Preparation method of gibberellic acid
A technology of gibberellic acid and magnesium sulfate, which is applied in the field of fermentation and can solve problems that need to be improved
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[0036] The invention provides a kind of preparation method of gibberellic acid, comprising:
[0037] S1) preparing the seed solution of gibberellic acid;
[0038] S2) Transfer the seed liquid of gibberellic acid into a fermenter for fermentation to obtain a gibberellic acid fermentation liquid; the fermentation medium in the fermenter includes: corn gluten powder 10-40g / L; potassium dihydrogen phosphate 1 ~10g / L; small molecular organic carbon source 5~30g / L; vegetable oil 0.5~5g / L; magnesium sulfate 0.5~2g / L; ammonium sulfate 0.5~2g / L; trace elements 0.1~1g / L; When the dissolved oxygen is higher than 20% to 30%, add glucose and vegetable oil;
[0039] S3) after the gibberellic acid fermentation liquid is concentrated by membrane filtration, a concentrated liquid is obtained;
[0040] S4) After refining the concentrated solution, gibberellic acid is obtained.
[0041] The present invention has no special limitation on the sources of all raw materials, which can be commercia...
Embodiment 1
[0074] 1.1 Will GA 3 The strain freezing tube was directly connected to the shake flask, and the inoculum amount was 0.8% of the volume of the culture medium in the shake flask. The medium formula in the shake flask was shown in Table 1. The fermentation temperature was 29±0.2°C, and the shake flask was cultivated for about 68 hours to obtain a suspension.
[0075] Table 1 Shake flask culture medium formula
[0076]
[0077]
[0078] 1.2 Inoculate the suspension into the primary seed tank, the inoculation amount is 0.016% of the volume of the culture solution, and the medium formula of the primary seed tank is shown in Table 2.
[0079] Table 2 Formula of first-class seed tank culture medium
[0080]
[0081] Fermentation volume: constant volume 1.3t, the volume after digestion is calculated as 1.5t (add sulfuric acid before sterilization to adjust the pH to 5.1).
[0082] Fermentation temperature: 29±0.2°C.
[0083] Operating tank pressure: 0.035MPa.
[0084] Air f...
Embodiment 2
[0137] 2.1 Tubular membrane filtration:
[0138] 120t of fermented liquid obtained in Example 1 was sterilized after fermentation, then transferred to a storage tank, and then directly started tubular membrane filtration (without adjusting the pH). After filtering out the clear liquid of about 45T of the fermentation broth, add tap water continuously, and stop the tubular membrane filtration when the clear liquid of about 260T of the fermented liquid is filtered out. (Specification of tubular membrane: 4KD and material: metal membrane)
[0139] The supernatant liquid from membrane filtration enters the next main process (ultrafiltration), and the residual liquid enters the next auxiliary process (plate and frame filtration).
[0140] 2.2 Plate and frame filter:
[0141] After tubular membrane filtration, the residual liquid of about 35T is directly subjected to plate and frame filtration (the specification of the plate and frame filter membrane is 750B, and the material is p...
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