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31results about How to "No effect on function" patented technology

Method for secretory expression of super-folded green fluorescent protein mediated heterologous protein in escherichia coli

The invention provides a method for secretory expression of super-folded green fluorescent protein mediated heterologous protein in escherichia coli. The method includes steps: 1) constructing a secretory expression carrier pET23a / sfGFP-GFP taking sfGFP (super-folded green fluorescent protein) as a secretory tag; 2) constructing a heterologous protein gene recombinant expression carrier; 3) respectively converting and fusing the expression carriers to escherichia coli competent cell Rosetta Blue to obtain a recombinant strain; 4) expressing, culturing and performing functional verification; 5) performing high-density fermentation of the recombinant strain. According to secretion characteristics of the super-folded green fluorescent protein, extracellular secretory expression of the heterologous protein in escherichia coli in a fusion protein form is realized without mediating through signal peptides, an operation process is simplified, high autocrine performance is realized, functional influences of tag protein on target protein are avoided, expression conditions can be quickly optimized, target protein yield is increased, and the method is suitable for large-scale production. In addition, an application field of sfGFP is expanded, and a novel method is provided for extracellular secretory expression of the heterologous protein in escherichia coli.
Owner:HUBEI UNIV

Electronic remote control lock head with emergency unlocking function

ActiveCN104533172ARealize emergency manual openingTurn on smoothlyNon-mechanical controlsCylinder locksRemote controlElectric machinery
The invention discloses an electronic remote control lock head with an emergency unlocking function, and belongs to the field of electronic locks. The electronic remote control lock head comprises a lock shell, a motor, a dial wheel, a dial wheel driving piece, an unlocking rod, an unlocking pinball, an unlocking matching spring and a lock cylinder. The motor, the dial wheel driving piece and the unlocking rod are sequentially mounted in the lock shell, a pinball containing groove is formed in the end, matched with the dial wheel driving piece, of the unlocking rod, and a stopping block is arranged in the pinball containing groove. One end of the dial wheel driving piece is connected with the motor, and the other end of the dial wheel driving piece is provided with a boss which is located in the pinball containing groove and is free of rotating interference with the stopping block. The dial wheel is fixed on the dial wheel driving piece, the unlocking pinball and the unlocking matching spring are sequentially arranged on the lower portion of the pinball containing groove, the lower end of the unlocking matching spring is matched with the lock cylinder, and the lock cylinder rotates to enable the unlocking pinball to enter or exit from the pinball containing groove. The electronic remote control lock head can be unlocked by an emergency key in emergency circumstances, and the trouble and loss of overall dismounting of the electronic lock are avoided; the concealment of an emergency key hole is good, and the safety is high.
Owner:陈京杰

Refractive index matching method adaptive to microfluidic-X-ray plate imaging and application thereof

The invention discloses a refractive index matching method adaptive to microfluidic-X-ray plate imaging and an application thereof. The method comprises the following step: an imaging environment of biological sample X-ray plate imaging is adjusted by using iodixanol, so that the refractive indexes of adjacent media in the imaging environment are matched, wherein the adjacent media are respectively a microfluidic chip used for fixing a biological sample and imaging liquid containing the biological sample, or the adjacent media are respectively oil and water on water-in-oil droplets containingthe biological sample and formed by the micro-fluidic chip. An iodixanol solution is introduced, and the refractive index of the imaging environment is adjusted by changing the concentration of iodixanol. The adjustment range is wide, and the refractive indexes of adjacent media in the imaging environment can be matched. The scattering phenomenon of illumination light on an oil-phase and water-phase interface and a solid-liquid interface of a PDMS-solution is eliminated. The problems of scattering and aberration of imaging are solved. Meanwhile, iodixanol has biocompatibility, real-time imaging can be achieved, and the imaging quality is improved.
Owner:HUAZHONG UNIV OF SCI & TECH

Traditional Chinese medicine medical pillow used for treating cervical spondylosis

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine medical pillow which is exclusively used for treating cervical spondylosis. The traditional Chinese medicine medical pillow comprises an external pillowslip with air permeability and traditional Chinese medicines filled into the pillowslip, wherein the traditional Chinese medicines comprise the following raw materials according to parts by weight: 10-30 parts of safflower, 10-30 parts of myrrh, 10-30 parts of mastic, 20-40 parts of lycopodium clavatum, 20-40 parts of garden balsam stem, 20-40 parts of radix sileris, 30-40 parts of pubescent angelica root, 30-40 parts of radix puerariae, 5-20 parts of tortoise plastron, 20-40 parts of salvia chinensis, 20-40 parts of borneol, 10-30 parts of red peony root, 10-40 parts of chrysanthemum and 10-30 parts of peppermint. The invention has obvious curative effect to the cervical spondylosis, and the cure rate can reach 95%. As the pillowslip of the traditional Chinese medicine medical pillow has reasonable size design and is matched with the size of the neck of a person, the traditional Chinese medicine medical pillow can also be used as a daily health-care pillow. The invention has stronger and very extensive market application prospect.
Owner:姜明洲

Active clamping forward-and-flyback circuit capable of starting or stopping synchronous rectification

The invention relates to an active clamping forward-and-flyback circuit capable of starting or stopping synchronous rectification. According to the active clamping forward-and-flyback circuit capable of starting or stopping the synchronous rectification, an acquisition and isolating circuit and a comparison and driving circuit are added between a primary edge and a secondary edge, the output of a main tube driving circuit of a main circuit is connected with the comparison and driving circuit through the acquisition and isolating circuit, and the comparison and driving circuit is connected with a synchronous rectification tube control end on the secondary edge. According to the active clamping forward-and-flyback circuit capable of starting or stopping the synchronous rectification, the driving stopping of a synchronous rectification tube is achieved on the secondary edge, and the synchronous stopping of the synchronous rectification of the secondary edge is guaranteed when a main tube on the primary edge is switched off. The active clamping forward-and-flyback circuit capable of starting or stopping the synchronous rectification has the advantages that the problem that the main tube is impacted by resonance voltage when energy of the secondary edge of an original active clamping circuit is recovered is solved, the principle of the circuit is simple and reasonable, and other functions and indexes of the circuit will not be affected.
Owner:嘉善中正电子科技有限公司

Traditional Chinese medicine preparation used for treating fungal dermatopathy

The invention relates to the technical field of traditional Chinese medicines for treating dermatopathy, in particular to a traditional Chinese medicine preparation which is exclusively used for treating fungal dermatopathy. The traditional Chinese medicine preparation comprises the following raw materials in parts by weight: 2-10 of safflower, 2-15 of chrysanthemum, 2-10 of seed of jog's tears, 5-15 of red peony root, 5-15 of radix salviae miltiorrhizae, 2-10 of spatholobus stem, 2-10 of angelica, 2-20 of oriental wormwood, 5-15 of dandelion, 2-10 of radix sileris, 5-10 of dried ginger, 2-15 of coptis root, 5-10 of zanthoxylum and 5-10 of peppermint. The invention has wide source of the used medicinal materials and low and reasonable price and has the effects of promoting the blood circulation, relieving the stasis of blood, decreasing swelling, relieving pain, destroying parasites, relieving itching and lessening the virulence of any pathogenic organism. The medicine has obvious curative effect, no side effect, safe and reliable use and short treatment period, and the cure rate can reach 80%. The invention can enable symptoms to achieve the aim of recovery in the shortest time, can relieve the ailment of extensive patients in a fastest time and has stronger market application prospect.
Owner:戚寰

Externally-applied ostealgia-eliminating paste used for treating cervical spondylosis and lumbar vertebra diseases

The invention relates to the technical field of traditional Chinese medicine pastes, in particular to a special externally-applied ostealgia-eliminating paste for treating cervical spondylosis and lumbar vertebra diseases. The special externally-applied ostealgia-eliminating paste comprises the following raw materials according to parts by weight: 10-30 parts of safflower, 10-30 parts of myrrh, 10-30 parts of mastic, 20-40 parts of lycopodium clavatum, 20-40 parts of garden balsam stem, 20-40 parts of radix sileris, 30-40 parts of pubescent angelica root, 30-40 parts of radix puerariae, 5-20 parts of tortoise plastron, 20-40 parts of salvia chinensis and 20-40 parts of borneol. The externally-applied paste prepared by the invention has wide source of medicinal materials, reasonable price, no side effect, safe and reliable use and short treating period, has the functions of dispersing pathogenic wind, smoothing collaterals, promoting blood circulation, relieving the stasis of blood, nourishing the liver and the kidney and alleviating pain and has obvious curative effect to the cervical spondylosis and the lumbar vertebra diseases, and the total effective treating rate reaches 95%. The invention can achieve the aim of recovering symptoms in the shortest time and has stronger market application prospect.
Owner:姜明洲

Traditional Chinese medicine medicated wine used for treating cervical spondylosis

The invention relates to the technical field of traditional Chinese medicine medicated wine, in particular to traditional Chinese medicine medicated wine which is exclusively used for treating cervical spondylosis. The traditional Chinese medicine medicated wine is formed by infusing traditional Chinese medicines in grain wine, and the ratio of the grain wine to the traditional Chinese medicines is 90:10 according to parts by weight; the traditional Chinese medicines comprise the following raw materials according to parts by weight: 10-30 parts of tangerine peel, 30-40 parts of angelica, 30-40 parts of radix puerariae, 5-20 parts of white peony root, 5-20 parts of spatholobus stem, 5-20 parts of radix salviae miltiorrhizae, 20-40 parts of licorice, 20-40 parts of pawpaw, 10-40 parts of chrysanthemum and 10-30 parts of rock candy. The invention has extensive source of the used medicinal materials and reasonable price, has the functions of dispersing pathogenic wind, smoothing collaterals, promoting blood circulation, relieving the stasis of blood, nourishing the liver and the kidney and alleviating pain and has obvious curative effect to the cervical spondylosis. Traditional Chinese medicine medicated wine used for treating cervical spondylosis has good mouth feel and can be taken safely and reliably and can also be used as daily health-care wine for taking. The invention has stronger and very extensive market application prospect and obvious social benefit and economic benefit.
Owner:姜明洲

High-strength anchor net mortar pouring, reinforcing, leaking stoppage and repairing method for underground structure leakage

PendingCN112681399AOvercoming the defect of repeated leakageIncreased durabilityArtificial islandsProtective foundationFiberArchitectural engineering
The invention discloses a high-strength anchor net mortar pouring, reinforcing, leaking stoppage and repairing method for underground structure leakage. According to the high-strength anchor net mortar pouring, reinforcing, leaking stoppage and repairing method for underground structure leakage, on the basis that roughening is carried out on a leakage surface, blocking is carried out on large-strand water flow through drainage, grouting and the like; short anchor bolts are arranged in a structure leakage area according to a certain row spacing and depth; a steel wire mesh is arranged, and fixing the steel wire mesh and the anchor bolts together is carried out; compact mortar with certain slump is prepared, and a proper amount of anti-crack fiber is added; and mortar materials are distributed in the leakage area, vibration pouring is carried out through template fixing, gas in the mortar is exhausted, and the compactness of the mortar is improved. Thus, after the mortar is solidified to form strength, a high-strength compact flexible mortar waterproof anchoring layer is formed on the structure leakage inner surface, and a seepage channel is permanently blocked; so that the whole leakage treatment time is short, the manufacturing cost is low, and the extremely important application and popularization value is achieved.
Owner:陆建梅

A method for the secretion and expression of heterologous proteins mediated by superfolded green fluorescent protein in Escherichia coli

The invention provides a method for secretory expression of super-folded green fluorescent protein mediated heterologous protein in escherichia coli. The method includes steps: 1) constructing a secretory expression carrier pET23a / sfGFP-GFP taking sfGFP (super-folded green fluorescent protein) as a secretory tag; 2) constructing a heterologous protein gene recombinant expression carrier; 3) respectively converting and fusing the expression carriers to escherichia coli competent cell Rosetta Blue to obtain a recombinant strain; 4) expressing, culturing and performing functional verification; 5) performing high-density fermentation of the recombinant strain. According to secretion characteristics of the super-folded green fluorescent protein, extracellular secretory expression of the heterologous protein in escherichia coli in a fusion protein form is realized without mediating through signal peptides, an operation process is simplified, high autocrine performance is realized, functional influences of tag protein on target protein are avoided, expression conditions can be quickly optimized, target protein yield is increased, and the method is suitable for large-scale production. In addition, an application field of sfGFP is expanded, and a novel method is provided for extracellular secretory expression of the heterologous protein in escherichia coli.
Owner:HUBEI UNIV

An electronic remote control lock head with emergency opening function

The invention discloses an electronic remote control lock head with an emergency unlocking function, and belongs to the field of electronic locks. The electronic remote control lock head comprises a lock shell, a motor, a dial wheel, a dial wheel driving piece, an unlocking rod, an unlocking pinball, an unlocking matching spring and a lock cylinder. The motor, the dial wheel driving piece and the unlocking rod are sequentially mounted in the lock shell, a pinball containing groove is formed in the end, matched with the dial wheel driving piece, of the unlocking rod, and a stopping block is arranged in the pinball containing groove. One end of the dial wheel driving piece is connected with the motor, and the other end of the dial wheel driving piece is provided with a boss which is located in the pinball containing groove and is free of rotating interference with the stopping block. The dial wheel is fixed on the dial wheel driving piece, the unlocking pinball and the unlocking matching spring are sequentially arranged on the lower portion of the pinball containing groove, the lower end of the unlocking matching spring is matched with the lock cylinder, and the lock cylinder rotates to enable the unlocking pinball to enter or exit from the pinball containing groove. The electronic remote control lock head can be unlocked by an emergency key in emergency circumstances, and the trouble and loss of overall dismounting of the electronic lock are avoided; the concealment of an emergency key hole is good, and the safety is high.
Owner:陈京杰

A Refractive Index Matching Method Adapted to Microfluidic-Light Sheet Imaging and Its Application

The invention discloses a refractive index matching method adapted to microfluidic-light-sheet imaging and its application, including: using iodixanol to adjust the imaging environment of biological sample light-sheet imaging, so that the adjacent medium in the imaging environment Refractive index matching between them; wherein, the adjacent media are respectively the microfluidic chip used to fix the biological sample and the imaging fluid containing the biological sample, or the adjacent media are respectively the biological fluid contained in the microfluidic chip. Oil and water on a water-in-oil droplet of a sample. The present invention introduces iodixanol solution, and adjusts the refractive index of the imaging environment by changing the concentration of iodixanol. The adjustment range is wide, so that the refractive index of the adjacent medium in the imaging environment is matched, and the illumination light is eliminated between the oil phase and the water. The phase interface and the solid-liquid interface scattering phenomenon of PDMS-solution solve the problem of scattering and aberration in imaging. At the same time, iodixanol is biocompatible and can be used for real-time imaging, which effectively improves the imaging quality.
Owner:HUAZHONG UNIV OF SCI & TECH
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