Pseudomonas mendoza and its culture medium, fermentation method and application

A fermentation method and culture medium technology, applied in the field of microorganisms, can solve the problems of low fermentation efficiency and high cost of culture medium, and achieve high-efficiency fermentation and stable and reliable fermentation methods.

Active Publication Date: 2021-04-09
碧沃丰生物科技(广东)股份有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The publication number is CN101787353A, and the invention title is "Pseudomonas mendoza CY004 and its application for efficient removal of nitrite nitrogen, nitrate nitrogen and ammonia nitrogen in water bodies", which discloses a Pseudomonas mendoza of nitrogen, nitrate nitrogen and nitrite nitrogen, the number of viable bacteria after fermentation for 72h was only 1×10 10 CFU / mL, low fermentation efficiency, high cost of medium

Method used

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  • Pseudomonas mendoza and its culture medium, fermentation method and application
  • Pseudomonas mendoza and its culture medium, fermentation method and application

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Effect test

preparation example Construction

[0089] The invention optimizes the preparation method of the fermentation medium, which is specifically realized by limiting the pH value and temperature in the fermentation process.

[0090] The pH of the present invention is set to 7.2-7.8, preferably 7.5, and the pH of the fermentation liquid is controlled by adding ammonia water during the fermentation process, so as to realize high-density fermentation of Pseudomonas mendoza. Table 2 is a comparison of fermentation processes with different pH values. Table 2 is only different in the setting of pH, and the other test conditions are completely consistent.

[0091] Fermentation pH 7.0 7.2 7.5 7.8 8.0 Viable count (10 10 )

5.5 6.1 6.4 6.2 5.8

[0092] As can be seen from Table 2, the pH value of the present invention is set at 7.2-7.8 to have a larger number of viable bacteria. If the pH value is less than 7.0, the number of viable bacteria is 14% lower than that of pH 7.5, and if the pH valu...

Embodiment 1

[0108] 1. Formula:

[0109] Fermentation medium: starch 15g / L, corn steep liquor dry powder 6g / L, dipotassium hydrogen phosphate 4g / L, ammonium sulfate 2g / L, magnesium sulfate heptahydrate 0.1g / L.

[0110] Primary seed medium: peptone 5g / L, glucose 5g / L, yeast powder 3g / L, sodium chloride 3g / L.

[0111] Secondary seed medium: starch 15g / L, corn steep liquor dry powder 6g / L, dipotassium hydrogen phosphate 4g / L, ammonium sulfate 2g / L, magnesium sulfate heptahydrate 0.1g / L.

[0112] Feed medium: starch 150g / L, corn steep liquor dry powder 40g / L, dipotassium hydrogen phosphate 20g / L, ammonium sulfate 10g / L, magnesium sulfate heptahydrate 0.5g / L.

[0113] 2. Fermentation method:

[0114] (1) Prepare shake flask seed solution:

[0115] (1) Pick a single colony of Pseudomonas mendoza freshly cultivated, inoculate it into a primary seed medium, and cultivate it for 10 h at 30° C. and 150 rpm as a primary shake flask seed solution;

[0116] (2) After the first-level shake flask see...

Embodiment 2

[0129] 1. Formula:

[0130] Fermentation medium: starch 20g / L, corn steep liquor dry powder 8g / L, dipotassium hydrogen phosphate 5g / L, ammonium sulfate 3g / L, magnesium sulfate heptahydrate 0.2g / L.

[0131] Primary seed medium: peptone 10g / L, glucose 10g / L, yeast powder 4g / L, sodium chloride 4g / L.

[0132] Secondary seed medium: starch 20g / L, corn steep liquor dry powder 8g / L, dipotassium hydrogen phosphate 5g / L, ammonium sulfate 3g / L, magnesium sulfate heptahydrate 0.2g / L.

[0133] Feed medium: starch 200g / L, corn steep liquor dry powder 50g / L, dipotassium hydrogen phosphate 30g / L, ammonium sulfate 15g / L, magnesium sulfate heptahydrate 1g / L.

[0134] 2. Fermentation method:

[0135] (1) Prepare shake flask seed solution:

[0136] (1) Pick a freshly cultured Pseudomonas mendoza single colony, inoculate it into a primary seed medium, and cultivate it for 12 hours at 35° C. and 200 rpm as a primary shake flask seed solution;

[0137](2) After the first-level shake flask seed ...

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Abstract

The invention discloses a Pseudomonas mendoza, which was preserved in the Guangdong Microbial Culture Collection Center on December 8, 2017, and the biological preservation number is GDMCC 60297. Correspondingly, the present invention also discloses the culture medium of Pseudomonas mendoza, including seed culture medium, fermentation medium and / or feed medium, wherein, fermentation medium comprises: starch 15-30g / L, corn steep liquor Dry powder 6-14g / L, dipotassium hydrogen phosphate 4-8g / L, ammonium sulfate 2-5g / L, magnesium sulfate heptahydrate 0.1-0.4g / L. Correspondingly, the invention also discloses a fermentation method of Pseudomonas mendoza and the application of Pseudomonas mendoza in sewage treatment. By adopting the invention, the low-cost and high-density fermentation of Pseudomonas mendoza is realized.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular to a kind of Pseudomonas mendoza, and the culture medium of the above-mentioned Pseudomonas mendoza, the fermentation process of the above-mentioned Pseudomonas mendoza, the above-mentioned Pseudomonas mendoza Bacteria application. Background technique [0002] The problem of nitrogen pollution is becoming more and more obvious, and its treatment is urgent. For example, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen may be transformed into carcinogenic, mutagenic and teratogenic nitrosamines; another example is the inflow of nitrogen into water bodies to cause eutrophication of water bodies, causing deterioration of water quality and even lake degradation. Biological nitrogen removal has the advantages of good treatment effect, stable and reliable treatment process, and convenient operation and management, so it has been widely used. [0003] Traditional bi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
CPCC02F3/34C12N1/20C12N1/205C12R2001/38
Inventor 夏雨吴焯颖胡亚冬范德朋
Owner 碧沃丰生物科技(广东)股份有限公司
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