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The preparation method of gibberellic acid

A technology of gibberellic acid and magnesium sulfate, which is applied in the field of fermentation, can solve problems that need to be improved, and achieve the effects of ensuring synthesis and metabolism, increasing acid production, and fast and stable fermentation

Active Publication Date: 2021-07-02
SICHUAN LOMON BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing preparation of gibberellic acid usually adopts liquid submerged fermentation method. During the fermentation process, soybean oil or glucose is added in batches or batches. The higher level of fermentation acid production obtained is 2800-3000ppm, but it still needs to be improved.

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  • The preparation method of gibberellic acid
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  • The preparation method of gibberellic acid

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preparation example Construction

[0036] The invention provides a kind of preparation method of gibberellic acid, comprising:

[0037] S1) preparing the seed solution of gibberellic acid;

[0038] S2) Transfer the seed liquid of gibberellic acid into a fermenter for fermentation to obtain a gibberellic acid fermentation liquid; the fermentation medium in the fermenter includes: corn gluten powder 10-40g / L; potassium dihydrogen phosphate 1 ~10g / L; small molecular organic carbon source 5~30g / L; vegetable oil 0.5~5g / L; magnesium sulfate 0.5~2g / L; ammonium sulfate 0.5~2g / L; trace elements 0.1~1g / L; When the dissolved oxygen is higher than 20% to 30%, add glucose and vegetable oil;

[0039] S3) after the gibberellic acid fermentation liquid is concentrated by membrane filtration, a concentrated liquid is obtained;

[0040] S4) After refining the concentrated solution, gibberellic acid is obtained.

[0041] The present invention has no special limitation on the sources of all raw materials, which can be commercia...

Embodiment 1

[0074] 1.1 Will GA 3 The strain freezing tube was directly connected to the shake flask, and the inoculum amount was 0.8% of the volume of the culture medium in the shake flask. The medium formula in the shake flask was shown in Table 1. The fermentation temperature was 29±0.2°C, and the shake flask was cultivated for about 68 hours to obtain a suspension.

[0075] Table 1 Shake flask culture medium formula

[0076]

[0077]

[0078] 1.2 Inoculate the suspension into the primary seed tank, the inoculation amount is 0.016% of the volume of the culture solution, and the medium formula of the primary seed tank is shown in Table 2.

[0079] Table 2 Formula of first-class seed tank culture medium

[0080]

[0081] Fermentation volume: constant volume 1.3t, the volume after digestion is calculated as 1.5t (add sulfuric acid before sterilization to adjust the pH to 5.1).

[0082] Fermentation temperature: 29±0.2°C.

[0083] Operating tank pressure: 0.035MPa.

[0084] Air f...

Embodiment 2

[0137] 2.1 Tubular membrane filtration:

[0138] 120t of fermented liquid obtained in Example 1 was sterilized after fermentation, then transferred to a storage tank, and then directly started tubular membrane filtration (without adjusting the pH). After filtering out the clear liquid of about 45T of the fermentation broth, add tap water continuously, and stop the tubular membrane filtration when the clear liquid of about 260T of the fermented liquid is filtered out. (Specification of tubular membrane: 4KD and material: metal membrane)

[0139] The supernatant liquid from membrane filtration enters the next main process (ultrafiltration), and the residual liquid enters the next auxiliary process (plate and frame filtration).

[0140] 2.2 Plate and frame filter:

[0141] After tubular membrane filtration, the residual liquid of about 35T is directly subjected to plate and frame filtration (the specification of the plate and frame filter membrane is 750B, and the material is p...

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Abstract

The invention provides a preparation method of gibberellic acid, comprising: S1) preparing gibberellic acid seed liquid; S2) transferring the gibberellic acid seed liquid into a fermenter for fermentation to obtain gibberellic acid fermentation liquid; S3 ) concentrating the gibberellic acid fermentation broth through membrane filtration to obtain a concentrated solution; S4) refining the concentrated solution to obtain gibberellic acid. Compared with the prior art, the present invention uses sugar and oil mixed supplementation in the fermentation process to increase the amount of acid production. At the same time, corn gluten powder is used as an organic nitrogen source, and its protein content is higher, reaching more than 50%. High-density fermentation can avoid the hidden dangers of Aspergillus flavus in peanut cake powder, ensure the synthesis and metabolism of bacteria, and enable rapid and stable fermentation; moreover, adding vegetable oil can not only provide carbon sources for fermentation, but also effectively Reduce the occurrence of fermentation bubbles escaping, and avoid potential risks such as material loss and bacterial contamination.

Description

technical field [0001] The invention belongs to the technical field of fermentation, in particular to a preparation method of gibberellic acid. Background technique [0002] Gibberellic acid is a type of secondary metabolite obtained through the fermentation and cultivation of Gibberella. There are 116 species that have been isolated, identified and named, of which GA is the most widely used in agriculture. 3 , plays a huge role in my country's agricultural production. [0003] GA 3 It has very high plant regulation activity, and has obvious regulation effects on the growth and development of various crops. in North, GA 3 It is mainly used in economic crops such as grapes, red dates, hawthorns, nectarines, etc. Spraying a certain concentration of gibberellic acid solution during the flowering period can effectively increase the fruit setting rate of fruit trees, promote fruit growth and development, and increase the yield of fruit trees by about 20% to 30%. In the south,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P27/00C07D307/93
CPCC07D307/93C12P27/00
Inventor 刘健杨宝强熊仁科沈颂娣谢杰景飞江
Owner SICHUAN LOMON BIO TECH CO LTD
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