Bacillus mucilaginosus and culture method and culture medium thereof

The technology of a bacillus and a culture method, applied in the field of microorganisms, can solve the problems of complex production technology and process control, and achieve the effects of shortening fermentation time, strengthening functional activity, and improving crop yield

Inactive Publication Date: 2011-02-09
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing microbial fertilizers (bacteria agents) with the ability to fix nitrogen, dissolve phosphorus, and dissolve potassium all use mixed strains to achieve these three functions, that is, the strains with nitrogen fixation ability, the strain with phosphorus Potassium-capable strains are composed of three types of strains, and the corresponding production process and process control are more complicated

Method used

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  • Bacillus mucilaginosus and culture method and culture medium thereof
  • Bacillus mucilaginosus and culture method and culture medium thereof
  • Bacillus mucilaginosus and culture method and culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Obtaining of Paenibacillus jelly-like P.muc 3016:

[0037] 1. Separation location and environment:

[0038] As a specific embodiment of the present invention, the separation site and environment select the soil in the inner plow layer of the national brown fluvo-aquic soil fertility and fertilizer benefit long-term monitoring base in Changping District, Beijing. The base is located at 40 ° 13' north latitude and 116 ° 14' east longitude; The physical and chemical properties are listed in Table 1.

[0039] Table 1 Soil condition of isolated area

[0040]

[0041] 2. Strain screening

[0042] (1), pick typical colonies:

[0043] Weigh 10g of soil and add it into 100ml of sterile water, shake on a shaker at 25°C for 30 minutes, take the supernatant as 10 -1 ~10 -4Dilution, each dilution was treated at 80°C for 15 minutes, 0.1ml was taken and spread on the screening plate medium, cultured at 28°C-30°C for 3-5 days, and a single colony with the typical c...

Embodiment 2

[0057] Example 2: Nitrogen fixation, phosphorus dissolving and potassium dissolving properties of strain P.muc 3016:

[0058] According to the assay method of nitrogen fixation, phosphorus dissolution and potassium solution in the bacterial strain screening method of Example 1, the nitrogen fixation, phosphorus dissolution and potassium solution performance of bacterial classification P.muc 3016 are measured. Nitrogenase activity of 3.024C 2 h 4 nmol / (h -1 mg) protein, which is significantly higher than the nitrogenase activity of the rotund nitrogen-fixing bacteria ACCC 11103 used in the production of microbial fertilizers and reaches 85%; In the phosphorus-deficient medium of lecithin (organophosphorus), the content of inorganic and organic phosphorus increased by 211.5% and 146.2% respectively compared with the control; The concentration of water-soluble potassium was 87.7mg / L, compared with 23.4mg / L in the control, an increase of 3.75 times.

Embodiment 3

[0059] Example 3: Culture of bacteria:

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Abstract

The invention relates to a kind of Bacillus mucilaginosus and a culture method and culture medium thereof, belonging to the microbiological technical field. The Bacillus mucilaginosus P.muc3016 has the functions of nitrogen fixation, phosphorus-dissolving and potassium-releasing and belongs to the Paenibacillus group. The batch fermentation method is adopted to ferment and culture the Bacillus mucilaginosus and the concentrations of carbon source and dissolved oxygen are monitored and regulated. The culture medium contains carbon source, soybean meal powder, ammonium sulfate, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium sulfate and calcium carbonate, and the pH value of the culture medium is 7.5-7.0. The Bacillus mucilaginosus of the invention has three functions of nitrogen fixation, phosphorus-dissolving and potassium-releasing simultaneously, has higher function activity and can obviously increase the crop yield; and by adopting the culture method and culture medium of the invention, the fermentation density can be increased and the fermentation time can be shortened.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a jelly-like Paenibacillus P.muc3016CGMCC No.4100, a method and a culture medium for cultivating the bacterium. Background technique [0002] At present, the shortage of phosphorus and potassium in agricultural soil in our country is becoming more and more obvious. On the one hand, the production capacity of phosphorus and potassium fertilizers in my country is seriously insufficient, and a large amount of imports are required every year; on the other hand, the soil in the plow layer contains a large amount of phosphorus and potassium, but most of these phosphorus and potassium are solidified in aluminosilicate , cannot be absorbed by plants. In addition, a large amount of nitrogen fertilizers are currently used in agricultural production in our country, but the utilization rate of nitrogen fertilizers is less than 40%. A large amount of unused nitrogen not onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 李俊李力沈德龙马鸣超姜昕关大伟曹风明陈慧君冯瑞华葛一凡邴晓会杨小红
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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