Pseudomonas mendocina and culture medium, fermentation process and application thereof

A fermentation method and the technology of monocellular bacteria, which are applied in the field of microorganisms, can solve the problems of low fermentation efficiency and high cost of medium, and achieve high-efficiency fermentation and stable and reliable fermentation methods

Active Publication Date: 2018-05-25
碧沃丰生物科技(广东)股份有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Reference document 1 is a patent with the publication number CN101787353A and the invention name "Pseudomonas mendoza CY004 and its application for efficient removal of nitrite nitrogen, nitrate nitrogen and ammonia nitrogen in water bodies", which discloses a patent that can remove ni

Method used

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  • Pseudomonas mendocina and culture medium, fermentation process and application thereof
  • Pseudomonas mendocina and culture medium, fermentation process and application thereof

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Experimental program
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Effect test

preparation example Construction

[0089] The present invention optimizes the preparation method of the fermentation medium, specifically by limiting the pH value and temperature in the fermentation process.

[0090] The pH of the present invention is set at 7.2-7.8, preferably 7.5, and the high-density fermentation of Pseudomonas mendoza can be realized by adding ammonia in the fermentation process to control the pH of the fermentation broth. Table 2 is a comparison of the fermentation processes with different pH values. Table 2 is only different in the setting of pH, and the other test conditions are completely consistent.

[0091] Fermentation pH

7.0

7.2

7.5

7.8

8.0

Viable count (10 10 )

5.5

6.1

6.4

6.2

5.8

[0092] As can be seen from Table 2, the pH value of the present invention is set at 7.2-7.8 to have a larger number of viable bacteria. If the pH value is less than 7.0, the number of viable bacteria is 14% lower than that of pH 7.5, and if ...

Embodiment 1

[0108] 1. Formula:

[0109] Fermentation medium: starch 15g / L, corn steep liquor dry powder 6g / L, dipotassium hydrogen phosphate 4g / L, ammonium sulfate 2g / L, magnesium sulfate heptahydrate 0.1g / L.

[0110] Primary seed medium: peptone 5g / L, glucose 5g / L, yeast powder 3g / L, sodium chloride 3g / L.

[0111] Secondary seed medium: starch 15g / L, corn steep liquor dry powder 6g / L, dipotassium hydrogen phosphate 4g / L, ammonium sulfate 2g / L, magnesium sulfate heptahydrate 0.1g / L.

[0112] Feed medium: starch 150g / L, corn steep liquor dry powder 40g / L, dipotassium hydrogen phosphate 20g / L, ammonium sulfate 10g / L, magnesium sulfate heptahydrate 0.5g / L.

[0113] 2. Fermentation method:

[0114] (1) Prepare shake flask seed solution:

[0115] (1) Pick a single colony of Pseudomonas mendoza that is freshly cultivated, inoculate it into a primary seed medium, and cultivate it for 10 h at 30° C. and 150 rpm as a primary shake flask seed solution;

[0116] (2) After the first-level shake f...

Embodiment 2

[0129] 1. Formula:

[0130] Fermentation medium: starch 20g / L, corn steep liquor dry powder 8g / L, dipotassium hydrogen phosphate 5g / L, ammonium sulfate 3g / L, magnesium sulfate heptahydrate 0.2g / L.

[0131] Primary seed medium: peptone 10g / L, glucose 10g / L, yeast powder 4g / L, sodium chloride 4g / L.

[0132] Secondary seed medium: starch 20g / L, corn steep liquor dry powder 8g / L, dipotassium hydrogen phosphate 5g / L, ammonium sulfate 3g / L, magnesium sulfate heptahydrate 0.2g / L.

[0133] Feed medium: starch 200g / L, corn steep liquor dry powder 50g / L, dipotassium hydrogen phosphate 30g / L, ammonium sulfate 15g / L, magnesium sulfate heptahydrate 1g / L.

[0134] 2. Fermentation method:

[0135] (1) Prepare shake flask seed solution:

[0136] (1) Pick a single colony of Pseudomonas mendoza freshly cultivated, inoculate it into a primary seed medium, and cultivate it for 12 hours at 35°C and 200rpm as a primary shake flask seed solution;

[0137] (2) After the first-level shake flask seed...

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Abstract

The invention discloses a pseudomonas mendocina. The pseudomonas mendocina is preserved in Guangdong Culture Collection Center on December 12, 2017 with a biological preservation number of GDMCC 60297. Correspondingly, the invention also discloses a culture medium of the pseudomonas mendocina. The culture medium of the pseudomonas mendocina comprises a seed medium, a fermentation medium and/or a fed-batch medium, wherein the fermentation medium is composed of 15-30 g/L of starch, 6-14 g/L of dried corn steep liquor powder, 4-8 g/L dipotassium phosphate, 2-5 g/L of ammonium sulfate and 0.1-0.4g/L of magnesium sulfate heptahydrate. Correspondingly, the invention also discloses a fermentation process of the pseudomonas mendocina and application of the pseudomonas mendocina to sewage treatment. The fermentation process of the pseudomonas mendocina can achieve low-cost and high-density fermentation of the pseudomonas mendocina.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular to a kind of Pseudomonas mendoza, and the culture medium of the above-mentioned Pseudomonas mendoza, the fermentation process of the above-mentioned Pseudomonas mendoza, the above-mentioned Pseudomonas mendoza Bacteria applications. Background technique [0002] The problem of nitrogen pollution is becoming more and more obvious, and its treatment is urgent. For example, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen may be transformed into carcinogenic, mutagenic and teratogenic nitrosamines; another example is the influx of nitrogen into water bodies to cause eutrophication of water bodies, causing deterioration of water quality and even lake degradation. Biological denitrification has the advantages of good treatment effect, stable and reliable treatment process, and convenient operation and management, so it has been widely used. [0003] Traditional bi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38
CPCC02F3/34C12N1/20C12N1/205C12R2001/38
Inventor 夏雨吴焯颖胡亚冬范德朋
Owner 碧沃丰生物科技(广东)股份有限公司
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