is 70%, the inoculum size is 10% (V / V), and the fermentation period is 8 days.

[0035] The solid-state activation medium as described in 1 is: 1% potato starch, 1.5% soybean peptone, 1.5% glucose, 0.8% yeast extract, MgSO 4 0.7%, CaCl 2 0.7%; TE 1.5ml / L; pH7.5. Add 1% agar and sterilize at 121°C for 20 minutes before use.

[0036] The liquid seed medium as described in 1 is: 2% potato starch, 1.5% soybean peptone, 1.5% glucose, 0.8% yeast extract, MgSO 4 0.7%, CaCl 2 0.7%; TE 1.5ml / L; pH7.5. Sterilize at 121°C for 20 minutes be...

Embodiment 3

[0044] 1. Preparation of S. cellulosus strain: Epothilone production strain S. cellulosus So0157-2, preserved in China Center for Typical Microorganisms Collection, No. M208078. Before use, the strains were activated by culturing in solid activation medium at 32°C for 7 days, and in liquid seed medium for 5 days at 32°C, and the cell volume reached 10 10 When inoculating the fermentation medium, the filling coefficient of the fermentation tank is 70%, the inoculum size is 5% (V / V), and the fermentation period is 9 days.

[0045] The solid activation medium as described in 1 is: 3% potato starch, 2% soybean peptone, 2% glucose, 1% yeast extract, MgSO 4 1%, CaCl 2 1%; TE 2.0ml / L; pH8.0. Add 1.5% agar, and sterilize at 121°C for 20 minutes before use.

[0046] The liquid seed medium as described in 1 is: 3% potato starch, 2% soybean peptone, 2% glucose, 1% yeast extract, MgSO 4 1%, CaCl 2 1%; TE 2.0ml / L; pH8.0. Sterilized at 121°C for 20 minutes before use.

[0047] The fer...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a production process for high-density fermentation of sprangium cellulosum and separation coupling of an epothilone product. In the invention, the high-density fermentation of the sprangium cellulosum is realized by feeding carbon and nitrogen source substances and precursors; and at the same time, a polymeric adsorbent which can be repeatedly used can be used for separating the epothilone product and the high-density fermentation and product separation coupling are realized, thus feedback inhibition of the epothilone on the sprangium cellulosum is removed and output of the epothilone is increased. The invention has the technical characteristics of cost conservation, simple process, convenience in operation and high production efficiency.

Description

technical field [0001] The invention relates to a production process of high-density fermentation of Sonocystis cellulosus and the separation and coupling of epothilone products, belonging to the technical field of fermentation engineering. Background technique [0002] In 1993, G. et al. reported that they isolated Epothilone A and B ( figure 1 ). In 1995, D.M.Bollag of Merck Research Institute found that a fermented extract of S. cellulosus SMP 44 had the activity of inhibiting tubulin depolymerization and disrupting mitosis during large-scale screening of microtubule inhibitors. The active components are Epothilone A and B, which have the same mechanism of action as paclitaxel to inhibit tumor cell growth, and have more advantages than paclitaxel. In 2007, Ixapirone, a derivative of epothilones B, was approved for marketing by the US FDA. At present, six epothilones have entered clinical research, and epothilones are emerging in the field of anticancer drugs today. ,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/18C07D493/04C12R1/01
Inventor 刘新利赵林陆震高海燕顾文雪李亚伟
Owner QILU UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com