High-density fermentation medium for enterococcus faecalis for feed and fermentation method of medium

Abstract

The invention belongs to the technical field of culture of enterococcus faecalis and relates to a high-density fermentation medium for the enterococcus faecalis for feed and a fermentation method of the medium. The strategy that addition of sucrose is controlled according to pH feedback is adopted, and meanwhile, the concentration of lactic acid in fermentation broth is reduced, so that the feedback inhibition effect of lactic acid is relieved; the bacterium density of the enterococcus faecalis fermented by the aid of the high-density fermentation medium with the fermentation method of the medium is remarkably higher than that of enterococcus faecalis fermented with a common fermentation method, the number of living bacteria is not less than 10<10> cfu / mL and is 40 times or more that of enterococcus faecalis cultured by the aid of a common MRS medium, and high-density fermentation of the enterococcus faecalis is realized; thus, the separation cost of biomass can be reduced, the production cycle can be shortened, the production cost can be reduced, and the production efficiency can be improved.

Description

Technical field [0001] The invention belongs to the technical field of Enterococcus faecalis culture, and relates to a high-density fermentation medium of Enterococcus faecalis for feed and a fermentation method thereof. Background technique [0002] Enterococcus faecalis is a Gram-positive, one of the main bacterial groups in the intestines of humans and animals. It can produce natural antibiotics, which is beneficial to the health of the body. It can also produce bacteriostatic substances such as bacteriocins and inhibit pathogenic bacteria such as E. coli and Salmonella. The growth of the intestinal tract can improve the intestinal microenvironment; it can also inhibit the reproduction of urease-producing bacteria and spoilage bacteria in the intestine, reduce the content of intestinal urease and endotoxin, and reduce the content of ammonia and endotoxin in the blood. In addition, Enterococcus is a type of microorganisms that normally exist in the digestive tract. It has stron...

AI Technical Summary

Problems solved by technology

[0004] Enterococcus faecalis is still difficult to achieve high-density fermentation. The main problem that is difficult to achieve is the fermentation medium and its fermentation method. The lack of nutrients and the accumulation of metabolites (mainly lactic acid) in the later stage of fermentation inhibit the growth of bacteria, making it difficult to achieve high-density fermentation.