Enterococcus faecalis high-density fermentation medium and its fermentation process

Abstract

The invention relates to a high-density enterococcus faecalis fermentation culture medium and a fermentation process thereof. The fermentation culture medium is prepared from 20-30 g / L of glycerol, 10-15 g / L of soybean meal, 1-3 g / L of ammonium sulfate, 2-3 g / L of potassium hydrogen phosphate, 3-8 g / L of sodium acetate trihydrate, 2-4 g / L of trisodium citrate dihydrate, 0.3-0.8g / L of magnesium sulfate heptahydrate and 0.1-0.3 g / L of manganese sulfate monohydrate. The fermentation process comprises the steps that high dissolved oxygen (25%) is adopted and combined with glycerin to serve as a fermentation carbon source, the pH value of the constant sodium carbonate fermentation liquid is 5.5, the glycerol concentration is controlled to 10 g / L by adding a supplementary material in the fermentation process in a flowing mode, culture is performed for 16 hours, and then the fermentation is completed when OD (600 nm) is greater than 35, wherein the viable count of the enterococcus faecalis contained in the fermentation liquid can be up to 8*1010 CFU / mL or above. In the fermentation mode that the high dissolved oxygen is combined with the glycerin to serve as the carbon source, the lactic acid concentration in the fermentation liquid is reduced, the enterococcus faecalis contained in the fermentation liquid can be up to 8*1010 CFU / mL or above, the biomass separation cost is reduced, a production cycle is shortened, the production cost is reduced, and the production efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a high-density fermentation medium for Enterococcus faecalis and a fermentation process thereof, which are suitable for high-density fermentation production of Enterococcus faecalis. Background technique [0002] The Catalog of Feed Additives (2013) defines Enterococcus faecalis as a strain that can be added to feed, which can form a biofilm in the intestinal tract of animals, attach to the intestinal mucosa of animals, and develop, grow and reproduce. Enterococcus faecalis belongs to the normal flora in the intestinal tract of humans and animals, and can colonize effectively after entering the intestinal tract. The fermentation of Enterococcus faecalis produces lactic acid, which is beneficial to reduce the pH of the intestinal environment and inhibit the growth of harmful bacteria. During the metabolic process of Enterococcus faecalis, substances such as hydrogen peroxide and...

AI Technical Summary

Problems solved by technology

During the metabolic process of Enterococcus faecalis, substances such as hydrogen peroxide and bacteriocin can also be produced, which have a certain killing effect on pathogenic bacteria, and can produce natural antibiotics, which are beneficial to the health of the organism. Currently, they are widely used in the aquaculture industry, but Traditional Enterococcus faecalis fermentation mostly uses MRS medium, which is expensive and the number of viable bacteria after fermentation is low. In the case of a fixed effective dosage of probiotics, low fermentation level equals a disguised increase in cost

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