Bacillus mucilaginosus and culture method and culture medium thereof
The technology of a bacillus and a culture method, applied in the field of microorganisms, can solve the problems of complex production technology and process control, and achieve the effects of shortening fermentation time, strengthening functional activity, and improving crop yield
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Embodiment 1
[0036] Example 1: Obtaining of Paenibacillus jelly-like P.muc 3016:
[0037] 1. Separation location and environment:
[0038] As a specific embodiment of the present invention, the separation site and environment select the soil in the inner plow layer of the national brown fluvo-aquic soil fertility and fertilizer benefit long-term monitoring base in Changping District, Beijing. The base is located at 40 ° 13' north latitude and 116 ° 14' east longitude; The physical and chemical properties are listed in Table 1.
[0039] Table 1 Soil condition of isolated area
[0040]
[0041] 2. Strain screening
[0042] (1), pick typical colonies:
[0043] Weigh 10g of soil and add it into 100ml of sterile water, shake on a shaker at 25°C for 30 minutes, take the supernatant as 10 -1 ~10 -4Dilution, each dilution was treated at 80°C for 15 minutes, 0.1ml was taken and spread on the screening plate medium, cultured at 28°C-30°C for 3-5 days, and a single colony with the typical c...
Embodiment 2
[0057] Example 2: Nitrogen fixation, phosphorus dissolving and potassium dissolving properties of strain P.muc 3016:
[0058] According to the assay method of nitrogen fixation, phosphorus dissolution and potassium solution in the bacterial strain screening method of Example 1, the nitrogen fixation, phosphorus dissolution and potassium solution performance of bacterial classification P.muc 3016 are measured. Nitrogenase activity of 3.024C 2 h 4 nmol / (h -1 mg) protein, which is significantly higher than the nitrogenase activity of the rotund nitrogen-fixing bacteria ACCC 11103 used in the production of microbial fertilizers and reaches 85%; In the phosphorus-deficient medium of lecithin (organophosphorus), the content of inorganic and organic phosphorus increased by 211.5% and 146.2% respectively compared with the control; The concentration of water-soluble potassium was 87.7mg / L, compared with 23.4mg / L in the control, an increase of 3.75 times.
Embodiment 3
[0059] Example 3: Culture of bacteria:
PUM
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