31results about How to "No effect on function" patented technology

The invention provides a method for secretory expression of super-folded green fluorescent protein mediated heterologous protein in escherichia coli. The method includes steps: 1) constructing a secretory expression carrier pET23a / sfGFP-GFP taking sfGFP (super-folded green fluorescent protein) as a secretory tag; 2) constructing a heterologous protein gene recombinant expression carrier; 3) respectively converting and fusing the expression carriers to escherichia coli competent cell Rosetta Blue to obtain a recombinant strain; 4) expressing, culturing and performing functional verification; 5) performing high-density fermentation of the recombinant strain. According to secretion characteristics of the super-folded green fluorescent protein, extracellular secretory expression of the heterologous protein in escherichia coli in a fusion protein form is realized without mediating through signal peptides, an operation process is simplified, high autocrine performance is realized, functional influences of tag protein on target protein are avoided, expression conditions can be quickly optimized, target protein yield is increased, and the method is suitable for large-scale production. In addition, an application field of sfGFP is expanded, and a novel method is provided for extracellular secretory expression of the heterologous protein in escherichia coli.

The invention relates to a temperature sensing milk bottle, which is made of plastic or silica gel suitable for the milk bottle; a temperature sensing and color developing area is arranged on the side surface of a bottle body; the single lamellar bottle body is formed by integrating the temperature sensing and color developing area with the peripheral area; and the temperature sensing and color developing area is modulated in a manner of presenting a first color when the temperature is in a first temperature range and presenting a second color when the temperature rises to a second temperature range. The temperature sensing milk bottle has the advantages that on the basis that the structure, a material and the process of the existing milk bottle are maintained as much as possible, the function of sensing temperature changes in the specific area on the bottle body through color changes is provided.

The invention discloses a closed-type mechanical code lock and relates to the field of dial-type combination mechanical code locks. The six dials of the code lock are correspondingly arranged on separate dial shafts; the unlocking codes of the dials can be replaced; the operation is simple, accurate and reliable; when insertion plates in a lock body are inserted into all openings of the dials at the same time, the lock can only be opened; and the lock body is internally provided with a sealing plate. During combination, a knob shaft in the outer panel of the lock can pass through the corresponding through hole of the sealing plate to complete the number checking operation, but the dials and the insertion plates have no contact; when the lock is opened, the sealing plate first closes a checking channel for checking numbers and re-drives to unlock, that is, when the dials make contact with the insertion plates, the checking channel is closed, so the position of the dial opening cannot bejudged at all, that is, the technical opening cannot be performed; when a handle is strongly reversed, the handle can be idling with overload protection; even if the outer panel of the lock is removed or destroyed, the lock cannot be technically opened; and the exposed camshaft can still idle.

The invention discloses an electronic remote control lock head with an emergency unlocking function, and belongs to the field of electronic locks. The electronic remote control lock head comprises a lock shell, a motor, a dial wheel, a dial wheel driving piece, an unlocking rod, an unlocking pinball, an unlocking matching spring and a lock cylinder. The motor, the dial wheel driving piece and the unlocking rod are sequentially mounted in the lock shell, a pinball containing groove is formed in the end, matched with the dial wheel driving piece, of the unlocking rod, and a stopping block is arranged in the pinball containing groove. One end of the dial wheel driving piece is connected with the motor, and the other end of the dial wheel driving piece is provided with a boss which is located in the pinball containing groove and is free of rotating interference with the stopping block. The dial wheel is fixed on the dial wheel driving piece, the unlocking pinball and the unlocking matching spring are sequentially arranged on the lower portion of the pinball containing groove, the lower end of the unlocking matching spring is matched with the lock cylinder, and the lock cylinder rotates to enable the unlocking pinball to enter or exit from the pinball containing groove. The electronic remote control lock head can be unlocked by an emergency key in emergency circumstances, and the trouble and loss of overall dismounting of the electronic lock are avoided; the concealment of an emergency key hole is good, and the safety is high.

The invention discloses a refractive index matching method adaptive to microfluidic-X-ray plate imaging and an application thereof. The method comprises the following step: an imaging environment of biological sample X-ray plate imaging is adjusted by using iodixanol, so that the refractive indexes of adjacent media in the imaging environment are matched, wherein the adjacent media are respectively a microfluidic chip used for fixing a biological sample and imaging liquid containing the biological sample, or the adjacent media are respectively oil and water on water-in-oil droplets containingthe biological sample and formed by the micro-fluidic chip. An iodixanol solution is introduced, and the refractive index of the imaging environment is adjusted by changing the concentration of iodixanol. The adjustment range is wide, and the refractive indexes of adjacent media in the imaging environment can be matched. The scattering phenomenon of illumination light on an oil-phase and water-phase interface and a solid-liquid interface of a PDMS-solution is eliminated. The problems of scattering and aberration of imaging are solved. Meanwhile, iodixanol has biocompatibility, real-time imaging can be achieved, and the imaging quality is improved.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine medical pillow which is exclusively used for treating cervical spondylosis. The traditional Chinese medicine medical pillow comprises an external pillowslip with air permeability and traditional Chinese medicines filled into the pillowslip, wherein the traditional Chinese medicines comprise the following raw materials according to parts by weight: 10-30 parts of safflower, 10-30 parts of myrrh, 10-30 parts of mastic, 20-40 parts of lycopodium clavatum, 20-40 parts of garden balsam stem, 20-40 parts of radix sileris, 30-40 parts of pubescent angelica root, 30-40 parts of radix puerariae, 5-20 parts of tortoise plastron, 20-40 parts of salvia chinensis, 20-40 parts of borneol, 10-30 parts of red peony root, 10-40 parts of chrysanthemum and 10-30 parts of peppermint. The invention has obvious curative effect to the cervical spondylosis, and the cure rate can reach 95%. As the pillowslip of the traditional Chinese medicine medical pillow has reasonable size design and is matched with the size of the neck of a person, the traditional Chinese medicine medical pillow can also be used as a daily health-care pillow. The invention has stronger and very extensive market application prospect.

The invention relates to an active clamping forward-and-flyback circuit capable of starting or stopping synchronous rectification. According to the active clamping forward-and-flyback circuit capable of starting or stopping the synchronous rectification, an acquisition and isolating circuit and a comparison and driving circuit are added between a primary edge and a secondary edge, the output of a main tube driving circuit of a main circuit is connected with the comparison and driving circuit through the acquisition and isolating circuit, and the comparison and driving circuit is connected with a synchronous rectification tube control end on the secondary edge. According to the active clamping forward-and-flyback circuit capable of starting or stopping the synchronous rectification, the driving stopping of a synchronous rectification tube is achieved on the secondary edge, and the synchronous stopping of the synchronous rectification of the secondary edge is guaranteed when a main tube on the primary edge is switched off. The active clamping forward-and-flyback circuit capable of starting or stopping the synchronous rectification has the advantages that the problem that the main tube is impacted by resonance voltage when energy of the secondary edge of an original active clamping circuit is recovered is solved, the principle of the circuit is simple and reasonable, and other functions and indexes of the circuit will not be affected.

A Chinese medicine for preventing and treating the viral infectious diseases, such as plague and SARS, is prepared from 7 Chinese-medicinal materials including astragalus root, honeysuckle flower, isatis leaf, liquorice root, etc. Its preparing process is also disclosed. Its advantages are sure curative effect, and no toxic by-effect.

The present method of nose-lifting material includes the following steps: soaking the tooth separated from human body in hydrochloride acid whose concentration is 5%-20% to make demineralization, then cutting the tooth body into leaflet from or firstly cutting the tooth body into leaflet form, then soaking it in hydrochloric acid to make demineralization; the connecting the demineralized leaflet tooth tissue into hump nose prosthesis by means of titanium wire; finally regulating and grinding the hump nose prosthesis into individual prosthesis according to the facial model. Said invention uses human tooth as main raw material, the decalcified tooth body can be arbitrarily moulded according to the requirment, and possesses good tissue compatibility, and possesses good affinity with peripheral tissue, after it is transplanted, it is not easy to be absorbed.

The invention relates to the technical field of traditional Chinese medicines for treating dermatopathy, in particular to a traditional Chinese medicine preparation which is exclusively used for treating fungal dermatopathy. The traditional Chinese medicine preparation comprises the following raw materials in parts by weight: 2-10 of safflower, 2-15 of chrysanthemum, 2-10 of seed of jog's tears, 5-15 of red peony root, 5-15 of radix salviae miltiorrhizae, 2-10 of spatholobus stem, 2-10 of angelica, 2-20 of oriental wormwood, 5-15 of dandelion, 2-10 of radix sileris, 5-10 of dried ginger, 2-15 of coptis root, 5-10 of zanthoxylum and 5-10 of peppermint. The invention has wide source of the used medicinal materials and low and reasonable price and has the effects of promoting the blood circulation, relieving the stasis of blood, decreasing swelling, relieving pain, destroying parasites, relieving itching and lessening the virulence of any pathogenic organism. The medicine has obvious curative effect, no side effect, safe and reliable use and short treatment period, and the cure rate can reach 80%. The invention can enable symptoms to achieve the aim of recovery in the shortest time, can relieve the ailment of extensive patients in a fastest time and has stronger market application prospect.

The invention relates to the technical field of traditional Chinese medicine pastes, in particular to a special externally-applied ostealgia-eliminating paste for treating cervical spondylosis and lumbar vertebra diseases. The special externally-applied ostealgia-eliminating paste comprises the following raw materials according to parts by weight: 10-30 parts of safflower, 10-30 parts of myrrh, 10-30 parts of mastic, 20-40 parts of lycopodium clavatum, 20-40 parts of garden balsam stem, 20-40 parts of radix sileris, 30-40 parts of pubescent angelica root, 30-40 parts of radix puerariae, 5-20 parts of tortoise plastron, 20-40 parts of salvia chinensis and 20-40 parts of borneol. The externally-applied paste prepared by the invention has wide source of medicinal materials, reasonable price, no side effect, safe and reliable use and short treating period, has the functions of dispersing pathogenic wind, smoothing collaterals, promoting blood circulation, relieving the stasis of blood, nourishing the liver and the kidney and alleviating pain and has obvious curative effect to the cervical spondylosis and the lumbar vertebra diseases, and the total effective treating rate reaches 95%. The invention can achieve the aim of recovering symptoms in the shortest time and has stronger market application prospect.

The invention relates to the technical field of traditional Chinese medicine medicated wine, in particular to traditional Chinese medicine medicated wine which is exclusively used for treating cervical spondylosis. The traditional Chinese medicine medicated wine is formed by infusing traditional Chinese medicines in grain wine, and the ratio of the grain wine to the traditional Chinese medicines is 90:10 according to parts by weight; the traditional Chinese medicines comprise the following raw materials according to parts by weight: 10-30 parts of tangerine peel, 30-40 parts of angelica, 30-40 parts of radix puerariae, 5-20 parts of white peony root, 5-20 parts of spatholobus stem, 5-20 parts of radix salviae miltiorrhizae, 20-40 parts of licorice, 20-40 parts of pawpaw, 10-40 parts of chrysanthemum and 10-30 parts of rock candy. The invention has extensive source of the used medicinal materials and reasonable price, has the functions of dispersing pathogenic wind, smoothing collaterals, promoting blood circulation, relieving the stasis of blood, nourishing the liver and the kidney and alleviating pain and has obvious curative effect to the cervical spondylosis. Traditional Chinese medicine medicated wine used for treating cervical spondylosis has good mouth feel and can be taken safely and reliably and can also be used as daily health-care wine for taking. The invention has stronger and very extensive market application prospect and obvious social benefit and economic benefit.

The invention discloses an oscillatory flow filtering method for an electronic meter at a zero flow point. The method comprises the following steps: carrying out the collection of the flow of the meter at an initial sampling time interval t1, and obtaining the real-time flow Qs; judging whether the flow Qs is less than the starting flow of the meter or not, if yes, determining that the current instantaneous flow Q of the meter is equal to 0, and if not, determining that the current instantaneous flow Q of the meter is equal to Qs; recording the current instantaneous flow Q of the meter and thedirections of flow Q1, Q2,..., QN obtained by N times of sampling before Q, wherein N is an integer greater than or equal to 2; and judging whether the absolute value of the current instantaneous flow Q is less than a preset flow threshold value or not, and if the absolute value of the current instantaneous flow Q is less than the preset flow threshold value, performing zero-flow oscillation flowfiltering. The oscillation flow near the zero flow can be effectively filtered, the economic loss of the user caused by the zero flow oscillation flow is reduced, and the normal flow and the functions of the meter are not influenced.

The invention discloses a high-strength anchor net mortar pouring, reinforcing, leaking stoppage and repairing method for underground structure leakage. According to the high-strength anchor net mortar pouring, reinforcing, leaking stoppage and repairing method for underground structure leakage, on the basis that roughening is carried out on a leakage surface, blocking is carried out on large-strand water flow through drainage, grouting and the like; short anchor bolts are arranged in a structure leakage area according to a certain row spacing and depth; a steel wire mesh is arranged, and fixing the steel wire mesh and the anchor bolts together is carried out; compact mortar with certain slump is prepared, and a proper amount of anti-crack fiber is added; and mortar materials are distributed in the leakage area, vibration pouring is carried out through template fixing, gas in the mortar is exhausted, and the compactness of the mortar is improved. Thus, after the mortar is solidified to form strength, a high-strength compact flexible mortar waterproof anchoring layer is formed on the structure leakage inner surface, and a seepage channel is permanently blocked; so that the whole leakage treatment time is short, the manufacturing cost is low, and the extremely important application and popularization value is achieved.

The invention discloses an electronic remote control lock head with an emergency unlocking function, and belongs to the field of electronic locks. The electronic remote control lock head comprises a lock shell, a motor, a dial wheel, a dial wheel driving piece, an unlocking rod, an unlocking pinball, an unlocking matching spring and a lock cylinder. The motor, the dial wheel driving piece and the unlocking rod are sequentially mounted in the lock shell, a pinball containing groove is formed in the end, matched with the dial wheel driving piece, of the unlocking rod, and a stopping block is arranged in the pinball containing groove. One end of the dial wheel driving piece is connected with the motor, and the other end of the dial wheel driving piece is provided with a boss which is located in the pinball containing groove and is free of rotating interference with the stopping block. The dial wheel is fixed on the dial wheel driving piece, the unlocking pinball and the unlocking matching spring are sequentially arranged on the lower portion of the pinball containing groove, the lower end of the unlocking matching spring is matched with the lock cylinder, and the lock cylinder rotates to enable the unlocking pinball to enter or exit from the pinball containing groove. The electronic remote control lock head can be unlocked by an emergency key in emergency circumstances, and the trouble and loss of overall dismounting of the electronic lock are avoided; the concealment of an emergency key hole is good, and the safety is high.

The invention discloses an environment-friendly clay curing agent and its preparation method and use method. The clay curing agent comprises raw materials with the following mass components: 10-50 parts of vegetable oil, 10-30 parts of unsaturated fatty acid potassium salt, 5-50 parts of diffusing agent 20 parts, 0.01-0.5 parts of metal cation sulfate or hydrochloride, 10-50 parts of sodium copolyacrylate, 1-20 parts of pH buffer solution. The curing agent provided by the invention has good permeability, diffusivity, stability and environmental protection. When applied to clay strengthening, the obtained solidified soil can meet the construction requirements of the subgrade base layer and the waterproof layer of the subgrade, which can greatly save engineering construction Reduce costs, improve construction quality, and speed up construction progress.

The invention discloses a sand groove connecting node structure for a free face of a basement. The sand groove connecting node structure is characterized in that a first floor of a central basement is connected to a first floor of a side basement corresponding to each free face through a sand groove structure, so that under a normal working condition with only temperature change, temperature difference deformation release between the central basement and the side basements is achieved, and horizontal force can be transmitted between the central basement and the side basements in a reciprocating mode under the abnormal working conditions of earthquakes and strong wind; moreover, the sand groove structure has no influence on the functions of the basement and the ground building thereof, and the first floor of the basement can be used as a built-in layer of the ground building; and in addition, the sand groove structure is high in mounting precision, simple to mount and operate, easy to construct on site, low in material cost and free of additional mechanical devices, and has the advantages of being simple in later maintenance and outstanding in economic benefit.

The invention belongs to the technical field of a traditional Chinese medicine, particularly relates to a painless quick-acting coaptation medicine which comprises the following raw materials according to the parts by weight: 5 to 25 of mastic, 5 to 35 of native copper, 5 to 10 of aconite root, 5 to 10 of myrrh, 5 to 10 of woodlouse, 5 to 10 of panax notoginseng, 5 to 10 of earthworm, 5 to 10 of safflower, 5 to 10 of borneol, 1 to 5 of angelica root, 1 to 5 of cortex mori radicis, 1 to 5 of radices paeoniae rubra, 0.5 to 1 of vermilion and 1 to 2 of pearl powder. The painless quick-acting coaptation medicine contains no valuable materials, can lead patients to achieve the effects of quick acesodyne, detumescence, coaptation, tendon relaxtion, activeation of blood circulation and stasis elimination and is suitable for various diseases of fracture, blood stasis, sore pain, injuries from falls, and the like. Compared with the like product, the painless quick-acting coaptation medicine has the advantages of quick action, effective treatment, high cure rate, short convalescence, no side effect, and the like, and achieves the total effective rate of 97 percent.