High-density fermentation medium for enterococcus faecalis for feed and fermentation method of medium

A technology of high-density fermentation and Enterococcus faecalis, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the difficulties of Enterococcus faecalis in achieving high-density fermentation, lack of nutrients, and difficulty in achieving high-density fermentation, etc. Problems, achieve the effect of shortening the production cycle, improving production efficiency and reducing separation costs

Inactive Publication Date: 2016-11-23
JIANGXI KENUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Enterococcus faecalis is still difficult to achieve high-density fermentation. The main problem that is difficult to achieve is the fermentation medium and its fermentation method. The lack of nutrients and the accumulation of metabolites (mainly lactic acid) in the later stage of fermentation inhibit the growth of bacteria, making it difficult to achieve high-density fermentation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of fermentation medium and comparison of fermentation

[0027] 1. The components and contents of Enterococcus faecalis high-density fermentation medium are:

[0028] Sucrose 20g / L, peptone 10g / L, yeast powder 10g / L, K 2 HPO 4 2g / L, sodium citrate 2g / L, MgSO4: 0.2g / L, FeSO4: 0.1g / L, MnSO4: 55mg / L.

[0029] 2. Medium preparation method:

[0030] (1) Weigh each component according to the above content and dissolve it with distilled water, in which sucrose is dissolved separately;

[0031] (2) Sucrose was sterilized separately at 115°C for 20 minutes, and the other components were put into the fermentation tank at 121°C and sterilized for 30 minutes;

[0032] (3) Before inoculation, add sucrose to the fermentor through aseptic operation, stir and mix well, and get it.

[0033] 3 Comparison of high-density fermentation and existing technology fermentation

[0034] (1) Activate the frozen Enterococcus faecalis on the MRS agar plate three times, pick a single colony ...

Embodiment 2

[0049] Example 2 Preparation of fermentation medium and comparison of fermentation

[0050] 1. The components and contents of Enterococcus faecalis high-density fermentation medium are:

[0051] Sucrose 20g / L, peptone 15g / L, yeast powder 15g / L, K 2 HPO 4 3g / L, sodium citrate 4g / L, MgSO4: 0.2g / L, FeSO4: 0.1g / L, MnSO4: 80mg / L.

[0052] 2. Medium preparation method:

[0053] (1) Weigh each component according to the above content and dissolve it with distilled water, in which sucrose is dissolved separately;

[0054] (2) Sucrose was sterilized separately at 115°C for 20 minutes, and the other components were put into the fermentation tank at 121°C and sterilized for 30 minutes;

[0055] (3) Before inoculation, add sucrose to the fermentor through aseptic operation, stir and mix well, and get it.

[0056] 3 Comparison of high-density fermentation and existing technology fermentation

[0057] (1) Activate the frozen Enterococcus faecalis on the MRS agar plate three times, pick a single colony ...

Embodiment 3

[0072] Example 3 Preparation of fermentation medium and comparison of fermentation

[0073] 1. The components and contents of Enterococcus faecalis high-density fermentation medium are:

[0074] Sucrose 30g / L, peptone 15g / L, yeast powder 25g / L, K 2 HPO 4 2g / L, sodium citrate 4g / L, MgSO4: 0.2g / L, FeSO4: 0.1g / L, MnSO4: 60mg / L.

[0075] 2. Medium preparation method:

[0076] (1) Weigh each component according to the above content and dissolve it with distilled water, in which sucrose is dissolved separately;

[0077] (2) Sucrose was sterilized separately at 115°C for 20 minutes, and the other components were put into the fermentation tank at 121°C and sterilized for 30 minutes;

[0078] (3) Before inoculation, add sucrose to the fermentor through aseptic operation, stir and mix well, and get it.

[0079] 3 Comparison of high-density fermentation and existing technology fermentation

[0080] (1) Activate the frozen Enterococcus faecalis on the MRS agar plate three times, pick a single colony a...

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PUM

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Abstract

The invention belongs to the technical field of culture of enterococcus faecalis and relates to a high-density fermentation medium for the enterococcus faecalis for feed and a fermentation method of the medium. The strategy that addition of sucrose is controlled according to pH feedback is adopted, and meanwhile, the concentration of lactic acid in fermentation broth is reduced, so that the feedback inhibition effect of lactic acid is relieved; the bacterium density of the enterococcus faecalis fermented by the aid of the high-density fermentation medium with the fermentation method of the medium is remarkably higher than that of enterococcus faecalis fermented with a common fermentation method, the number of living bacteria is not less than 10<10> cfu/mL and is 40 times or more that of enterococcus faecalis cultured by the aid of a common MRS medium, and high-density fermentation of the enterococcus faecalis is realized; thus, the separation cost of biomass can be reduced, the production cycle can be shortened, the production cost can be reduced, and the production efficiency can be improved.

Description

Technical field [0001] The invention belongs to the technical field of Enterococcus faecalis culture, and relates to a high-density fermentation medium of Enterococcus faecalis for feed and a fermentation method thereof. Background technique [0002] Enterococcus faecalis is a Gram-positive, one of the main bacterial groups in the intestines of humans and animals. It can produce natural antibiotics, which is beneficial to the health of the body. It can also produce bacteriostatic substances such as bacteriocins and inhibit pathogenic bacteria such as E. coli and Salmonella. The growth of the intestinal tract can improve the intestinal microenvironment; it can also inhibit the reproduction of urease-producing bacteria and spoilage bacteria in the intestine, reduce the content of intestinal urease and endotoxin, and reduce the content of ammonia and endotoxin in the blood. In addition, Enterococcus is a type of microorganisms that normally exist in the digestive tract. It has stron...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/46
Inventor 王劲松张魁
Owner JIANGXI KENUO BIOTECH
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